An electrochemical biosensor for DNA detection based on tungsten disulfide/multi-walled carbon nanotube composites and hybridization chain reaction amplification

Li, Xue; Shuai, Honglei; Liu, Yujie; Huang, Ke-Jing

doi:10.1016/j.snb.2016.05.132

Cited by 86 publications

(28 citation statements)

References 32 publications

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“…It is also possible to immobilise nucleic acids at CNT-functionalised electrodes using gold/thiol interactions. Liu et al [ 167 ] drop-cast a composite of MWCNTs and tungsten disulphide onto glassy carbon electrodes and formed gold nanoparticles on the surface of the composite via electroformation. Thiolated oligonucleotide capture probes are then bonded to the gold nanoparticles and the hybridisation of the analyte sequence detected via a HRP labelled probe.…”

Section: Biosensors Based On Carbon Nanotubesmentioning

confidence: 99%

Carbon Nanotube (CNT)-Based Biosensors

2021

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This review focuses on recent advances in the application of carbon nanotubes (CNTs) for the development of sensors and biosensors. The paper discusses various configurations of these devices, including their integration in analytical devices. Carbon nanotube-based sensors have been developed for a broad range of applications including electrochemical sensors for food safety, optical sensors for heavy metal detection, and field-effect devices for virus detection. However, as yet there are only a few examples of carbon nanotube-based sensors that have reached the marketplace. Challenges still hamper the real-world application of carbon nanotube-based sensors, primarily, the integration of carbon nanotube sensing elements into analytical devices and fabrication on an industrial scale.

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Section: Biosensors Based On Carbon Nanotubesmentioning

confidence: 99%

Carbon Nanotube (CNT)-Based Biosensors

2021

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“…As a result of the hybridization chain reaction, the multiple biotin anchoring points could bind specifically with multiple avidin-HRP conjugates to increase the signal response of the biosensor. After the optimization steps, the performance of the biosensor was tested by DPV, with different target concentrations, showing a linear response from 10 fM to 0.1 nM, with a LOD of 2.5 fM [ 112 ].…”

Section: Nanobioconjugates In Biosensingmentioning

confidence: 99%

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

Cajigas

Orozco

2020

Molecules

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Nanobioconjugates are hybrid materials that result from the coalescence of biomolecules and nanomaterials. They have emerged as a strategy to amplify the signal response in the biosensor field with the potential to enhance the sensitivity and detection limits of analytical assays. This critical review collects a myriad of strategies for the development of nanobioconjugates based on the conjugation of proteins, antibodies, carbohydrates, and DNA/RNA with noble metals, quantum dots, carbon- and magnetic-based nanomaterials, polymers, and complexes. It first discusses nanobioconjugates assembly and characterization to focus on the strategies to amplify a biorecognition event in biosensing, including molecular-, enzymatic-, and electroactive complex-based approaches. It provides some examples, current challenges, and future perspectives of nanobioconjugates for the amplification of signals in electrochemical biosensing.

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“…For the detection of atrazine, a widely used pesticide in agriculture, Liu et al (2014) developed a simple, specific, sensitive electrochemical immunosensor that is characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) using ferricyanide as an electrochemical redox indicator. This biosensor uses immobilized gold nanoparticles (GNPs) on a gold electrode surface and under favorable condition the limit of detection for atrazine is just 0.016 ng/mL (Liu et al, 2016).…”

Section: Biosensor-advanced Diagnostic Approaches For Rapid Specificmentioning

confidence: 99%

Advanced Diagnostic Approaches for Necrotrophic Fungal Pathogens of Temperate Legumes With a Focus on Botrytis spp.

2019

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Plant pathogens reduce global crop productivity by up to 40% per annum, causing enormous economic loss and potential environmental effects from chemical management practices. Thus, early diagnosis and quantitation of the causal pathogen species for accurate and timely disease control is crucial. Botrytis Gray Mold (BGM), caused by Botrytis cinerea and B. fabae , can seriously impact production of temperate grain legumes separately or within a complex. Accordingly, several immunogenic and molecular probe-type protocols have been developed for their diagnosis, but these have varying levels of species-specificity, sensitivity and consequent usefulness within the paddock. To substantially improve speed, accuracy and sensitivity, advanced nanoparticle-based biosensor approaches have been developed. These novel methods have made enormous impact toward disease diagnosis in the medical sciences and offer potential for transformational change within the field of plant pathology and disease management, with early and accurate diagnosis at the point-of-care in the field. Here we review several recently developed diagnostic tools that build on traditional approaches and are available for pathogen diagnosis, specifically for Botrytis spp. diagnostic applications. We then identify the specific gaps in knowledge and current limitations to these existing tools.

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An electrochemical biosensor for DNA detection based on tungsten disulfide/multi-walled carbon nanotube composites and hybridization chain reaction amplification

Cited by 86 publications

References 32 publications

Carbon Nanotube (CNT)-Based Biosensors

Carbon Nanotube (CNT)-Based Biosensors

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

Advanced Diagnostic Approaches for Necrotrophic Fungal Pathogens of Temperate Legumes With a Focus on Botrytis spp.

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