An Electrochemical Sensor Based on Simple Nano-Modification for Sensitive Detection of Ofloxacin Residue in Animal Derived Food

Abstract

The residue of ofloxacin (OFL) in animal-derived food would seriously affect human health. This study reports an inexpensive, fast, and simple electrochemical sensor for the sensitive detection of OFL in animal-derived food samples. A poly-L-glutamic acid/poly(sulfobutyl-β-cyclodextrin) CdTe quantum dot-modified electrode P(L-Glu)/SEB-β-CD@CdTeQDs/GCE was prepared by electrochemical polymerization reaction. We investigated the electrochemical behavior of OFL on the sensing electrode by cyclic voltammetry and square wave voltammetry. The results showed that the sensitivity of the sensor was significantly improved under the synergistic effect of L-glutamic acid, sulfobutyl-β-cyclodextrin, and CdTe quantum dot. In addition, the electrochemical reaction process of OFL on the sensing electrode was investigated and the results showed that the transfers of two protons and two electrons were involved in the reaction. Subsequently, the electrochemical sensing method based on P(L-Glu)/SEB-β-CD@CdTeQDs/GCE was developed and validated for the determination of OFL, showing a good linear response in the range of 0.07~1 μg·mL-1 with a detection limit of 3.4 ng·mL-1, and the precision and accuracy were high enough. Compared with the conventional HPLC method, the established electrochemical sensing method shows the merits of eco-friendly, shorter detection time, and much more simple, sensitive, and convenient.

A Novel Electrochemical Sensing Based on Cerium Oxide/Nitrogen-Doped Reduced Graphene Oxide for Sensitive Detection of Acetaminophen

“…18 Among them, electrochemical sensing detection methods have gradually gained widespread attention due to their high sensitivity, portability, rapidity, and low-cost advantages. 19 They have been applied in many fields such as environment, 20 medicine, 21 food, 22 and energy. 23 However, due to the effects of lower electrode kinetics and large overpotentials, 24 only modifying the electrode surface with the help of highly conductive electrocatalytic materials can improve the electron transfer rate and achieve rapid and sensitive detection of ACP.…”