An endogenous retrovirus mediating deletion of αβ T cells?

Woodland, David L.; Happ, Mary Pat; Gollob, Kenneth J.; Palmer, Ed

doi:10.1038/349529a0

Cited by 265 publications

(106 citation statements)

References 28 publications

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“…The role of H-2 k product(s) was confirmed by the lack of V~20 expression in the congenic B6.H-2L The strain distribution of V320 expression follows that observed for V~lTa1 and V311, which are controlled in I-Epositive mice by two kinds of self superantigens: a nonpolymorphic B cell-specific product of an unknown nature mediates the deletion of V317al-bearing T cells (13,14), and integrated sequences related to mouse mammary tumor virus prevent V1311 expression (15)(16)(17) specificity toward deleting elements are located in a loop distant from the site of interaction with MHC/antigenic peptide complex (18). Minor alterations in this region of VB17a1 drastically alter its reactivity toward Mls2/3 (10).…”

Section: Expression Of V320 Studies Of V3 Usage By Stainings Withmentioning

confidence: 92%

“…VB20 was amplified with KgDO (complementary to positions 472-488 of V1520) and XNSC10. V~20-specific PCtLs were done with KguP (positions 241-256 of VB20) and MTB primers, of TCR B transcripts with the MCTBUP (positions [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] and an equimolar mixture of the MTB1DO and MTB2DO primers (complementary to positions 474-493 of C131 and C132), of TCR c~ transcripts with MTCAUP (positions [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] and MTCADO2 primers (complementary to positions 263-279), and of V1317 with MVB17S and MVB17FX, as previously described (10).…”

Section: Methodsmentioning

confidence: 99%

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Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice.

Six

Jouvin‐Marche

Loh

et al. 1991

The Journal of Experimental Medicine

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SummaryA cDNA library of TCR/3 chain transcripts from BALB/c thymocytes was constructed using anchored polymerase chain reaction (PCR). Screening of this library led to the identification of a VB gene segment, V/320, structurally related to V/33 and V1317. Genomic analysis of mice displaying deletions in their V/3 loci, together with mapping of cosmid clones, situated V/320 2.5 kb beside VB17. The expression of VB20 was estimated by PCR in mice of different H-2 and Mls types. Peripheral T cells from H-2 k and H-2 d mice did not express V/320, whereas in I-E-negative mice (C57B1/6 and SJL), V/320 transcripts were detected. The lack of V/320 transcripts in (C57B1/6 x CBA/J)F1, (C57B1/6 x BALB/c)F1, and in congenic B6.H-2 k mice suggests that the differential use of V/320 is due to an I-E-mediated donal deletion process. The involvement of the Mls super antigens was excluded by analysis of all Mls type combinations. The nature of the V/B20-deleting element(s) is discussed in the context of the I-EAuperantigen systems controlling the expression of V/311 and VB17.

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Section: Expression Of V320 Studies Of V3 Usage By Stainings Withmentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice.

Six

Jouvin‐Marche

Loh

et al. 1991

The Journal of Experimental Medicine

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“…Furthermore, it has recently been demonstrated that endogenous Mtv retroviral sequences are homologous to Mls (minor lymphocyte stimulatory) superantigens and thus serve to alter the T cell repertoire (54)(55)(56)(57) …”

Section: Resultsmentioning

confidence: 99%

Beta cell expression of endogenous xenotropic retrovirus distinguishes diabetes-susceptible NOD/Lt from resistant NON/Lt mice.

et al. 1992

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“…These are proteins that stimulate T cells bearing the appropriate V13 element almost independently ofcontributions from other parts of the TCRs borne by the T cells (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22). Such proteins, called superantigens because they stimulate large numbers ofT cells, are encoded by microorganisms including retroviruses, bacteria, and mycoplasma (22)(23)(24)(25)(26)(27)(28)(29).…”

mentioning

confidence: 99%

A method for production of antibodies to human T-cell receptor beta-chain variable regions.

Choi

Kotzin

Lafferty

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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Mouse T-cell hybridomas bearing human Vp elements were produced by transfection of human/mouse hybrid T-cell receptor 18-chain genes into a mouse T-cell hybridoma lacking an endogenous #-chain gene. These hybridomas were entirely mouse in origin except for the human Vp region. These cells were used to immunize mice against human V13 elements. Mouse monoclonal antibodies have thus been generated against human Vp13.1 and -13.2. We expect that the method outlined in this paper will be useful in the production of monoclonal antibodies specific for other human V13 or V.elements.The receptor for antigen on most peripheral T cells is made of a and 13 chains. Usually these T-cell receptors (TCRs) engage antigen in the form of peptides, derived from the antigen, bound to cell surface major histocompatibility complex (MHC) molecules (1-4). The specificity of the TCR for this complex ligand is determined by the five variable elements (Va, Ja, V13, Do, and JO3) of the a and 13 chains as well as non-germ-line-encoded junctional regions (5-8). Exceptions to this rule have been documented by us and others. These are proteins that stimulate T cells bearing the appropriate V13 element almost independently ofcontributions from other parts of the TCRs borne by the T cells (9-22). Such proteins, called superantigens because they stimulate large numbers ofT cells, are encoded by microorganisms including retroviruses, bacteria, and mycoplasma (22-29).There is considerable interest in the control and consequences of the T-cell repertoire in humans and other species. Obviously a detailed understanding of the T-cell repertoire would require knowledge of all the V,, Ja, V13, D13, and Jo combinations present in a given individual. Such knowledge will not be available in the foreseeable future. However, much has already been learned from studies of the distribution between animals of just one of the TCR variable elements, V13. Examination of V13 expression in mice has been used to study positive selection, T-cell tolerance, autoimmunity, and the consequences of attack by superantigens (22).Similarly, V13 expression in humans has been used to study the effects of superantigens and also to examine the repertoires of T cells involved in inflammatory and autoimmune disease (18,19,29,30 MATERIALS AND METHODSPreparation of RNA and cDNA and Amplification by QPCR. Total RNA was prepared from phytohemagglutinin-, antihV,013

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An endogenous retrovirus mediating deletion of αβ T cells?

Cited by 265 publications

References 28 publications

Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice.

Identification of a T cell receptor beta chain variable region, V beta 20, that is differentially expressed in various strains of mice.

Beta cell expression of endogenous xenotropic retrovirus distinguishes diabetes-susceptible NOD/Lt from resistant NON/Lt mice.

A method for production of antibodies to human T-cell receptor beta-chain variable regions.

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