An Electrokinetically-Driven Microchip for Rapid Entrapment and Detection of Nanovesicles

Shi, Leilei; Esfandiari, Leyla

doi:10.3390/mi12010011

Cited by 18 publications

(24 citation statements)

References 56 publications

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“…(A) Schematic representation of a system for exosome trapping and characterization using DEP and EIS where triangular obstacles are used for electric field deformation and particle entrapment (Reprinted with permission from [40], © (2021) MDPI). (B) Comparison of the impedance of different particles in the same size range as a function of frequency (Reprinted with permission from [40], © (2021) MDPI). (C) Electrical characterization platform where exosomes are trapped in triangular obstacles with DEP (Reprinted with permission from [52], © (2019) American Chemical Society).…”

Section: Electrically Driven Microfluidic Tools For Exosome Manipulation and Characterizationmentioning

confidence: 99%

“…Shi et al. not only used pDEP to trap exosomes; they created a device able to individually detect these vesicles by using an AC voltage with a frequency ranging from 1 kHz to 10 MHz [40]—a range wider than that reported by Moore et al. (10 kHz–1.5 MHz) [52], but not as wide as that of Nicoliche et al.…”

Section: Electrically Driven Microfluidic Tools For Exosome Manipulation and Characterizationmentioning

confidence: 99%

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Electrically driven microfluidic platforms for exosome manipulation and characterization

et al. 2021

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Exosomes are small extracellular vesicles that can be obtained from several body fluids such as blood and urine. Since these vesicles can carry biomarkers and other cargo, they have application in healthcare diagnostics and therapeutics, such as liquid biopsies and drug delivery. Yet, their identification and separation from a sample remain challenging due to their high degree of heterogeneity and their co‐existence with other bioparticles. In this contribution, we review the state‐of‐the‐art on electrical techniques and methods to displace, selectively trap/isolate, and detect/characterize exosomes in microfluidic devices. Although there are many reviews focused on exosome separation using benchtop equipment, such as ultracentrifugation, there are limited reviews focusing on the use of electrical phenomena in microfluidic devices for exosome manipulation and detection. Here, we highlight contributions published during the past decade and present perspectives for this research field for the near future, outlining challenges to address in years to come.

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Section: Electrically Driven Microfluidic Tools For Exosome Manipulation and Characterizationmentioning

confidence: 99%

Section: Electrically Driven Microfluidic Tools For Exosome Manipulation and Characterizationmentioning

confidence: 99%

Electrically driven microfluidic platforms for exosome manipulation and characterization

et al. 2021

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“…The impedance signal was reported to be influenced by the concentration of entrapped particles [ 29 ]. This effect, to some extent, could be compensated by presenting the impedance signal as magnitude opacity, represented in Equation (1).…”

Section: Resultsmentioning

confidence: 99%

A Label-Free Electrical Impedance Spectroscopy for Detection of Clusters of Extracellular Vesicles Based on Their Unique Dielectric Properties

et al. 2022

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Extracellular vesicles (EVs) have gained considerable attention as vital circulating biomarkers since their structure and composition resemble the originating cells. The investigation of EVs’ biochemical and biophysical properties is of great importance to map them to their parental cells and to better understand their functionalities. In this study, a novel frequency-dependent impedance measurement system has been developed to characterize EVs based on their unique dielectric properties. The system is composed of an insulator-based dielectrophoretic (iDEP) device to entrap and immobilize a cluster of vesicles followed by utilizing electrical impedance spectroscopy (EIS) to measure their impedance at a wide frequency spectrum, aiming to analyze both their membrane and cytosolic charge-dependent contents. The EIS was initially utilized to detect nano-size vesicles with different biochemical compositions, including liposomes synthesized with different lipid compositions, as well as EVs and lipoproteins with similar biophysical properties but dissimilar biochemical properties. Moreover, EVs derived from the same parental cells but treated with different culture conditions were characterized to investigate the correlation of impedance changes with biochemical properties and functionality in terms of pro-inflammatory responses. The system also showed the ability to discriminate between EVs derived from different cellular origins as well as among size-sorted EVs harbored from the same cellular origin. This proof-of-concept approach is the first step towards utilizing EIS as a label-free, non-invasive, and rapid sensor for detection and characterization of pathogenic EVs and other nanovesicles in the future.

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“…Several techniques have been proposed for the extraction of EVs [18,28,29] and the addition of collected EVs to other cells to confirm their effects. However, such artificial administration of EVs cannot be performed in vivo.…”

Section: Discussionmentioning

confidence: 99%

Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate

et al. 2021

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Microfluidics is applied in biotechnology research via the creation of microfluidic channels and reaction vessels. Filters are considered to be able to simulate microfluidics. A typical example is the cell culture insert, which comprises two vessels connected by a filter. Cell culture inserts have been used for years to study cell-to-cell communication. These systems generally have a bucket-in-bucket structure and are hereafter referred to as a vertical-type co-culture plate (VTCP). However, VTCPs have several disadvantages, such as the inability to simultaneously observe samples in both containers and the inability of cell-to-cell communication through the filters at high cell densities. In this study, we developed a novel horizontal-type co-culture plate (HTCP) to overcome these disadvantages and confirm its performance. In addition, we clarified the migration characteristics of substances secreted from cells in horizontal co-culture vessels. It is generally assumed that less material is exchanged between the horizontal vessels. However, the extracellular vesicle (EV) transfer was found to be twice as high when using HTCP. Other merits include control of the degree of co-culture via the placement of cells. We believe that this novel HTCP container will facilitate research on cell-to-cell communication in various fields.

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An Electrokinetically-Driven Microchip for Rapid Entrapment and Detection of Nanovesicles

Cited by 18 publications

References 56 publications

Electrically driven microfluidic platforms for exosome manipulation and characterization

Electrically driven microfluidic platforms for exosome manipulation and characterization

A Label-Free Electrical Impedance Spectroscopy for Detection of Clusters of Extracellular Vesicles Based on Their Unique Dielectric Properties

Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture Plate

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