This review chapter is dedicated to multichannel audio source separation in real-life environment. We explore some of the major achievements in the field and discuss some of the remaining challenges. We will explore several important practical scenarios, e.g. moving sources and/or microphones, varying number of sources and sensors, high reverberation levels, spatially diffuse sources, and synchronization problems. Several applications such as smart assistants, cellular phones, hearing aids and robots, will be discussed. Our perspectives on the future of the field will be given as concluding remarks of this chapter.