An environmental Sporothrix as a cause of corneal ulcer

Morrison, Annie S.; Lockhart, Shawn R.; Bromley, Jennifer G.; Kim, Joung Y; Burd, Eileen M.

doi:10.1016/j.mmcr.2013.03.002

Cited by 63 publications

(43 citation statements)

References 11 publications

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“…Based on the latter study, Romeo and collaborators studied the molecular phylogeny and epidemiology of S. schenckii complex strains isolated in Italy, and demonstrated that 26 environmental strains co-clustered with S. pallida and two clinical strains grouped with S. schenckii stricto sensu [13]. Nonetheless, a recent report showed that S. pallida caused keratitis in a corneal transplant recipient [14].…”

Section: Introductionmentioning

confidence: 99%

Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex

Oliveira

Santos

Sampaio

et al. 2015

Research in Microbiology

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Accurate species identification of the Sporothrix schenckii complex is essential, since identification based only on phenotypic characteristics is often inconclusive due to phenotypic variability within the species. We used matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for species identification of 70 environmental and clinical isolates of the Sporothrix complex. A reference database was established for MALDI-TOF MS-based species identification according to minor adjustments in the manufacturer's guidelines. The MALDI-TOF MS clearly distinguished strains of Sporothrix brasiliensis, Sporothrix globosa, Sporothrix mexicana, S. schenckii, Sporothrix luriei and Sporothrix pallida, enabling identification of all isolates at the species level, as confirmed by partial calmodulin gene sequence analyses. The present methodology is simple, reliable, rapid and highly suitable for routine identification in clinical mycology laboratories and culture collections, particularly for updating and reclassifying of deposited Sporothrix isolates.

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Section: Introductionmentioning

confidence: 99%

Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex

Oliveira

Santos

Sampaio

et al. 2015

Research in Microbiology

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“…1 Molecular studies have identified Sporothrix brasiliensis, Sporothrix globosa, Sporothrix luriei, Sporothrix mexicana, Sporothrix pallida and Sporothrix schenckii, the most common species globally, as responsible to sporotrichosis in different regions. [1][2][3][4][5][6][7][8][9] The metropolitan region of Rio de Janeiro is hyperendemic for cat-associated sporotrichosis, 10,11 primarily due to S. brasiliensis. 1,5 S. brasiliensis patients have presented with acute, chronic, fixed, lymphangitic, mucosal, disseminated and relapsing sporotrichosis, as well as manifestations of hypersensitivity.…”

Section: Introductionmentioning

confidence: 99%

Increase in virulence ofSporothrix brasiliensisover five years in a patient with chronic disseminated sporotrichosis

et al. 2015

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The metropolitan region of Rio de Janeiro is hyperendemic for cat-associated sporotrichosis. This study aimed to assess the virulence of serial Sporothrix isolates from a 61-year-old male patient with chronic, destructive disseminated sporotrichosis. Five Sporothrix isolates were cultured from skin exudates and bone samples over a 5-year period, and all were molecularly identified as Sporothrix brasiliensis. The final isolate was significantly more virulent in Galleria mellonella larvae compared to earlier isolates. We conclude that S. brasiliensis has the capacity to increase in virulence in vivo. This finding is significant to clinicians caring for individuals with S. brasiliensis disease and it suggests that further studies are needed to identify the mechanisms underlying pathogenicity enhancement during chronic disease.

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“…Sporothrix pallida was also found to be associated with the native subcortical beetle Ctonoxylon sp. 1 (Musvuugwa, 2014) and is known from many other environments including water sediments, soil, the sporophore of a slime-mould (De Meyer et al 2008) and from a corneal ulcer of a human patient (Morrison et al 2013). This fungus is, therefore, not very host and/or vector specific and may easily move from one environment to the next.…”

Section: Selection Of Study Organismsmentioning

confidence: 99%

Future danger posed by fungi in the Ophiostomatales when encountering new hosts

2016

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The Ophiostomatales contain pathogens that threaten forests world-wide. Global trade increases encounters with new hosts, with potential devastating consequences. We assessed the danger posed by the movement of Ophiostomatales between different host trees in South Africa. We tested the pathogenicity of five fungal species from native South African trees, and three from exotic trees, on various native and exotic trees. To evaluate the potential of fungi to move to new hosts, we investigated the strength of their associations with arthropod vectors. Results indicate that many fungal species are pathogens of newly encountered and distantly-related hosts. Encounters of pathogens with new hosts are less likely when host plants are distantly related, and outside the host range of boring beetle vectors, which also 2 reduces the chances of vectoring by phoretic mite associates. However, pathogens associated with numerous mite species and wounds are more likely to encounter new hosts and pose future threats.

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An environmental Sporothrix as a cause of corneal ulcer

Cited by 63 publications

References 11 publications

Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex

Development and optimization of a new MALDI-TOF protocol for identification of the Sporothrix species complex

Increase in virulence ofSporothrix brasiliensisover five years in a patient with chronic disseminated sporotrichosis

Future danger posed by fungi in the Ophiostomatales when encountering new hosts

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