“…This purpose of the present work was to provide a method that is safer for the operator and the environment by complying with the principles of green chemistry (Anastas and Kirchhoff 2002) as well as being fast, simple, and inexpensive. As an alternative methodology that is eco-friendly, the combination of diffuse reflectance spectroscopy with spot tests has been described for the determination of drugs in routine clinical procedures (Gotardo et al 2004;Lima et al 2009: Capiau et al 2016Ehiwe et al 2016), food analysis (Luiz, Pezza, and Pezza 2012;Rossini et al 2016), quality control (Inagaki et al 2016;Rahoui et al 2016), and determination of contaminants (Tubino, Rossi, and Magalhães 1997;Okparanma, Coulon, and Mouazen 2014).…”