An electrochemical aptasensor for detection of lead ions using a screen-printed carbon electrode modified with Au/polypyrrole composites and toluidine blue

Ding, Jingtao; Zhang, Dongwei; Liu, Yang; Yu, Minglei; Zhan, Xuejia; Zhang, Dan; Zhou, Pei

doi:10.1039/c9ay01256g

Cited by 39 publications

(22 citation statements)

References 43 publications

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“…In an effort to address these challenges, Ding et al, described two aptamer-based sensors for the detection of lead (II) in soil samples (Ding et al, 2018(Ding et al, , 2019. The most recent sensor takes advantage of the interaction between a G-rich lead binding aptamer, AuNPs, and polypyrrole screen printed onto an electrode to generate an electrochemical signal.…”

Section: Monitoring Lead In Soil Using Aptamer-based Biosensorsmentioning

confidence: 99%

Aptamer-Based Biosensors for Environmental Monitoring

2020

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Due to their relative synthetic and chemical simplicity compared to antibodies, aptamers afford enhanced stability and functionality for the detection of environmental contaminants and for use in environmental monitoring. Furthermore, nucleic acid aptamers can be selected for toxic targets which may prove difficult for antibody development. Of particular relevance, aptamers have been selected and used to develop biosensors for environmental contaminants such as heavy metals, small-molecule agricultural toxins, and water-borne bacterial pathogens. This review will focus on recent aptamer-based developments for the detection of diverse environmental contaminants. Within this domain, aptamers have been combined with other technologies to develop biosensors with various signal outputs. The goal of much of this work is to develop cost-effective, user-friendly detection methods that can complement or replace traditional environmental monitoring strategies. This review will highlight recent examples in this area. Additionally, with innovative developments such as wearable devices, sentinel materials, and lab-on-a-chip designs, there exists significant potential for the development of multifunctional aptamer-based biosensors for environmental monitoring. Examples of these technologies will also be highlighted. Finally, a critical perspective on the field, and thoughts on future research directions will be offered.

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Section: Monitoring Lead In Soil Using Aptamer-based Biosensorsmentioning

confidence: 99%

Aptamer-Based Biosensors for Environmental Monitoring

2020

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“…Recently, enzymes such as HRP, glucose oxidase, and alkaline phosphatase, and electroactive compounds such as QDs, ferrocene (Fc), ferrocyanide, methylene blue (MB), and Cd nanoparticles were successfully incorporated in electrochemical aptasensor technologies to be used as signal enhancers [ 114 , 194 ]. A different strategy was integrated in an electrochemical aptasensor for assaying different targets—AFB1-activated protein C, OTC, patulin (PAT), thrombin, and Pb 2+ , among others—by monitoring the electrochemical signals of the released labeling substrates as a result of competitive targets [ 13 , 43 , 195 , 196 , 197 , 198 , 199 , 200 ]. A portable electrochemical aptasensor was fabricated for assaying OTC using a AuNP/cMWCNT/cDNA@thionine probe as a signal-releasing tag in the presence of the target [ 196 ], as outlined in Figure 10 A.…”

Section: Electrochemical Aptasensormentioning

confidence: 99%

“…A homogeneous electrochemical aptasensor was fabricated based on the departure of a target-responsive label, the electroactive dye methylene blue (MB), from an aptamer-gated zeolitic imidazolate framework-8 (ZIF-8) as a function of the thrombin concentration ( Figure 10 C), with a linear range of 1 fM to 1 nM and LOD of 0.57 fM [ 13 ]. Employing the same technology, Pb 2+ was detected with a range of 0.5–25 ppb and a LOD of 0.6 ppb, using toluidine blue as a redox-released tag signal liberated in the presence of Pb 2+ [ 197 ]. Enzymatic catalysis was incorporated in an electrochemical aptasensor for signal amplification [ 201 , 202 ].…”

Section: Electrochemical Aptasensormentioning

confidence: 99%

Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

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Recently, aptamers have attracted attention in the biosensing field as signal recognition elements because of their high binding affinity toward specific targets such as proteins, cells, small molecules, and even metal ions, antibodies for which are difficult to obtain. Aptamers are single oligonucleotides generated by in vitro selection mechanisms via the systematic evolution of ligand exponential enrichment (SELEX) process. In addition to their high binding affinity, aptamers can be easily functionalized and engineered, providing several signaling modes such as colorimetric, fluorometric, and electrochemical, in what are known as aptasensors. In this review, recent advances in aptasensors as powerful biosensor probes that could be used in different fields, including environmental monitoring, clinical diagnosis, and drug monitoring, are described. Advances in aptamer-based colorimetric, fluorometric, and electrochemical aptasensing with their advantages and disadvantages are summarized and critically discussed. Additionally, future prospects are pointed out to facilitate the development of aptasensor technology for different targets.

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“…The best example of such are gold nanoparticles (AuNPs) and their composites. They are still very popular in the electrochemical aptasensors development because of their unique physical and chemical properties, such as a large surface area, a strong plasmonic characteristic, and easy functionalization with the use of self-organized monolayers [ 13 , 49 , 62 , 73 , 95 , 148 , 158 , 184 , 185 , 186 ]. This allowed for aptasensors development with excellent analytical performances and the cost-effective detection of various types of analytes in different types of matrixes with the use of conventional electrochemical measurement techniques such as cyclic voltammetry (CV), DPV, square wave voltammetry (SWV).…”

Section: Possible Development Directions Of Electrochemical Aptasementioning

confidence: 99%

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

Ziółkowski

Jarczewska

Górski

et al. 2021

Sensors

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This paper focuses on the current state of art as well as on future trends in electrochemical aptasensors application in medical diagnostics. The origin of aptamers is presented along with the description of the process known as SELEX. This is followed by the description of the broad spectrum of aptamer-based sensors for the electrochemical detection of various diagnostically relevant analytes, including metal cations, abused drugs, neurotransmitters, cancer, cardiac and coagulation biomarkers, circulating tumor cells, and viruses. We described also possible future perspectives of aptasensors development. This concerns (i) the approaches to lowering the detection limit and improvement of the electrochemical aptasensors selectivity by application of the hybrid aptamer–antibody receptor layers and/or nanomaterials; and (ii) electrochemical aptasensors integration with more advanced microfluidic devices as user-friendly medical instruments for medical diagnostic of the future.

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An electrochemical aptasensor for detection of lead ions using a screen-printed carbon electrode modified with Au/polypyrrole composites and toluidine blue

Abstract: An aptasensor based on the aptamer of lead(ii) ions and the composites of Au nanoparticles and polypyrrole (Au@Py) was successfully fabricated.

Cited by 39 publications

References 43 publications

Aptamer-Based Biosensors for Environmental Monitoring

Aptamer-Based Biosensors for Environmental Monitoring

Recent Advances in Aptamer Sensors

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

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