An electrophoretic comparison of enzymes of ballistosporogenous yeasts.

Abstract

The patterns produced by nine enzymes of ballistosporogenous yeasts and supposedly related yeasts were studied by electrophoresis on polyacrylamide gel. Forty-two strains belonged to the genera Sporobolomyces, Sporidiobolus, Aessosporon and Bullera and twenty-four strains to Rhodotorula, Rhodosporidium, Cryptococcus, unidentified ballistosporogenous yeasts, and Candida edax. Four Sporobolomyces salmonicolor strains, two Sporobolomyces holsaticus strains, two Sporobolomyces odorus strains, five Sporidiobolus salmonicolor strains, two Aessosporon salmonicolor strains, and one strain of Aessosporon dendrophilum produced similar electrophoretic patterns. Moreover, mating was observed between some of these strains. Sporobolomyces roseus and Sporobolomyces shibatanus differed from Sporobolomyces salmonicolor in their glucose-6-phosphate dehydrogenase (EC 1.1.1.49.), but the patterns of the other enzymes were similar; all three species differed clearly from Sporobolomyces singularis, Sporobolomyces gracilis, Sporobolomyces puniceus, and Sporobolomyces antarcticus. Sporobolomyces albo-rubescens showed a peculiar 6-phosphogluconate dehydrogenase (EC 1.1.1.41.) pattern and was similar to two Rhodotorula rubra strains in the pattern of their enzymes. Close relationships were also seen between Sporidiobolus ruinenii and Rhodotorula graminis, and between Bullera alba and a strain in Cryptococcus albidus var. albidus in the electrophoretic patterns of their enzymes. Four unidentified strains which had lost the ability to produce ballistospores had patterns similar to those of Bullera alba, Rhodotorula glutinis, Cryptococcus laurentii var. flavescens, and Cryptococcus macerans. Three colorless strains, putatively derived from a strain of Sporobolomyces roseus, showed the same electrophoretic patterns as the strains from which they originated.

Value of chemosystematic data for predicting anamorph-teleomorph relationships between the genera Rhodotorula and Rhodosporidium

“…In such a case, identical chemosystematic data, particularly high values of DNA-relatedness, at the molecular level will support strongly the anamorph-telomorph relationship between yeast species. This procedure is useful for other yeasts species, and the electrophoretic comparison of enzymes has been applied to the study of ballis-507 tosporogenous yeasts [21] and ascomycetous yeasts [22].…”

Sporobolomyces griseoflavus and Sporobolomyces sasicola, two new species of ballistosporous yeasts isolated from dead leaves of Sasa sp. in Japan.

“…Isolation of colorless mutants of Sporobolomyces has been reported by several investigators (1,12,13). YAMAZAKI and KOMAGATA (14) confirmed by electrophoretic comparison of enzymes that some colorless strains of ballistosporous yeasts were colorless mutants of typical Sporobolomyces species such as Sporobolomyces roseus. However, no species corresponding to Sp.…”

“…(Received January 14,1987) Two new species of ballistospore-forming yeasts, Sporobolomyces griseoflavus and Sporobolomyces sasicola, were isolated from dead leaves of Sasa sp. collected on a mountain in Japan.…”

Development of resources for the analysis of gene function in Pucciniomycotina red yeasts

“…The strain was originally isolated from a willow tree leaf in Japan (Yamazaki and Komagata, 1983). IAM 13481 has been renamed Sporobolomyces sp.…”