2011
DOI: 10.1063/1.3605508
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An electroactive microwell array for trapping and lysing single-bacterial cells

Abstract: Interest in single-cell analysis has increased because it allows to understand cell metabolism and characterize disease states, cellular adaptation to environmental changes, cell cycles, etc. Here, the authors propose a device to electrically trap and lyse single-bacterial cells in an array format for high-throughput single-cell analysis. The applied electric field is highly deformed and concentrated toward the inside of the microwell structures patterned on the planar electrode. This configuration effectively… Show more

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Cited by 23 publications
(20 citation statements)
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“…To achieve the controllability of complex genetic circuits, genetic parts such as riboswitches should be developed. Technologies such as the electroactive microwell array [31] are also required to assess more states of cells, for measurability. Furthermore, for systematic control, a linear approximation of a mathematical model of cells with a synthetic circuit, such as piecewise affine approximation [32], would be required, because a non-linear system is difficult to control systematically.…”
Section: Discussionmentioning
confidence: 99%
“…To achieve the controllability of complex genetic circuits, genetic parts such as riboswitches should be developed. Technologies such as the electroactive microwell array [31] are also required to assess more states of cells, for measurability. Furthermore, for systematic control, a linear approximation of a mathematical model of cells with a synthetic circuit, such as piecewise affine approximation [32], would be required, because a non-linear system is difficult to control systematically.…”
Section: Discussionmentioning
confidence: 99%
“…Recent advances in microfluidics technologies have opened the door to cancer biophysics and the capture and downstream analyses of circulating tumor cells (Becker, 2009; Beebe et al, 2002; Bhagat et al, 2010; Gossett et al, 2012; Haeberle and Zengerle, 2007; Hansen and Quake, 2003; Kim et al, 2011; Qin, 2012; Qin et al, 2008). A number of groups have published microfluidic approaches for screening the biophysical properties of suspended cells.…”
Section: Microfluidics As a Tool To Study Cell Biophysicsmentioning
confidence: 99%
“…More recently, other methods have emerged, that can separate cells according to differences in their physical parameters: hydrodynamic sorting [12], lateral displacement filtration [13], magnetophoresis [14], electrophoresis [15], acoustophoresis [16] or optical forces [17], AC electrokinetic techniques [18]. In particular, the use of dielectrophoresis forces, electrorotation, and travelling wave dielectrophoresis showed to be quite efficient for cell trapping [19][20][21], cell separation [22][23][24]and cell characterization [25,26]. These label-free and non-contact techniques offer the advantage of being minimally invasive and less sensitive to the inherent pitfalls of surface-mediated capture.…”
Section: Introductionmentioning
confidence: 99%