An enzymatic method for the microdetermination of ethanol

“…Animals in one group were sampled at 10, 60, and 120 min fol lowing administration of ethanol and animals in the other group were tested at 30 and 90 min postinjec tion. Serum concentrations of ethanol were measured using the enzymatic determination of ethanol de scribed by Bonnichsen and Theorell [4].…”

Section: Methodsmentioning

confidence: 99%

Response to Thermal Stress in the Rat following Acute Administration of Ethanol

Spiers¹,

Threatte²,

Fregly³

1984

Pharmacology

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Several thermoregulatory responses (i.e., colonic temperature, tail-skin temperature, rate of oxygen consumption) were measured in the rat following acute administration of either saline or 3 g ethanol/kg BW i.p. at ambient temperatures (T_a) of 17, 25, and 32 °C. The magnitude of the ethanol-induced, hypothermic response was inversely related to T_a, with the decrease in colonic temperature (T_co) at 120 min postinjection ranging from 0.1 °C at T_a32 °C to 3.2 °C at T_a 17 °C. Depression of rate of oxygen consumption (heat production) was a major factor contributing to ethanol-induced hypothermia, with no observed differences in heat loss as assessed by differences in the responses of tail skin temperature. Thermoregulatory responses were also measured following acute administration of ethanol at T_a 25 °C and immediate exposure to either T_a 32 or 17 °C. The increase in T_co of ethanol-treated rats was delayed compared to controls following exposure to 32 °C. In addition, tail skin temperature and rate of oxygen consumption of treated rats were significantly lower. The delayed rise in T_co is most likely the result of a reduction in rate of oxygen consumption. When administered ethanol at T_a 25 °C and then exposed to T_a 17 °C, the rats exhibited a significant recovery from the metabolic depression that characterized the administration of ethanol during exposure to T_a 17 ° C. This was most likely related to differences in the clearance of ethanol from blood. The results of this study are consistent with the suggestion that the physiologically significant inhibitory effect of acute administration of 3 g ethanol/kg BW on thermoregulatory responses of rats is manifested at the level of heat production rather than heat loss and that the effect is exacerbated by a reduction in T_a.

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“…The purpose of this urine collection was not named to the participants. Analysis of ethyl alcohol in urine was performed with an alcohol dehydrogenase method according to Bonnichsen (2). Finally the subjects were asked by a modified self-administ- …”

Section: Methodsmentioning

confidence: 99%

Indices of Alcohol Intake

Au¹,

Hellsing²

1980

Upsala Journal of Medical Sciences

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Comparison b e tween performed on t h e v a l i d i t y of serum-gamma-glutamyltransferase (S-GT) and serum-alkaline phosphatase (S-ALP) c o n c e n t r a t i o n s f o r e s t a b l i s h i n gt h e i n c r e a s e d a l c o h o l i n t a k e i n a group of middle-aged men i n Uppsala. These men were a l l born i n 1915 and were p a r t i c i p a n t s of a s p e c i a l h e a l t h i n v e s t i g at i o n performed a t t h e i r age of 60 y e a r s . S u b j e c t s who were r e g i s t e r e d a t t h e

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An enzymatic method for the microdetermination of ethanol

Cited by 267 publications

References 11 publications

Causes of Elevated Serum Gamma-glutamyl Transferase in Patients Attending Outpatient Somatic Clinics and District Health Centres

Causes of Elevated Serum Gamma-glutamyl Transferase in Patients Attending Outpatient Somatic Clinics and District Health Centres

Response to Thermal Stress in the Rat following Acute Administration of Ethanol

Indices of Alcohol Intake

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