An electrophoretic and ultracentrifugal study of boar seminal plasma

Boursnell, J. C.; Johnson, Patricia A.; Zamora, Jorge

doi:10.1016/0006-3002(62)90101-4

Cited by 26 publications

(26 citation statements)

References 9 publications

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“…In boar seminal plasma, cationic proteins are reported to be the major constituent (Boursnell et al, 1962). These basic proteins increase the membrane permeability (Hibbitt and Benians, 1971).…”

Section: Introductionmentioning

confidence: 99%

Anionic and cationic components from protein aggregates in bovine seminal plasma and their effects of sperm motility

al-Somai

Vishwanath

Molan

et al. 1994

Molecular Reproduction Devel

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Bovine seminal plasma proteins are in an aggregated form of high molecular weight in their native state. By immobilisation on a cation exchanger with exposure to disaggregating conditions (i.e., acetonitrile and low pH), the high-molecular-weight aggregates could be dissociated to slowly release the low-molecular-weight components. The anionic component released from the cation exchanger during disaggregation was collected by adsorption on a hydrophobic interaction column. The cationic component remaining on the cation exchanger was eluted with NaOH. Both components were found on gel permeation chromatography to be < 5 kDa. SDS-PAGE of the various fractions showed that components of low molecular weight were still in an aggregated form. These components resulting from the disaggregation process have detrimental effects on sperm motility and the effects were more substantial compared with that of whole seminal plasma. All the cationic components were significantly detrimental to sperm motility, especially the fractions of low molecular weight. The anionic fractions reduced sperm motility when in an aggregated state. The isolated anionic peptide was not detrimental in its free form. In all fractions the peptides tended to re-aggregate to a higher molecular weight under neutral conditions, however, the isolated anionic peptide (molecular weight < 1,500) failed to do so.

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Section: Introductionmentioning

confidence: 99%

Anionic and cationic components from protein aggregates in bovine seminal plasma and their effects of sperm motility

al-Somai

Vishwanath

Molan

et al. 1994

Molecular Reproduction Devel

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“…The majority of the protein components in the seminal plasma of the boar are secreted by the seminal vesicles (Boursnell, Johnson & Zamora, 1962). Three principal components are involved, two of which, A and H, are positively charged and have isoelectric points of 8-8 and 9-4, respectively (Boursnell & Briggs, 1969).…”

Section: Introductionmentioning

confidence: 99%

The Removal of the Seminal Vesicles From the Boar and the Effects on the Semen Characteristics

Davies¹,

Hall²,

Hibbitt³

et al. 1975

Reproduction

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A technique is described for the removal of the seminal vesicles from the boar. The operation was carried out on twelve animals and six of the animals were subsequently trained for semen collection. The seminal plasma from the boars after surgery compared with normal litter mates had a more watery consistency and did not form the characteristic gelduring ejaculation. The sperm concentration was 49% lower while the total reduction of sperm number ejaculate was 78% in the experimental animals, but the ratio of living to dead spermatozoa remained unchanged. The concentrations of citrate and protein were significantly depressed in the seminal plasma of the animals after surgery and the pH increased; the osmolarity remained unchanged. In semination of gilts with the semen from experimental boars revealed no significant loss of fertility compared with the normal controls. Animals slaughtered up to 17 months after surgery showed no regeneration of the seminal vesicles.

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“…In most of the early studies of the biochemical characteristics of seminal plasma, attention was focussed mainly on the relative contributions of the different areas of the male reproductive tract. For example, boar seminal plasma proteins are predominantly basic and originate mainly from the seminal vesicles (2,3,5,17). By their strong binding capacity to the plasma membrane (HIBBITT and BENIANS, ll), these basic proteins increase the permeability of the sperm plasma membrane, cause severe loss of enzymes from the spermatozoa (MOORE et al, 23) and thus render the spermatozoa susceptible to cold shock (23).…”

Section: Introductionmentioning

confidence: 99%

Some Biochemical Characteristics of Porcine Seminal Plasma under Stressful Hot Humid Climatic Conditions

Egbunike

Jeyakumar

2010

Zentralblatt Für Veterinärmedizin Reihe A

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Summary Eight adult heat‐adapted Large White boars raised and maintained in the humid tropics were ejaculated at 48‐hourly intervals for three weeks, after which four of them were subjected to direct heat stress by exposure to direct tropical sunlight in the paddocks. Semen collection continued during this exposure and for another three weeks in all boars. The seminal plasma protein content was similar in all boars but the protein profile was different between heat‐stressed (NS) and shaded (S) boars. In the NS boars, the proportion of basic albumin‐like proteins increased, while gamma globulin‐type proteins decreased in the week following heat stress. The proteins with the same electrophoretic mobility as α1 globulin increased in the second and third weeks after treatment. Lipid phosphorus and fructose levels remained unchanged, unlike cholesterol, which increased in the second week post‐exposure. It is concluded that, immediately after heat stress, boar semen is unsuitable for preservation because of its increased basic nature. Zusammenfassung Biochemische Änderungen des Samenplasmas von Ebern unter Streß des feuchtheißen Klimas Bei 8 adulten Ebern der weißen Rasse, aufgezogen im feuchten Tropenklima, wurden während 3 Wochen jeden 2. Tag Spermaproben entnommen. Anschließend wurden 4 Eber täglich einem Hitzestreß (3 Tage direkte Sonnenbestrahlung im Auslauf während 45 min täglich) ausgesetzt. Bei alien Ebern wurden während weiteren 3 Wochen Spermaproben entnommen. Der Proteingehalt des Samenplasmas war bei alien Ebern ähnlich, hingegen zeigte das Proteinspektrum der sonnenbestrahlten Eber im Vergleich zu den im Schatten gehaltenen Tieren Unterschiede. Bei den bestrahlten Ebern erhöhten sich in der Woche nach dem Hitzestreß die basischen albuminartigen Proteine, während die Proteine des γ‐Globulin‐Typs abnahmen. Proteine mit der gleichen elektrophoretischen Wanderung wie das α1‐Globulin erhöhten sich in der zweiten und dritten Woche nach dem Streß. Die Konzentration der Phosphorlipide und der Fructose veränderten sich nicht im Gegensatz zur Cholesterolkonzentration, die sich in der zweiten Woche nach der Hitzeexposition erhöhte. Folgerung: Unmittelbar nach einem Hitzestreß eignet sich Ebersamen wegen seines erhöhten basischen Charakters nicht für die Konservierung. Résumé Modifications biochimiques du plasma du sperme chez des verrats soumis au stress d'un climat chaud et humide Des échantillons de spermes ont été prélevés tous les 2 jours durant 3 semaines chez 8 verrats adultes de la race porc blanc élevés dans un climat tropical humide. 4 verrats furent exposés à un stress de chaleur quotidien (3 jours d'exposition directe aux rayons du soleil durant 45 minutes lors de leur sortie). Des échantillons des spermes furent encore prélevés pendant 3 semaines. Le taux de protéines du plasma du sperme fut semblable chez tous les verrats, par contre le spectre protéinique des verrats exposés au soleil montra des differences par rapport à ceux maintenus à l'ombre. Les protéines basiques du genre albumine augmentèrent dan...

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An electrophoretic and ultracentrifugal study of boar seminal plasma

Cited by 26 publications

References 9 publications

Anionic and cationic components from protein aggregates in bovine seminal plasma and their effects of sperm motility

Anionic and cationic components from protein aggregates in bovine seminal plasma and their effects of sperm motility

The Removal of the Seminal Vesicles From the Boar and the Effects on the Semen Characteristics

Some Biochemical Characteristics of Porcine Seminal Plasma under Stressful Hot Humid Climatic Conditions

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