An endosomal LAPF is required for macrophage endocytosis and elimination of bacteria

Li, Tianliang; Qin, Ken; Li, Nan; Han, Chaofeng; Cao, Xuetao

doi:10.1073/pnas.1903896116

Cited by 24 publications

(16 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, some biomacromolecules and polymers without aromatic rings, including protein fibrils, polypeptides, poly(amido amine)s (PAMAMs), polyethylenimine (PEI), hyperbranched poly(amino ester), and polyurea dendrimers, were reported to possess remarkable fluorescence in the aggregated state and in solution, confirming that aromatic groups are not the basic requirement for photoluminescence. Accordingly, some other mechanisms have been proposed, such as cluster‐triggered emission, aggregation‐induced emission (AIE), crosslink‐enhanced emission (CEE), and so on. Regardless of the different names, these mechanisms are mainly related to the interactions of polymer chains or molecules.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence of Nonaromatic Organic Systems and Room Temperature Phosphorescence of Organic Luminogens: The Intrinsic Principle and Recent Progress

Tang

et al. 2018

Small

215

117

View full text Add to dashboard Cite

Following the development of photoluminescence systems with various compositions, some nontraditional structures, including nonaromatic organic systems as fluorophores and organic luminogens as the source of phosphorescence emission at room temperature, have attracted considerable attention for their advantages in biological and medical applications, and for the updated photophysical understandings in science. In this Review, the recent progress in understanding these organic compounds or polymers for fluorescence and phosphorescence is briefly summarized, with the aim of exploring the intrinsic principle of these novel photoluminescence systems and providing reasonable constructs for molecular design. Finally, some prospects are suggested for further development of this continually expanding area of research, with the coined concept of Molecular Uniting Set Identified Characteristic (MUSIC).

show abstract

Section: Introductionmentioning

confidence: 99%

Fluorescence of Nonaromatic Organic Systems and Room Temperature Phosphorescence of Organic Luminogens: The Intrinsic Principle and Recent Progress

Tang

et al. 2018

Small

215

117

View full text Add to dashboard Cite

show abstract

“…These changes depend on the intermolecular distances, and the orientation of dye molecules and are the result of electrostatic interactions between the transition moments . The planar structural features of common xanthene derivatives facilitate π‐π interaction between the dye molecules, which also interact through various other interactions (e. g. inter or intra molecular charge transfer, energy transfer and excited state reactions), resulting in aggregation caused quenching (ACQ) . Thus, low Rh6G emission at 0 min could be attributed to the fact that Rh6G particles freshly scattered on the PSpBSA liq were in aggregated state (as seen in POM data in Figure e and 2f), resulting in a quenched emission intensity (Figure b).…”

Section: Resultsmentioning

confidence: 99%

Dispersion and Interaction of Charged Fluorescent Dyes in Protein‐Polymer Surfactant‐based Non‐Aqueous Liquid

Mukhopadhyay

Sharma

2020

ChemPhysChem

View full text Add to dashboard Cite

Viscous, non‐aqueous liquid comprising stoichiometric conjugates of polymer surfactant‐bovine serum albumin (PSpBSA) is used as a host matrix for the dispersion of chemically distinct hydrophilic dyes. Using a combination of bright field polarized optical microscopy and fluorescence spectroscopy, we investigate the dispersion of dry and powdered cationic (Rhodamine 6G; Rh6G) and anionic (Fluorescein; FL) dyes in the PSpBSA liquid at room temperature. As the dyes disperse and dissolve in the PSpBSA liquid, it results in a pronounced increase in emission intensity of the former. Interestingly, a shift from 571 to 582 nm is observed in the emission maxima of Rh6G as it disperses in the PSpBSA solvent. Whilst no such red shift is found for the Rh6G dispersion in the aqueous solutions of either native BSA or polymer‐surfactant conjugated BSA, a similar shift occurs when Rh6G is dispersed in neat polymer‐surfactant (PS), suggesting the interaction of the dye with the PS chains. In the case of anionic FL, no shift is observed in its emission maximum as it disperses in the PSpBSA liquid. Furthermore, within 120 minutes of FL dispersion in the PSpBSA liquid, we observe a ≈26 % decrease in the tryptophan emission intensity (λexc.=285 nm; λemi.=330 nm) of BSA, which could be attributed to both static and dynamic quenching. Our findings provide a proof of concept of an alternative non‐aqueous solvent matrix which can dissolve and disperse charged fluorescent dyes, provide suitable binding sites, and show substantial photoluminescence. Thus, it can be envisaged for utilization as an alternative solvent medium for lasing dyes and related applications.

show abstract

“…Our lab previously found that Siglec-G negatively regulated cross-presentation in DCs through SHP1. We recently found that Src-LAPF-Cav1 promoted both bacteria and TLR4 endocytosis (37), which indicated that activating Src might increase anti-bacterial innate immunity. Further studies on Siglec-G in different cells by regulating SHP1/2 and Src activation may reveal the molecular mechanism of innate immune responses.…”

Section: Discussionmentioning

confidence: 99%

Siglec-G Deficiency Ameliorates Hyper-Inflammation and Immune Collapse in Sepsis via Regulating Src Activation

Qin

et al. 2019

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

Hyper-inflammation during acute phase and sequential hypo-inflammation during immunosuppressive phase in macrophages/monocytes lead to multiorgan failure syndrome and immune collapse of sepsis, in which toll-like receptor (TLR)-triggered inflammatory responses play a major role. Here, we reported that Siglecg deficiency attenuated TLR4-triggered pro-inflammatory cytokine production and increased anti-inflammatory cytokine [interleukin-10 [IL-10]] production in vivo and in vitro at both acute and immunosuppressive phases. Siglecg deficiency also protected mice from lipopolysaccharide (LPS)-induced sepsis with less inflammation in the lung and less tissue destruction in the spleen. Siglec-G inhibited proto-oncogene tyrosine-protein kinase Src (Src) activation via recruiting and activating tyrosine phosphatase Src homology region 2 domain-containing phosphatase-1 (SHP1) through immunoreceptor tyrosine-based inhibitory motif (ITIM) domain. Src could inhibit TLR4-induced inflammatory cytokines and promote anti-inflammatory cytokine IL-10. Mechanical investigation showed that Src could interact with and phosphorylate STAT3. Src could also promote HIF1α degradation through activating GSK3β. Our study reveals that Siglec-G orchestrates TLR-induced inflammation, which outlines that blocking Siglec-G or activating Src may be a promising strategy for both acute and chronic inflammatory diseases.

show abstract

An endosomal LAPF is required for macrophage endocytosis and elimination of bacteria

Cited by 24 publications

References 37 publications

Fluorescence of Nonaromatic Organic Systems and Room Temperature Phosphorescence of Organic Luminogens: The Intrinsic Principle and Recent Progress

Fluorescence of Nonaromatic Organic Systems and Room Temperature Phosphorescence of Organic Luminogens: The Intrinsic Principle and Recent Progress

Dispersion and Interaction of Charged Fluorescent Dyes in Protein‐Polymer Surfactant‐based Non‐Aqueous Liquid

Siglec-G Deficiency Ameliorates Hyper-Inflammation and Immune Collapse in Sepsis via Regulating Src Activation

Contact Info

Product

Resources

About