An elevated glutathione S-transferase in creosote-resistant mummichog (Fundulus heteroclitus)

Armknecht, Susan; Kaattari, Stephen L.; Veld, Peter A. Van

doi:10.1016/s0166-445x(97)00074-x

Cited by 48 publications

(29 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vertebrates, induction of specific GST following exposure to diverse agents such as PAHs, PCBs and organochloride pesticides has been well documented (Willett et al, 1997;Armknecht et al, 1998). Although metals are not natural substrata for these enzymes, recent studies have shown in vivo GST induction in fish exposed to heavy metals (Paris-Palacios et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

The Use of Mytilus Galloprovincialis Acetylcholinesterase and Glutathione S-Transferases Activities as Biomarkers of Environmental Contamination Along the Northwest Portuguese Coast

Moreira

Guilhermino

2005

Environ Monit Assess

View full text Add to dashboard Cite

With the aim of using Mytilus galloprovincialis acetylcholinesterase (AChE) and glutathione S-transferases (GST) activities as biomarkers of environmental contamination in risk assessment studies along the northwest Portuguese coast, the objective of this study was to provide background information related to: (1) baseline values of these enzymatic activities both in reference and contaminated areas and their responsiveness as indicators of exposure to environmental contaminants; (2) the importance of seasonal variations of such biomarkers in M. galloprovincialis along this area. For this, the activity of these enzymes was seasonally determined in mussels collected from four sites, including a reference and three stations with different contamination sources along the referred area. Statistically significant differences on both enzymatic activities were found among the four sampling stations, at the four sampling periods. In comparison to the reference station, lower AChE and higher GST activity values were found in mussels collected in stations potentially contaminated by pesticides and domestic/industrial effluents and in mussels collected in the vicinity of an oil refinery and an industrial/mercantile harbour, respectively. The results obtained in this work highlighted the potential suitability of these biomarkers to be used as components of environmental monitoring programs in risk assessment studies along the northwest Portuguese coast. Since a seasonal variation in both enzymatic activities was found, the possible implications of such variability in the use of these enzymes as environmental biomarkers are also discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

The Use of Mytilus Galloprovincialis Acetylcholinesterase and Glutathione S-Transferases Activities as Biomarkers of Environmental Contamination Along the Northwest Portuguese Coast

Moreira

Guilhermino

2005

Environ Monit Assess

View full text Add to dashboard Cite

show abstract

“…In vertebrates, induction of specific GSTs following exposure to diverse agents such as polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) has been well documented (Armknecht et al, 1998;Stien et al, 1998;Willett et al, 1997). However, conflicting results have been reported regarding GSTs induction by these sorts of contaminants in mussels, particularly following exposure to PAH-type compounds (Akcha et al, 2000;Michel et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

The ?Coral Bulker? Fuel Oil Spill on the North Coast of Portugal: Spatial and Temporal Biomarker Responses in Mytilus galloprovincialis

et al. 2004

View full text Add to dashboard Cite

Abstract. In December 2000, the ship 'Coral Bulker' ran aground at the entrance of the port of Viana do Castelo (North-west coast of Portugal). A large amount of fuel oil was spilled and part of it reached the shore. To evaluate the spatial and temporal impact of this oil spill, a field study, and several laboratory toxicity tests were performed using Mytilus galloprovincialis as biological indicator of environmental contamination and the biomarkers glutathione S-transferases (GSTs) and acetylcholinesterase (AChE) as indicative criteria. Fifteen days after the oil spill, mussels collected at stations located near the ship presented higher and lower values of GSTs and AChE activity, respectively. These results, and those obtained in the laboratory toxicity tests, evidence that these biomarkers were sensitive indicators of exposure to this kind of pollution and were able to monitor a spatial impact of the oil spill of at least 10 km, confirming the higher level of contamination near the ship and a contamination gradient along the sampling stations. One year after the accident, such a contamination gradient was no longer evident. This study highlight the potential suitability of a biomarker approach for assessing spatial and temporal impacts of marine pollution accidents, such as fuel oil spills, suggesting the inclusion of these biomarkers in risk assessment studies, as cost-effective and early warning recognized tools. Major advantages and limitations of the biomarker approach used in this study are further discussed.

show abstract

“…Glutathione-S-transferase (GST) is a phase II enzyme that can successfully conjugate toxic PAH metabolites [35] and eliminate ROS through conjugation to glutathione. Killifish from sites contaminated by PAHs (e.g., Elizabeth River) and PCBs, dioxins and PAHs (NB) had elevated expression and activity of GST relative to reference-site animals [36,37]. Bello et al [7] reported that GST induction by AHR ligands is greater in resistant NBH killifish than in reference-site animals.…”

Section: Killifish Populations Heart Deformities Indexmentioning

confidence: 99%

Polluted‐site killifish (Fundulus heteroclitus) embryos are resistant to organic pollutant‐mediated induction of CYP1A activity, reactive oxygen species, and heart deformities

Arzuaga

Elskus

2010

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Exposure to coplanar polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) can adversely affect fish embryonic development, induce expression of cytochrome P4501A (CYP1A), and increase reactive oxygen species (ROS) production, effects believed to be mediated by the aryl hydrocarbon receptor (AHR). Killifish (Fundulus heteroclitus) populations in New Bedford Harbor, Massachusetts, USA (NBH) and Newark Bay, New Jersey, USA (NB) are generationally exposed to coplanar PCBs and PAHs and have developed resistance to PCB mediated induction of CYP1A. We hypothesized that fish resistant to CYP1A induction would also exhibit resistance to PCB and PAH induced ROS production and teratogenesis. Killifish embryos from two contaminated (NB, NBH) and two reference-site populations were exposed to vehicle or 3,3'4,4'5-pentachlorobiphenyl (PCB126) or 3-methylcholanthrene (3-MC) and evaluated for in ovo CYP1A activity, heart deformities, and ROS production. Both chemicals significantly increased in ovo ethoxyresorufin-O-deethylase (EROD) and ROS production in reference-site embryos. These chemicals provoked only moderate induction of in ovo EROD in NBH and NB embryos, and neither PCB126 nor 3-MC induced ROS production in these populations. Similarly, heart deformities were significantly induced by PCB126 in reference-site embryos, but had no significant effects on NB and NBH animals. These results indicate that fish resistant to CYP1A induction also exhibit decreased sensitivity to PCB126 and 3-MC-induced ROS production and teratogenesis. These findings further our understanding of toxicant resistance by demonstrating that reduced response to coplanar PCBs and PAHs extends beyond resistance to CYP1A induction to resistance to the physiological and teratogenic effects of these toxicants, responses that undoubtedly contribute to the increased survival of killifish inhabiting contaminated sites.

show abstract

An elevated glutathione S-transferase in creosote-resistant mummichog (Fundulus heteroclitus)

Cited by 48 publications

References 48 publications

The Use of Mytilus Galloprovincialis Acetylcholinesterase and Glutathione S-Transferases Activities as Biomarkers of Environmental Contamination Along the Northwest Portuguese Coast

The Use of Mytilus Galloprovincialis Acetylcholinesterase and Glutathione S-Transferases Activities as Biomarkers of Environmental Contamination Along the Northwest Portuguese Coast

The ?Coral Bulker? Fuel Oil Spill on the North Coast of Portugal: Spatial and Temporal Biomarker Responses in Mytilus galloprovincialis

Polluted‐site killifish (Fundulus heteroclitus) embryos are resistant to organic pollutant‐mediated induction of CYP1A activity, reactive oxygen species, and heart deformities

Contact Info

Product

Resources

About