An environmentally regulated receptor for diamine oxidase modulates human endothelial cell/fibroblast histamine degradative uptake.

Baenziger, Nancy Lewis; Mack, P; Jong, Y.-J. I.; Dalemar, L. R.; Pérez, Natacha; Lindberg, C.; Wilhelm, B.; Haddock, R C

doi:10.1016/s0021-9258(17)36549-3

Cited by 15 publications

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Vascular amine oxidases are needed for leukocyte extravasation into inflamed joints in vivo

Marttila-Ichihara¹,

Smith²,

Stolen³

et al. 2006

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. Leukocyte traffic from the blood to the joints is crucial in the pathogenesis of arthritis. A bifunctional endothelial cell-surface glycoprotein, AOC3 (amine oxidase, copper-containing 3; also known as vascular adhesion protein 1), has both adhesive and enzymatic properties. We undertook this study to determine the contribution of AOC3 and its oxidase activity to leukocyte trafficking into inflamed joints in vivo.Methods. We used gene-modified animals, molecular modeling, an AOC3 enzyme inhibitor, oxidase assays, and arthritis models (adjuvant-induced arthritis [AIA] in rats and anti-type II collagen antibodyinduced arthritis in mice) to dissect the importance of AOC3 in vivo.Results. The AOC3 inhibitor fitted well with a covalent binding mode into the active site of the AOC3 crystal structure. It selectively blocked the oxidase activity of AOC3 in enzyme assays. Intraperitoneal and oral administration of the AOC3 inhibitor significantly ameliorated rat AIA. In anti-type II collagen antibodyinduced arthritis in mice, the AOC3 inhibitor also improved the outcome of the joint inflammation. The acute semicarbazide-sensitive amine oxidase blockade by the inhibitor had even more pronounced effects than genetic deletion of AOC3. Enzymatic analyses showed that the inhibitor also blocked 2 other structurally very closely related AOCs, but not any of more than 100 other enzymes tested.Conclusion. These are the first data to demonstrate that the enzymatic activity of the atypical endothelial adhesion molecule AOC3, and possibly that of other closely related ecto-oxidases, is crucial for leukocyte exit from the vessels in inflamed joints in vivo.

show abstract

Vascular amine oxidases are needed for leukocyte extravasation into inflamed joints in vivo

Marttila-Ichihara¹,

Smith²,

Stolen³

et al. 2006

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

Histamine and Biogenic Amines

Jarisch¹,

Wantke²,

Raithel

et al. 2014

Histamine Intolerance

View full text Add to dashboard Cite

Paracellular tightness and catabolism restrict histamine permeation in the proximal colon of pigs

Aschenbach

Ahrens

Garz

et al. 2002

Pfl�gers Archiv European Journal of Physiology

View full text Add to dashboard Cite

Luminal histamine concentrations can be very high in the intestine without eliciting clinical histaminosis. The intestinal mechanisms that possibly prevent systemic intoxication by luminal histamine were investigated in the present study. Porcine colonic epithelia were mounted in Ussing chambers. After unilateral application of partially (3)H-labelled histamine, mucosal-to-serosal (MS) and serosal-to-mucosal (SM) flux rates were calculated from the contralateral appearance of histamine and the radioactive histamine label (hist-rad; representing histamine plus catabolites). A discrepancy between histamine and hist-rad fluxes was observed in both flux directions, indicating efficient histamine catabolism at all histamine concentrations tested (5, 50 and 100 microM). Catabolism exceeded 65% at 100 microM histamine and reached approximately 100% at 5 microM histamine. Blockade of histamine N-methyltransferase by amodiaquin (100 microM) abolished catabolism completely, whereas blockade of diamine oxidase by aminoguanidine (100 microM) was less effective. MS fluxes of histamine and hist-rad correlated with mannitol fluxes. Mast cell stimulation with the calcium ionophore A23187 (1 microM) induced a twofold larger histamine appearance on the serosal side than the mucosal application of 100 microM histamine. It is concluded that histamine permeation across the porcine proximal colon is restricted by low permeability and sequential catabolism by histamine N-methyltransferase and diamine oxidase.

show abstract

An environmentally regulated receptor for diamine oxidase modulates human endothelial cell/fibroblast histamine degradative uptake.

Cited by 15 publications

References 58 publications

Vascular amine oxidases are needed for leukocyte extravasation into inflamed joints in vivo

Vascular amine oxidases are needed for leukocyte extravasation into inflamed joints in vivo

Histamine and Biogenic Amines

Paracellular tightness and catabolism restrict histamine permeation in the proximal colon of pigs

Contact Info

Product

Resources

About