An electrochemical immunosensor for ochratoxin A based on immobilization of antibodies on diazonium-functionalized gold electrode

Radi, Abd-Elgawad; Muñoz-Berbel, Xavier; Cortina‐Puig, Montserrat; Marty, Jean‐Louis

doi:10.1016/j.electacta.2008.10.013

Cited by 74 publications

(45 citation statements)

References 39 publications

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“…In this paper, layers formed from the reductive adsorption of the 4-nitrophenyl diazonium salt followed by the electrochemical conversion of the nitro moiety to the amino moiety by both cyclic voltammetry (CV) and chronoamperometry (CA) are investigated, with the conversion verified using X-ray photoelectron spectroscopy (XPS). The electrochemical reduction of 4-nitrophenyl to 4-aminophenyl on gold has been studied by Harper et al [19,20] and Radi et al [21,22]. In both cases the reduction was performed by cyclic voltammetry in a protic medium.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Electrochemical Reduction of 4‐Nitrophenyl Covalently Grafted on Gold and Carbon

et al. 2010

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4-Nitrophenyl layers were grafted on gold and glassy carbon surfaces by electrochemical reductive adsorption of the corresponding diazonium salt. Electrochemical conversion efficiencies of 4-nitrophenyl moieties to 4-aminophenyl moieties on gold versus on glassy carbon in a protic medium were investigated using X-ray photoelectron spectroscopy (XPS). In total contrast to all previous comparative studies showing greater electrochemical reactivity of aryl diazonium salt-derived layers on gold than on glassy carbon, a much lower rate of conversion to 4-aminophenyl was observed on gold than on glassy carbon by both cyclic voltammetry (CV) and chronoamperometry (CA) methods. The lower electron transfer rate during conversion observed on gold versus glassy carbon was proposed to be due to a mechanism related to the molecular structure rearrangement of 4-nitrophenyl during the process on glassy carbon. However, whilst complete conversion of 4-nitrophenyl to 4-aminophenyl on gold by chronoamperometry was achieved, on glassy carbon complete reduction could not be achieved under the same conditions.

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Section: Introductionmentioning

confidence: 99%

A Comparative Study of Electrochemical Reduction of 4‐Nitrophenyl Covalently Grafted on Gold and Carbon

et al. 2010

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“…Крім того, застосування електродних сен-сорів включають визначення АФМ 1 в молоці [86,87], АФВ 1 в ячмені [88], АФВ 1 в зубчасто-му рисі [89], ОТА в пшениці [90] та Т-2 токси-ну в ячмені [91]. Перевагою електрохімічних випробувань є дешеве виробництво електро-дів; недоліками -дрейф сигналу і складність регенерації антитіл.…”

Section: застосування біосенсорів для моніторин-гу мікотоксинівunclassified

Application of Modern Biosensors Methods in Ecotoxicological Monitoring of Some Toxins of Natural (Micotoxins) and Antropogenic (Pesticides) Origin. Part 1. Micotoxins.

Гойстер¹,

Дзядевич²,

Мінченко³

2013

SEMST

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“…This feature corresponds to the typical electro-reduction reaction of the diazonium function, leading to the elimination of a nitrogen molecule and the production of highly reactive radicals. This radical was previously shown to attack the surface and to form a covalent bond between the aryl group and the Au electrode [39,40].…”

Section: Grafting Of P-nitrophenyl Diazonium Saltmentioning

confidence: 99%

“…Following deposition of the p-aminophenyl (p-AP) film on the gold electrode, the terminal amine was activated by incubation for 60 min with glutaraldehyde (25%) at pH 4, at room temperature [39]. The value of pH was chosen close to pKa of aminophenyl group.…”

Section: Procedures Of Covalent Grafting Of the Antibodymentioning

confidence: 99%

An Immunosensor for Pathogenic Staphylococcus aureus Based on Antibody Modified Aminophenyl-Au Electrode

Chrouda

Braiek

Rokbani

et al. 2013

ISRN Electrochemistry

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The objective of this work is to elaborate an immunosensing system which will detect and quantify Staphylococcus aureus bacteria. A gold electrode was modified by electrografting of 4-nitrophenyl diazonium, in situ synthesized in acidic aqueous solution. The immunosensor was fabricated by immobilizing affinity-purified polyclonal anti S. aureus antibodies on the modified gold electrode. Cyclic voltammetry (CV) and Faradaic Electrochemical Impedance Spectroscopy (EIS) were employed to characterize the stepwise assembly of the immunosensor. The performance of the developed immunosensor was evaluated by monitoring the electron-transfer resistance detected using Faradaic EIS. The experimental results indicated a linear relationship between the relative variation of the electron transfer resistance and the logarithmic value of S. aureus concentration, with a slope of 0.40 ± 0.08 per decade of concentration. A low quantification limit of 10 ± 2 CFU per ml and a linear range up to 10 7 ± 2 × 10 6 CFU per mL were obtained. The developed immunosensors showed high selectivity to Escherichia coli and Staphylococcus saprophyticus.

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An electrochemical immunosensor for ochratoxin A based on immobilization of antibodies on diazonium-functionalized gold electrode

Cited by 74 publications

References 39 publications

A Comparative Study of Electrochemical Reduction of 4‐Nitrophenyl Covalently Grafted on Gold and Carbon

A Comparative Study of Electrochemical Reduction of 4‐Nitrophenyl Covalently Grafted on Gold and Carbon

Application of Modern Biosensors Methods in Ecotoxicological Monitoring of Some Toxins of Natural (Micotoxins) and Antropogenic (Pesticides) Origin. Part 1. Micotoxins.

An Immunosensor for Pathogenic Staphylococcus aureus Based on Antibody Modified Aminophenyl-Au Electrode

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