An enzyme-free electrochemical biosensor for simultaneous detection of two hemophilia A biomarkers: Combining target recycling with quantum dots-encapsulated metal-organic frameworks for signal amplification

Rezaei, Hadi; Motovali-Bashi, Majid; Radfar, Sasan

doi:10.1016/j.aca.2019.09.037

Cited by 45 publications

(16 citation statements)

References 36 publications

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“…These electroactive species can be inorganic molecules as well as organic ones. For example, metals such as gold nanoparticles [41], silver nanoparticles [42], cadmium [43], and plomb [44] were employed as an inorganic RedOx probe. Organic molecules including, thionine (Thi) [45], ferrocene (Fc) [46], and methylene blue (MB) [47] were mostly used.…”

Section: Electrochemical Biosensor Based On Electroactive Labeled Probe Sequencementioning

confidence: 99%

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Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

et al. 2020

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Cancer is the second most fatal disease in the world and an early diagnosis is important for a successful treatment. Thus, it is necessary to develop fast, sensitive, simple, and inexpensive analytical tools for cancer biomarker detection. MicroRNA (miRNA) is an RNA cancer biomarker where the expression level in body fluid is strongly correlated to cancer. Various biosensors involving the detection of miRNA for cancer diagnosis were developed. The present review offers a comprehensive overview of the recent developments in electrochemical biosensor for miRNA cancer marker detection from 2015 to 2020. The review focuses on the approaches to direct miRNA detection based on the electrochemical signal. It includes a RedOx-labeled probe with different designs, RedOx DNA-intercalating agents, various kinds of RedOx catalysts used to produce a signal response, and finally a free RedOx indicator. Furthermore, the advantages and drawbacks of these approaches are highlighted.

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Section: Electrochemical Biosensor Based On Electroactive Labeled Probe Sequencementioning

confidence: 99%

“…This allows the immobilization of a large number of RedOx molecules after a hybridization reaction leading to the high sensitivity of the biosensor (Figure 5). Nanomaterials can be used as carriers of the RedOx molecule [44]. AuNPs are usually used in this case as they allow easy attachment through the thiol link.…”

Section: Redox Molecules Linker To Nanocarriersmentioning

confidence: 99%

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

et al. 2020

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“…Finally, a solution containing HCl was employed to dissolve the encapsulated PbS and CdS QDs to measure the released Pb(II) and Cd(II) ions by DPV. The biosensor response was linear from 1 fM to 1 µM, with a LOD of 0.19 and 0.28 fM for miR-1246 and miR-4521, respectively [ 46 ].…”

Section: Nanobioconjugates Assemblymentioning

confidence: 99%

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

Cajigas

Orozco

2020

Molecules

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Nanobioconjugates are hybrid materials that result from the coalescence of biomolecules and nanomaterials. They have emerged as a strategy to amplify the signal response in the biosensor field with the potential to enhance the sensitivity and detection limits of analytical assays. This critical review collects a myriad of strategies for the development of nanobioconjugates based on the conjugation of proteins, antibodies, carbohydrates, and DNA/RNA with noble metals, quantum dots, carbon- and magnetic-based nanomaterials, polymers, and complexes. It first discusses nanobioconjugates assembly and characterization to focus on the strategies to amplify a biorecognition event in biosensing, including molecular-, enzymatic-, and electroactive complex-based approaches. It provides some examples, current challenges, and future perspectives of nanobioconjugates for the amplification of signals in electrochemical biosensing.

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“…15,17,18 Currently, quantum dots (QDs) not only have very important applications in solar cells and phototransistor components, but also in biological applications including biosensors, 19,20 bioimaging 21 and biomedical research. 22 Cadmium selenide (CdSe) QDs with a size of 1-20 nm are currently used for in vivo imaging and diagnostic treatment. 23 Growing an outer shell of AP on the outside of the QDs can greatly improve its stability.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism

Song

Xin

et al. 2021

IJN

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Background With the development of bacterial resistance, the range of effective antibiotics is increasingly becoming more limited. The effective use of nanoscale antimicrobial peptides (AP) in therapeutic and diagnostic methods is a strategy for new antibiotics. Methods Combining both AP and cadmium selenide (CdSe) into a composite material may result in a reagent with novel properties, such as enhanced antibacterial activity, fluorescence and favorable stability in aqueous solution. Results AP-loaded CdSe NPs (AP-CdSe NPs) showed strong antibacterial activity against multidrug-resistant (MDR) Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) in vitro and in vivo. Colony-forming unit (CFU) and minimum inhibitory concentration (MIC) assays showed that AP-CdSe NPs have highly effective antibacterial activity. The quantitative analysis of apoptosis by flow cytometry analysis further confirmed that MDR E. coli and S. aureus treated with AP-CdSe NPs had death rates of 98.76% and 99.13%, respectively. Also, AP-CdSe NPs was found to inhibit bacterial activity in an in vivo bacteremia model in mice infected with S. aureus . In addition, the antibacterial mechanism of AP-CdSe NPs was determined by RNA sequencing analysis. Gene ontology (GO) analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis revealed the molecular mechanism of the antibacterial effect of AP-CdSe NPs. Importantly, histopathology analysis, and hematological toxicity analysis indicated that AP-CdSe NPs had few side effects. Conclusion These results demonstrate that AP loaded on CdSe NPs had a higher water solubility, bioavailability and antibacterial effect compared with raw AP. This study reports findings that are helpful for the design and development of antibacterial treatment strategies based on AP.

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An enzyme-free electrochemical biosensor for simultaneous detection of two hemophilia A biomarkers: Combining target recycling with quantum dots-encapsulated metal-organic frameworks for signal amplification

Cited by 45 publications

References 36 publications

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

The Effects of Luminescent CdSe Quantum Dot-Functionalized Antimicrobial Peptides Nanoparticles on Antibacterial Activity and Molecular Mechanism

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