An Electrochemical DNA Biosensor for Carcinogenicity of Anticancer Compounds Based on Competition between Methylene Blue and Oligonucleotides

Sani, Nor Diyana Md; Ariffin, Eda Yuhana; Wong, Sheryn; Shamsuddin, Mohd Asyraf; Heng, Lee Yook; Latip, Jalifah; Hasbullah, Siti Aishah; Hassan, Nurul Izzaty

doi:10.3390/s19235111

Cited by 16 publications

(7 citation statements)

References 45 publications

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“…On the other hand, at the ionic strength of the solution 0.1 M the shift toward high temperature region for the complexes poly(rA)poly(rU)-EtBr is much higher, than that for MB. These data are in good accordance with the obtained ones in the work [18], concerning to the interaction of the mentioned ligands with ds-DNA. Our obtained data also allow to conclude that for EtBr intercalation, ds-structure of hybrid poly(rA)-poly(rU) is more available at the ionic strength of the solution 0.1 M, than that for MB.…”

Section: Methodssupporting

confidence: 92%

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STUDY OF HYBRIDIZATION OF COMPLEMENTARY SINGLE-STRANDED POLYNUCLEOTIDES POLY(rA) AND POLY(rU)

Movsisyan

2023

Proc. YSU B: Chem. Biol. Sci.

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The study of the interaction of intercalators ethidium bromide (EtBr), methylene blue (MB) and groove binding compound Hoechst 33258 (H33258) with single-stranded synthetic polyribonucleotides poly(rA) and poly(rU) has been carried out by the method of UV-melting, at ionic strengths of the solution 0.04 M and 0.1 M. Stirring of poly(rA) and poly(rU) by equal-molar concentrations was shown to result in hybridization with formation of double-stranded (ds-) structure poly(rA)-poly(rU). It was revealed that EtBr, with higher degree in comparison with MB, stimulates hybridization and stabilizes the formed ds-structure poly(rA)-poly(rU). It was also found out that the hybridization process and affinity of EtBr and MB depend on the ionic strength of the solution and these processes occur much more effectively at the ionic strength 0.04 M. On the other hand, it was shown that the groove binding ligand H33258 practically does not affect the stabilization of the formed ds-structure poly(rA)-poly(rU).

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Section: Methodssupporting

confidence: 92%

“…Though, hybridization occurs practically completely with formation of ds-structure, as a result of which the hypochromic degree composes 36-40%. It should be mentioned that the hyperchromic degree of ds-poly(rA)poly(rU) at denaturation is about 35-36%, as it was obtained in [18].…”

Section: Methodsmentioning

confidence: 72%

STUDY OF HYBRIDIZATION OF COMPLEMENTARY SINGLE-STRANDED POLYNUCLEOTIDES POLY(rA) AND POLY(rU)

Movsisyan

2023

Proc. YSU B: Chem. Biol. Sci.

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“…Many electrochemical biosensors containing DNA structures, such as dsDNA, ssDNA (single-stranded DNA), aptamers, oligonucleotides, and hairpins, detect various analytes and pathogens [ 32 , 33 ], but few studies have been devoted to monitoring the toxicity of these analytes in the DNA structure. An electrochemical DNA biosensor was used for detection of carcinogenicity [ 34 ]. In another study, Machini et al used dsDNA electrochemical biosensors to monitor the drug metformin–dsDNA interaction based on changes in the oxidation peak current of DNA base residues.…”

Section: Introductionmentioning

confidence: 99%

Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor

Blaškovičová

Vyskočil

Augustín

et al. 2023

Sensors

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Engineered nanomaterials are becoming increasingly common in commercial and consumer products and pose a serious toxicological threat. Exposure of human organisms to nanomaterials can occur by inhalation, oral intake, or dermal transport. Together with the consumption of alcohol in the physiological environment of the body containing NaCl, this has raised concerns about the potentially harmful effects of ingested nanomaterials on human health. Although gold nanoparticles (AuNPs) exhibit great potential for various biomedical applications, there is some inconsistency in the case of the unambiguous genotoxicity of AuNPs due to differences in their shape, size, solubility, and exposure time. A DNA/GCE (DNA/glassy carbon electrode) biosensor was used to study ethanol (EtOH) and NaCl-induced gold nanoparticle aggregation genotoxicity under UV light in this study. The genotoxic effect of dispersed and aggregated negatively charged gold nanoparticles AuNP1 (8 nm) and AuNP2 (30 nm) toward salmon sperm double-stranded dsDNA was monitored by cyclic and square-wave voltammetry (CV, SWV). Electrochemical impedance spectroscopy (EIS) was used for a surface study of the biosensor. The aggregation of AuNPs was monitored by UV-vis spectroscopy. AuNP1 aggregates formed by 30% v/v EtOH and 0.15 mol·L−1 NaCl caused the greatest damage to the biosensor DNA layer.

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“…In addition, methylene blue (MB) as an electrochemical indicator 43 was employed to design electrochemical DNA biosensor. 44 CDs/GO nanocomposites accelerate electron transfers between MB and the electrode, and expand the surface of the working electrode to load greater mass of the capture probe DNA. Different pulse voltammetry (DPV) was employed to study the interaction of MB with DNA.…”

Section: Introductionmentioning

confidence: 99%

Efficient Determination of PML/RARα Fusion Gene by the Electrochemical DNA Biosensor Based on Carbon Dots/Graphene Oxide Nanocomposites

Zhang¹,

Huang²,

Xu³

et al. 2021

IJN

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Purpose: The PML/RARα fusion gene as a leukemogenesis plays a significant role in clinical diagnosis of the early stage of acute promyelocytic leukemia (APL). Here, we present an electrochemical biosensor for PML/RARα fusion gene detection using carbon dots functionalized graphene oxide (CDs/GO) nanocomposites modified glassy carbon electrode (CDs/GO/GCE). Materials and Methods: In this work, the CDs/GO nanocomposites are produced through π-π stacking interaction and could be prepared in large quantities by a facile and economical way. The CDs/GO nanocomposites were decorated onto electrode surface to improve the electrochemical activity and as a bio-platform attracted the target deoxyribonucleic acid (DNA) probe simultaneously. Results: The CDs/GO/GCE was fabricated successfully and exhibits high electrochemical activity, good biocompatibility, and strong bioaffinity toward the target DNA sequences, compared with only the pristine CDs on GCE or GO on GCE. The DNA biosensor displays excellent sensing performance for detecting the relevant pathogenic DNA of APL with a detection limit of 83 pM (S/N = 3). Conclusion:According to the several experimental results, we believe that the simple and economical DNA biosensor has the potential to be an effective and powerful tool for detection of pathogenic genes in the clinical diagnosis.

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An Electrochemical DNA Biosensor for Carcinogenicity of Anticancer Compounds Based on Competition between Methylene Blue and Oligonucleotides

Cited by 16 publications

References 45 publications

STUDY OF HYBRIDIZATION OF COMPLEMENTARY SINGLE-STRANDED POLYNUCLEOTIDES POLY(rA) AND POLY(rU)

STUDY OF HYBRIDIZATION OF COMPLEMENTARY SINGLE-STRANDED POLYNUCLEOTIDES POLY(rA) AND POLY(rU)

Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor

Efficient Determination of PML/RARα Fusion Gene by the Electrochemical DNA Biosensor Based on Carbon Dots/Graphene Oxide Nanocomposites

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