An Electrochemical Nanosensor for Monitoring the Dynamics of Intracellular H₂O₂ Upon NADH Treatment

Abstract

Reactive oxygen species (ROS)-based therapeutic strategies play an important role in cancer treatment. However, in situ, real-time and quantitative analysis of intracellular ROS in cancer treatment for drug screening is still a challenge. Herein we report one selective hydrogen peroxide (H 2 O 2 ) electrochemical nanosensor, which is prepared by electrodeposition of Prussian blue (PB) and polyethylenedioxythiophene (PEDOT) onto carbon fiber nanoelectrode. With the nanosensor, we find that the level of intracellular H 2 O 2 increases with NADH treatment and that increase is dose-dependent to the concentration of NADH. Highdose of NADH (above 10 mM) can induce cell death and intratumoral injection of NADH is validated for inhibiting tumor growth in mice. This study highlights the potential of electrochemical nanosensor for tracking and understanding the role of H 2 O 2 in screening new anticancer drug.

Nanomaterials‐Induced Redox Imbalance: Challenged and Opportunities for Nanomaterials in Cancer Therapy

“…With this nanosensor, they find that the level of intracellular H 2 O 2 increases with NADH treatment, and intratumoral injection of high-dose of NADH can inhibit tumor growth in mice, providing a potential therapeutic strategy for cancer therapy. [353] Proposing the concept of hybrid bacteria, Sun et al conjugated Dox onto the surface of a non-pathogenic E. coli strain overexpressing glucose dehydrogenase through a stable linker of amide bonds. More amounts of NADPH are produced in tumor sites by glucose dehydrogenase within E. coli, promoting the generation of toxic ROS within the tumor.…”

Electrochemical Monitoring of Sphingosine-1-phosphate-Induced ATP Release Using a Microsensor Based on an Entropy-Driven Bipedal DNA Walker

“…Electrochemical microsensors with excellent temporal and spatial resolution have been widely employed for in situ monitoring of biomolecules in vivo. − For instance, a carbon fiber microelectrode (CFME) functionalized with DNA was fabricated to trace nitric oxide release from cells and tissues . Aptamer-based CFMEs were designed for highly selective probing of neurochemical dynamics in living systems .…”

Tri-mode Responses to Reactive Oxygen Species In Vivo by Chiral Vanadium-Based Nanoparticles

“…However, there is a paucity of high-sensitivity biological sensors because the associated preparation conditions are often complex, costly, and potentially toxic and may suffer from stability issues. Traditional techniques for monitoring ROS, such as electron paramagnetic resonance, fluorescence, − chemiluminescence, , and electrochemical analysis, − typically have several limitations, including poor stability, low sensitivity, and cumbersome operation . Therefore, it is of utmost importance to develop new methods with high sensitivity and superior biocompatibility to measure ROS in complex biological environments.…”