An enhanced apoptosis and a reduced angiogenesis are associated with the inhibition of lung colonisation in animals fed an<i>n</i>-3 polyunsaturated fatty acid-rich diet injected with a highly metastatic murine melanoma line

Mannini, Antonella; Kerstin, Nadja; Calorini, Lido; Mugnai, Gabriele; Ruggieri, Salvatore

doi:10.1017/s0007114508043791

Cited by 16 publications

(18 citation statements)

References 45 publications

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“…Ciu and Ooi demonstrated that NSAIDS and DHA have synergistic effects on the growth of the human melanoma cell line A-375 (30) and, Denkins et al demonstrated that incubation of human melanoma cells with omega-3 FAs downregulated COX-2 expression indicating that these FAs regulate COX-2-mediated invasion in brain metastatic melanoma (31). A few in vivo studies have also been performed, but only with murine melanoma cell lines, and these studies do suggest that dietary omega-3 FAs slow the growth and progression of metastatic murine melanoma (32-34). To the best of our knowledge this is the first study to evaluate the effect of dietary omega-3 FAs on the growth of a human melanoma cell line in vivo .…”

Section: Discussionmentioning

confidence: 99%

Docosahexaenoic acid, G protein–coupled receptors, and melanoma: is G protein–coupled receptor 40 a potential therapeutic target?

Nehra¹,

Pan²,

Le³

et al. 2014

Journal of Surgical Research

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Purpose To determine the effect of docosahexaenoic acid (DHA) on the growth of human melanoma in vitro and in vivo and to better understand the potential role of the G-protein coupled receptors in mediating this effect. Materials and Methods For in vitro studies, human melanoma and control fibroblast cells were treated with DHA and TAK-875 (selective GPR40 agonist) and a cell viability assay was performed to determine cell counts. A murine subcutaneous xenograft model of human melanoma was used to test the effect of dietary treatment with an omega-3 fatty acid (FA) rich diet compared to an omega-6 FA rich diet on the growth of human melanoma in vivo. A similar animal model was used to test the effect of oral TAK-875 on the growth of established melanoma tumors in vivo. Results DHA has an inhibitory effect on the growth of human melanoma both in vitro and in vivo. Tumors from animals on the omega-3 FA rich diet were 69% smaller in weight (P=0.005) and 76% smaller in volume compared to tumors from animals on the omega-6 FA rich diet. TAK-875 has an inhibitory effect on the growth of human melanoma both in vitro and in vivo. Tumors from animals treated with TAK-875 were 46% smaller in weight (P=0.07), 62% smaller in volume (P=0.03) and grew 77% slower (P=0.04) compared to the placebo group. Conclusion DHA and TAK-875 have a profound and selective inhibitory effect on the growth of human melanoma both in vitro and in vivo.

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Section: Discussionmentioning

confidence: 99%

Docosahexaenoic acid, G protein–coupled receptors, and melanoma: is G protein–coupled receptor 40 a potential therapeutic target?

Nehra¹,

Pan²,

Le³

et al. 2014

Journal of Surgical Research

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“…Recently, Mannini et al . observed that the diffusion of a highly metastatic variant (F10‐SR cells) isolated from the B16 melanoma F10 line and injected intravenously was reduced by feeding host animals a diet containing 5% FO (Table ).…”

Section: Potential Antineoplastic Effect Of Lc N‐3 Pufas Against Malimentioning

confidence: 99%

“…79 It should be noted, however, that 0.01 μM DHA is also two to three orders of magnitude lower than that used (1-50 μM) by others to test the effects of DHA as a PPAR agonist in vitro. 81 Recently, Mannini et al 82 observed that the diffusion of a highly metastatic variant (F10-SR cells) isolated from the B16 melanoma F10 line and injected intravenously was reduced by feeding host animals a diet containing 5% FO ( Table 2). The authors related the reduced metastasis formation to enhanced apoptosis and reduced angiogenesis, as demonstrated by lower von Willebrand factor immunoreactivity in lung colonies of F10-SR cells.…”

Section: Preclinical In Vitro and Animal Studiesmentioning

confidence: 99%

Potential of long-chain n-3 polyunsaturated fatty acids in melanoma prevention

et al. 2014

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The possible antineoplastic activity of dietary long-chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs) has been supported by ample preclinical studies that have identified a number of molecular factors and pathways affected by these fatty acids and involved in cell growth, apoptosis, invasion, and angiogenesis. The aim of this critical review is to assess the current state of knowledge on the potential anticancer effects of LC n-3 PUFAs against malignant melanoma, one of the most common cancers among Western populations. The results of preclinical as well as human observational and interventional studies investigating the effects of LC n-3 PUFAs in melanoma were examined. Overall, the analysis of the literature reveals that, even though a large body of information is available, further effort is needed to identify the main molecular targets of LC n-3 PUFAs in melanoma. Moreover, additional well-designed human observational studies are essential to shed further light on the issue. The results of these studies could provide support and specific information for the development of clinical studies, especially those performed in combination with conventional or innovative antineoplastic therapies.

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“…A number of studies have demonstrated that supplementation with n-3 PUFAs had therapeutic benefits in various disorders [ 93 ]. The omega-3 fatty acids can prevent non-melanoma skin cancers [ 94 ] and melanoma by enhancing apoptosis and reducing angiogenesis; in turn, lung colonization was inhibited in melanoma-bearing animals [ 95 ]. In vitro study by addition of n-3 fatty acid eicosapentaenoic acid or PGE 3 , a metabolite of n-3 fatty acid, in B16 melanoma cell culture showed that the cancer cell growth was significantly inhibited along with the over-expression of phosphatase and tensin homologue deleted on the chromosome 10 (PTEN) protein.…”

Section: Alternative Phytomedicine Treatment Of Melanomamentioning

confidence: 99%

Current Research and Development of Chemotherapeutic Agents for Melanoma

Hsan

Chen

Shyur

2010

Cancers

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Cutaneous malignant melanoma is the most lethal form of skin cancer and an increasingly common disease worldwide. It remains one of the most treatment-refractory malignancies. The current treatment options for patients with metastatic melanoma are limited and in most cases non-curative. This review focuses on conventional chemotherapeutic drugs for melanoma treatment, by a single or combinational agent approach, but also summarizes some potential novel phytoagents discovered from dietary vegetables or traditional herbal medicines as alternative options or future medicine for melanoma prevention. We explore the mode of actions of these natural phytoagents against metastatic melanoma.

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An enhanced apoptosis and a reduced angiogenesis are associated with the inhibition of lung colonisation in animals fed ann-3 polyunsaturated fatty acid-rich diet injected with a highly metastatic murine melanoma line

Cited by 16 publications

References 45 publications

Docosahexaenoic acid, G protein–coupled receptors, and melanoma: is G protein–coupled receptor 40 a potential therapeutic target?

Docosahexaenoic acid, G protein–coupled receptors, and melanoma: is G protein–coupled receptor 40 a potential therapeutic target?

Potential of long-chain n-3 polyunsaturated fatty acids in melanoma prevention

Current Research and Development of Chemotherapeutic Agents for Melanoma

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