An endophytic strain JK of genus bacillus isolated from the seeds of super hybrid rice (Oryza sativa L., Shenliangyou 5814) has antagonistic activity against rice blast pathogen

Jing, Ruixue; Wang, Weiping; Liu, Yang

doi:10.1016/j.micpath.2020.104422

Cited by 22 publications

(6 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacillus and Priestia endophytes colonize different organs in rice, such as the root, stem, leaves, and grain, showing a great diversity of species and participating as promoters of plant growth [ 14 ] and in the degradation of agrochemicals [ 26 ], suppression of diseases [ 15 , 27 ], and induced systemic resistance [ 28 ]. The genus Burkholderia has been reported as a frequent endophyte in rice at the root level [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Co-Inoculation of Endophytes Bacillus siamensis TUR07-02b and Priestia megaterium SMBH14-02 Promotes Growth in Rice with Low Doses of Nitrogen Fertilizer

Ríos-Ruiz

Tuanama-Reátegui

Huamán-Córdova

et al. 2023

Plants

View full text Add to dashboard Cite

Multiple biotic and abiotic factors influence rice cultivation. These factors limit productivity and yield, as well as an irrational use of agrochemicals in rice cultivation. A sustainable alternative is using selected growth-promoting microorganisms to increase nutritional efficiency. In the present study, the direct mechanisms of growth promotion in two strains of Bacillus, three strains of Priestia, and two strains of Burkholderia endophytes of rice were characterized. Bacillus siamensis TUR07-02b and Priestia megaterium SMBH14-02 were selected to promote Oryza sativa var’s growth. “Bellavista” was used at different doses (50, 75, and 100%) of mineral nitrogen (N) using a randomized block design by quintuplicate. Both strains, SMBH14-02 and TUR07-02b, presented outstanding promoter characteristics, including auxin production (123.17 and 335.65 μg mL−1, respectively) and biological nitrogen fixation capacity. Similarly, B. siamensis TUR07-02b could solubilize phosphate-Ca (20.94 μg mL−1), cellulases, and pectinases. Under greenhouse conditions, co-inoculated plants receiving 75% of the total dose of mineral nitrogen showed increased agronomic parameters in relation to panicle length, grains per panicle, grain yield, and harvest index by 25.0, 30.7, 39.5, and 12.5%, respectively, compared to the 75% fertilized treatment without inoculation. The strains of B. siamensis TUR07-02b and P. megaterium SMBH14-02 are potential microbial resources in the formulation of new inoculants to reduce the use of nitrogenous fertilizers. Thus, agronomic validation of the inoculant consortium at the field level will be an essential step in providing an alternative for the sustainable management of rice cultivation and increased productivity of rice farmers in the San Martín region.

show abstract

Section: Discussionmentioning

confidence: 99%

Co-Inoculation of Endophytes Bacillus siamensis TUR07-02b and Priestia megaterium SMBH14-02 Promotes Growth in Rice with Low Doses of Nitrogen Fertilizer

Ríos-Ruiz

Tuanama-Reátegui

Huamán-Córdova

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…To increase the screening rate of the target strains, we selected the rice varieties with resistance to rice blast, and also enriched the endophytic bacteria in the seed samples to avoid failure due to too few endophytic bacteria or too low concentration in the samples. In the past, our research group used traditional methods to screen and explore the endophytic bacteria with antagonistic activity against pathogenic fungi in plant seeds, and the entire experimental process took at least one week or more (Jing et al 2020;Yang et al 2020). By consulting the method description in other related reports, we can also nd that it takes a long time from strain screening to the completion of antagonistic experiments (Liu et al 2016 et al 2020).…”

Section: Resultsmentioning

confidence: 99%

A High-Throughput Screening Method for Endophytic Bacteria With Antagonistic Activity Against Magnaporthe Oryzae in Rice (Oryza Sativa L.) Seeds

Wang

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Screening for microorganisms with antagonistic activity against pathogens in crops is of great significance for the preparation of microbial antagonists, the control of crop diseases, and the sustainable development of agriculture. Through the experiment, a rapid, efficient, and one-step high-throughput method for screening endophytic bacteria with antagonistic activity against Magnaporthe oryzae in rice seeds was successfully established, and the experimental results showed that the endophytic bacteria with antagonistic activity of Magnaporthe oryzae ACCC 36020 could be directly screened from six groups of experimental samples by this method. The establishment of this method can achieve simultaneous screening and purification, one-step high-throughput screening of antagonistic bacteria in rice seeds, and greatly save time.

show abstract

“…Many Bacillus species produce a variety of hydrolytic enzymes, such as cellulase, β-1,3glucanase, and protease, which are responsible for the degradation of diverse components of fungal pathogens [35,37]. The detection of cellulase, protease, and β-1,3-glucanase in BV01 supports its association with the growth suppression of several fungal phytopathogens.…”

Section: Discussionmentioning

confidence: 95%

Bacillus velezensis BV01 Has Broad-Spectrum Biocontrol Potential and the Ability to Promote Plant Growth

Huang,

Zhang,

et al. 2023

Microorganisms

View full text Add to dashboard Cite

To evaluate the potential of a bacterial strain as a fungal disease control agent and plant growth promoter, its inhibitory effects on phytopathogens such as Bipolaris sorokiniana, Botrytis cinerea, Colletotrichum capsici, Fusarium graminearum, F. oxysporum, Neocosmospora rubicola, Rhizoctonia solani, and Verticillium dahliae were investigated. The results showed that the inhibitory rates in dual-culture and sterile filtrate assays against these eight phytopathogens ranged from 57% to 83% and from 36% to 92%. The strain was identified as Bacillus velezensis based on morphological and physiological characterization as well as phylogenetic analyses of 16S rRNA and the gyrase subunit A protein (gyrA) regions. The results demonstrated that B. velezensis was able to produce fungal cell-wall-degrading enzymes, namely, protease, cellulase, and β-1,3-glucanase, and the growth-promotion substances indole-3-acetic acid (IAA) and siderophore. Furthermore, B. velezensis BV01 had significant control effects on wheat root rot and pepper Fusarium wilt in a greenhouse. Potted growth-promotion experiments displayed that BV01 significantly increased the height, stem diameter, and aboveground fresh and dry weights of wheat and pepper. The results imply that B. velezensis BV01, a broad-spectrum biocontrol bacterium, is worth further investigation regarding its practical applications in agriculture.

show abstract

An endophytic strain JK of genus bacillus isolated from the seeds of super hybrid rice (Oryza sativa L., Shenliangyou 5814) has antagonistic activity against rice blast pathogen

Cited by 22 publications

References 41 publications

Co-Inoculation of Endophytes Bacillus siamensis TUR07-02b and Priestia megaterium SMBH14-02 Promotes Growth in Rice with Low Doses of Nitrogen Fertilizer

Co-Inoculation of Endophytes Bacillus siamensis TUR07-02b and Priestia megaterium SMBH14-02 Promotes Growth in Rice with Low Doses of Nitrogen Fertilizer

A High-Throughput Screening Method for Endophytic Bacteria With Antagonistic Activity Against Magnaporthe Oryzae in Rice (Oryza Sativa L.) Seeds

Bacillus velezensis BV01 Has Broad-Spectrum Biocontrol Potential and the Ability to Promote Plant Growth

Contact Info

Product

Resources

About