2013
DOI: 10.1039/c3lc41419a
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An enclosed in-gel PCR amplification cassette with multi-target, multi-sample detection for platform molecular diagnostics

Abstract: This work describes a self-contained, simple, disposable, and inexpensive gel capillary cassette for DNA amplification in near point of care settings. The cassette avoids the need for pumps or valves during raw sample delivery or polymerase chain reaction (PCR) amplification steps. The cassette contains capillary reaction units that can be stored at room temperature for up to 3 months. The current cassette configuration format simultaneously tests up to 16 patients for two or more targets, accommodates differe… Show more

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Cited by 22 publications
(56 citation statements)
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“…Another interesting concept for molecular diagnostics supporting different type of samples utilizes modular in-gel PCR amplification cassettes configurable for multi-target detection (Figure 3e) (Manage et al, 2013). Modularity is achieved by isolating the detection elements, which consist of 7 mm-long capillary tubes filed with the PCR reaction mix and gel reagents.…”
Section: Sample Preparationmentioning
confidence: 99%
“…Another interesting concept for molecular diagnostics supporting different type of samples utilizes modular in-gel PCR amplification cassettes configurable for multi-target detection (Figure 3e) (Manage et al, 2013). Modularity is achieved by isolating the detection elements, which consist of 7 mm-long capillary tubes filed with the PCR reaction mix and gel reagents.…”
Section: Sample Preparationmentioning
confidence: 99%
“…In this method RCA starts in the presence of circular DNA and other reaction components. The detection of the RCA products can be performed using surface associated methods such as microbead assisted diffractometry detection [78] and surface enhanced Raman spectroscopy [72]. Thus single protein recognition events are amplified thousands of times reaching a system sensitivity of 10 zeptomolar (1000 protein molecules) [72].…”
Section: Isothermal Amplification On Microarraysmentioning
confidence: 99%
“…The rapidly growing field of soft-lithography driven microfluidic technology has resulted in the development a plethora of micro-devices for extraction [75] and amplification and analysis [31,[76][77][78][79][80][81] of nucleic acids. The main idea of microarray technology, namely spatial separation of microarray elements and their simultaneous subjection to one sample of interest can be easily realized using highly parallel architectures of micrometresized channels available/affordable due to microfluidics.…”
Section: Microfluidic Arraysmentioning
confidence: 99%
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“…In general, potential applications for microchips have been demonstrated through various analytes, including viruses, 10,11 disease biomarkers, [12][13][14] environmental pollutants, [15][16][17] proteins and enzymes, [18][19][20][21][22] mammalian cells, [23][24][25] and antibiotics. 26 These involve various bioanalytical assays such as hybridization, immunoassay, and cell/viral analysis.…”
mentioning
confidence: 99%