An electrochemiluminescence biosensor for Kras mutations based on locked nucleic acid functionalized DNA walkers and hyperbranched rolling circle amplification

Zhang, Ying; Wang, Lixu; Luo, Fang; Qiu, Bin; Guo, Longhua; Weng, Zuquan; Lin, Zhenyu; Chen, Guonan

doi:10.1039/c7cc00009j

Cited by 76 publications

(23 citation statements)

References 27 publications

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“…To overcome these problems, this study aims to develop a novel electrochemical biosensor for coralyne assay using silver nanoparticles (AgNPs) as electrochemical probes . In order to improve the sensitivity and adaptability of DNA‐related sensing methods, amplification strategies are always integrated, such as polymerase chain reaction (PCR), rolling circle amplification (RCA), hybridization chain reaction (HCR), DNAzymes, and so on. Nucleases have also been widely applied for effective signal enhancement, owning to their specific catalytic properties in the engineering of DNA structures .…”

Section: Methodsmentioning

confidence: 99%

Exonuclease and Nicking Endonuclease‐Assisted Amplified Electrochemical Detection of Coralyne

Wang

Zhou

2017

ChemElectroChem

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Based on the catalysis of exonuclease I (Exo I) and nicking endonuclease (NEase), a novel electrochemical biosensor for the detection of coralyne is fabricated in this work. Specifically, DNA probe 1 can be protected from Exo I‐catalyzed digestion upon interacting with coralyne, the most representative homo‐adenine binding molecule. Subsequently, DNA probe 1 can further hybridize with a pendant sequence on top of the DNA tetrahedron, which is previously modified on the gold electrode surface. As the formed duplex contains the recognition site of Nb.BssSI, a type of NEase, the pendant sequence is thus cleaved, releasing the electrochemical probe on the 5’‐terminus from the electrode surface. DNA probe 1 is also released, which could then initiate the subsequent cycles of NEase‐catalyzed cleavage and amplify the declined electrochemical signals. We can determine coralyne in a linear range from 0.1 to 100 nM with the detection limit of 0.07 nM by using the proposed method. Experiments in real samples also demonstrate good results, showing excellent practical utility of this method. Moreover, it could be extended to assay other analytes by modifying certain DNA sequences.

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Section: Methodsmentioning

confidence: 99%

Exonuclease and Nicking Endonuclease‐Assisted Amplified Electrochemical Detection of Coralyne

Wang

Zhou

2017

ChemElectroChem

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“…As a powerful tool, a sensor has been employed to analyze chemical/biological molecules coupled with different detection methods, such as fluorescence, electrochemistry, electrochemiluminescence (ECL), colorimetry, surface enhanced Raman scattering (SERS) and surface plasmon resonance (SPR). To improve the analytical performance, many signal amplification strategies have been introduced into the construction of sensors, including DNA amplification technology, DNA walker, enzyme-assisted signal amplification and nanoprobes [ 1 , 2 , 3 , 4 , 5 ]. With the rapid development of nanomaterials, the nanoprobe has been considered as a promising signal amplification strategy to improve the performance of sensors.…”

Section: Introductionmentioning

confidence: 99%

Molybdenum Disulfide-Based Nanoprobes: Preparation and Sensing Application

et al. 2022

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The use of nanoprobes in sensors is a popular way to amplify their analytical performance. Coupled with two-dimensional nanomaterials, nanoprobes have been widely used to construct fluorescence, electrochemical, electrochemiluminescence (ECL), colorimetric, surface enhanced Raman scattering (SERS) and surface plasmon resonance (SPR) sensors for target molecules’ detection due to their extraordinary signal amplification effect. The MoS2 nanosheet is an emerging layered nanomaterial with excellent chemical and physical properties, which has been considered as an ideal supporting substrate to design nanoprobes for the construction of sensors. Herein, the development and application of molybdenum disulfide (MoS2)-based nanoprobes is reviewed. First, the preparation principle of MoS2-based nanoprobes was introduced. Second, the sensing application of MoS2-based nanoprobes was summarized. Finally, the prospect and challenge of MoS2-based nanoprobes in future were discussed.

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“…For example, gold nanoparticles participated colorimetric reaction, exists a situation where gold nanoparticles tend to aggregate which makes it difficult to reuse and extremely reduces the reproducibility of the assays( Chen et al 2014 ). In various ECL biosensing systems, tris (2,2’-bipyridyl)-ruthenium (II) (Ru(bpy) 3 2+ ) and its derivatives were commonly used for ECL emitters( Zhang et al 2017 ). For the fact that the Ru(bpy) 3 2+ ECL system consumes more emitting materials in the homogeneous solution, therefore, the stability of the system is affected, which greatly cuts down the detection efficiency and highly increases the cost.…”

Section: Introductionmentioning

confidence: 99%

Entropy-driven electrochemiluminescence ultra-sensitive detection strategy of NF-κB p50 as the regulator of cytokine storm

Zhang¹,

Fan²,

Yao

et al. 2021

Biosensors and Bioelectronics

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2019 novel coronavirus (2019-nCoV) with strong contagion in the crowd, has ravaged worldwide and severely impacts the human health and epidemic prevention system, by producing a series of significant stress reactions in the body to induce further cytokine storm. Transcription factors (TFs) served as essential DNA binding proteins play an integral role in regulating cytokine storm, and the detection of it in the human coronavirus environment provides especially valuable approaches to diagnosis and treatment of 2019-nCoV and development of antiviral drugs. In this work, an entropy-driven electrochemiluminescence (ECL) biosensor was constructed for ultra-sensitive bioassay of NF-κB p50. The strategy primarily capitalizing the splendid double-stranded DNA (dsDNA) binding properties of transcription factors, employing GOAu-Ru composite material as ECL emitter, utilizing entropy-driven reactions for signal amplification method, offered a repeatable proposal for TFs detection. In the absence of TFs, the released DNA1 further went in the entropy-driven reaction, contributing to an “ECL off” state. However, in the presence of TFs, the dsDNA avoided being digested, which blocked DNA1 for participating in the entropy-driven reaction, and the system exhibited an “ECL on” state. Most importantly, the ECL bioanalytical method denoted broad application prospects for NF-κB p50 detection with a lower detection limit (9.1 pM).

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An electrochemiluminescence biosensor for Kras mutations based on locked nucleic acid functionalized DNA walkers and hyperbranched rolling circle amplification

Cited by 76 publications

References 27 publications

Exonuclease and Nicking Endonuclease‐Assisted Amplified Electrochemical Detection of Coralyne

Exonuclease and Nicking Endonuclease‐Assisted Amplified Electrochemical Detection of Coralyne

Molybdenum Disulfide-Based Nanoprobes: Preparation and Sensing Application

Entropy-driven electrochemiluminescence ultra-sensitive detection strategy of NF-κB p50 as the regulator of cytokine storm

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