An electrophoretic investigation of interactions between bovine plasma albumin and charged dextran derivatives

Abstract

The formation of soluble complexes through the interactions of macromolecules bearing net charges of the same sign is a phenomenon of considerable interest. Particular examples of this type of complex may be found in the antigen-antibody system (e.g. Singer & Campbell, 1955a, b, c; Singer, Eggman & Campbell, 1955), the polymerization of acid insulin (Oncley, Ellenbogen, Gitlin & Gurd, 1952; Doty & Myers, 1953), the polymerization of oc-chymotrypsin (Kegeles & Rao, 1958), the interaction between nucleic acids and proteins (Goldwasser & Putnam, 1950) and the interactions of plasma proteins with charged polysaccharides (Walton, 1952; Noguchi, 1956; Bernfeld, Donahue & Berkowitz, 1957; Sasaki & Noguchi, 1959). The work reported in this paper is an investigation, with free-boundary electrophoresis, of the effect of variations of pH, ionic strength and molecular size on the formation of soluble complexes between bovine plasma albumin and synthetic derivatives of dextran. Two types of dextran derivatives have been employed: a sulphate ester and an ether-linked diethylaminoethyl derivative. Interactions between the bovine plasma albumin

The interaction of collagen and acid mucopolysaccharides. A model for connective tissue

“…Nature of the interaction. The appearance of a different number of boundaries in the two limbs of the electrophoresis cell appears to be a common phenomenon and has been reported also for mixtures of ovomucoid with nucleic acid (Longsworth & Maclines, 1942), for serum albumin with heparin (Chargaff, Ziff & Moore, 1941), with chondroitin sulphate (Gorter & Nanninga, 1952) or with sulphated dextran (Thompson & McKernan, 1961), and for lipoprotein with heparin (Bernfeld et al 1957). Although a general theory for interpretation of moving-boundary patterns in interacting systems that re-equilibrate is not available, a limited theory for such a model system has been presented for certain special cases by Gilbert & Jenkins (1959).…”

Extent of Charge Screening in Aqueous Polysaccharide Solutions

“…Weight-concentrations of the dialyzed polysaccharide solutions c P α (mg/mL) were obtained refractometrically by means of the expression where J is the number of Rayleigh interference fringes observed in a synthetic boundary run on the dialyzed solution of polysaccharide with specific refractive index d n /d c (mL/g). Values used for the various polysaccharides were the following: heparin, 0.132 mL/g; dextran sulfate, 0.114 mL/g; polygalacturonate, 0.155 mL/g …”