An enrichment method for capturing mumps virus whole genome sequences directly from clinical specimens

Frost, Jasmine Rae; Schulz, Helene; McLachlan, Elizabeth; Hiebert, Joanne; Severini, Alberto

doi:10.1016/j.jviromet.2021.114176

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…Nucleic acid was extracted from 200 μl of sample, and first- and second-strand cDNA syntheses were performed as described previously ( 18 ). Libraries were prepared using the TruSeq Nano DNA high-throughput library preparation kit (catalog number 20015965; Illumina), with modifications as described previously ( 18 ). The library was sequenced on the MiSeq platform (Illumina) using a MiSeq reagent kit v3 (catalog number MS-102-3003).…”

Section: Announcementmentioning

confidence: 99%

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Complete Genome Sequence of the World Health Organization Mumps Reference Strain, MuVi/Sheffield.GBR/1.05

Frost

Severini

2022

Microbiol Resour Announc

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Section: Announcementmentioning

confidence: 99%

“…Using the Galaxy platform ( 19 ), FastQ files were aligned using Bowtie 2 ( 20 ) with the settings of pair data set collection and a maximum fragment length of 1,000 bases, with a previously described reference sequence ( 18 ). BAM Coverage Statistics was used to determine genomic coverage ( 21 ).…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of the World Health Organization Mumps Reference Strain, MuVi/Sheffield.GBR/1.05

Frost

Severini

2022

Microbiol Resour Announc

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Optimisation of methodology for whole genome sequencing of Measles Virus directly from patient specimens

Schulz

Hiebert

Frost

et al. 2022

Journal of Virological Methods

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Circulation of Mumps Virus Genotype G in Pakistan During 2023 Outbreak

Umair,

Haider,

Jamal

et al. 2023

Future Virol.

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Aim: Despite recurring mumps outbreaks in Pakistan, the virus's genomic diversity remains unavailable. This study explores the mumps genomic diversity in the 2023 Islamabad outbreak. Methods: During January and February 2023, 32 buccal/throat swabs were collected from suspected mumps cases. 15 tested mumps virus (MuV)-positive via RT-PCR. Positive samples underwent partial SH gene sequencing; a subset (n = 10) was whole-genome sequenced using Illumina MiSeq. Results: Phylogenetic analysis identified genotype-G, sharing 95.57–98.73% homology with Japanese/Canadian isolates. Whole-genome-sequencing (n = 2) confirmed genotype-G, 99% similar to Sheffield/Iowa strain. HN region displayed 95.01–95.36% identity with Jeryl Lynn, with genotype G-specific mutations, except T265I. Conclusion: This is the first report of partial and whole-genome sequencing of MuV from Pakistan, highlighting the necessity for genomic surveillance and vaccine integration.

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An enrichment method for capturing mumps virus whole genome sequences directly from clinical specimens

Cited by 4 publications

References 29 publications

Complete Genome Sequence of the World Health Organization Mumps Reference Strain, MuVi/Sheffield.GBR/1.05

Complete Genome Sequence of the World Health Organization Mumps Reference Strain, MuVi/Sheffield.GBR/1.05

Optimisation of methodology for whole genome sequencing of Measles Virus directly from patient specimens

Circulation of Mumps Virus Genotype G in Pakistan During 2023 Outbreak

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