The deactivation of Earth Observing-1 satellite has resulted in the termination of capturing Hyperion data as a unique source of hyperspectral satellite imagery. These images also were collected through an on-demand service and thereby are not available for the entire Earth's surface. The Operational Land Imager (OLI) sensor, on the other hand, provides a free source of multi-spectral images with global coverage. Recognized these facts, the aim of this paper is to enhance the spectral resolution of OLI images by using existing Hyperion imageries to generate a high spectral resolution image for a desired date and site. This was conducted through the artificial neural network (ANN). To find the suitable ANN, we compared the performance of multilayer perceptron (MLP) and radial basis function (RBF) networks for spectral enhancement.The research obtained two Hyperion and OLI images covering West Region of Tehran, Iran, on 4 January 2016. From 242 original Hyperion spectral bands, we selected 31 bands to reproduce from OLI spectral bands. These were determined through visual inspection, principal component analysis and Pearson's correlation test. The MLP and RBF networks were generated based on the OLI bands 1-7 and per 31 Hyperion bands as input and output layers respectively. The comparison between the spectral bands of spectra-enhanced image and original Hyperion data indicated a good agreement (0.884 > R 2 > 0.692). This study also found MLP network delivered higher accuracy against RBF network for spectral enhancement. The spectra-enhanced image can be used in studies with the need of images with continuous spectral bands.