Rotational cone tribometer (RCT) was developed to study the tribological behavior in close to application contact conditions. RCT allows testing of a sample with line contact and also allows direct installation of a radial shaft seal. The RCT test evaluates, on one hand, the frictional behavior of a material sample in line contact, aiding material development for better tribological performance. On the other hand, it is also used to evaluate the frictional behavior of a ready manufactured sealing component. To assess the performance of the tribometer, differently manufactured thermoplastic polyurethane (TPU) and acrylonitrile butadiene rubber (NBR) samples were analyzed in sliding contact against a steel contact partner in a sliding speed range of 1 · 10−3 m · s−1 till 2 m · s−1. The effect of the morphology of TPU on the tribological behavior is studied.