An enzyme immunoassay of phaseolinone and its application in estimation of the amount of toxin in Macrophomina phaseolina-infected seeds

Bhattacharya, Dipanwita; Dhar, Tarun K.; Ali, Esahak

doi:10.1128/aem.58.6.1970-1974.1992

Cited by 12 publications

(7 citation statements)

References 10 publications

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“…Figure 2 shows the amount of absorbed phaseolinone estimated in the seeds at 50% inhibition was 1.2 pg g-' of wet tissue. This value is double the amount of toxin estimated (0.6 pg g-') during infection under sterile conditions (Bhattacharya et al 1992), but in both cases there is a close correlation between the toxin level estimated in the tissues and the pathogenic symptom. The lower tissue toxin level observed at 50% inhibition during infection may be ascribed to the production of other factors (toxins and could now effectively infect the seeds in soil and showed inoculum size-dependent inhibition of seed germination.…”

Section: Resultsmentioning

confidence: 72%

“…For infection of seeds under sterile conditions, the fungus was grown in sterilized wheat bran (2 g) in culture tubes. After 4 d at 30"C, the inoculum was used to infect seeds as described by Bhattacharya et al (1992).…”

Section: Lnfectlon Of Seedsmentioning

confidence: 99%

“…l), the major exotoxin isolated from the culture filtrate of the fungus, was found to be a potent inhibitor of seed germination (Dhar et al 1982;Bhattacharya et al 1987). A previous communication (Bhattacharya et al 1992) described the development of a sensitive enzyme immunoassay (ELISA) method for estimation of the toxin in infected plant tissues. With this ELISA technique, and also with HPLC, it was shown that there was a correlation between the degree of germination inhibition of Phaseolus mungo seeds infected with a virulent strain of the fungus (MPK'83) and the amount of toxin produced in the infected seeds.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of seed germination by Macrophomina phaseolina is related to phaseolinone production

Bhattacharya

Dhar

Siddiqui

et al. 1994

Journal of Applied Bacteriology

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The production of phaseolinone, a phytotoxic metabolite of Macrophomina phaseolina in infected Phaseolus mungo seeds grown on soil, was estimated by enzyme‐linked immunosorbent assay and HPLC. The degree of inhibition of seed germination correlated well with the amount of toxin produced; 50% inhibition was observed at a toxin level of 2.1 μg g‐1 of wet tissue. A comparison of the toxin‐producing ability of nine isolates of the fungus obtained from different hosts and localities showed that the strain MPK'83 produced a significantly larger amount of the toxin, both in liquid culture and in infected seeds. The virulence of the isolates was related to their ability to produce phaseolinone.

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Section: Resultsmentioning

confidence: 72%

Section: Lnfectlon Of Seedsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibition of seed germination by Macrophomina phaseolina is related to phaseolinone production

Bhattacharya

Dhar

Siddiqui

et al. 1994

Journal of Applied Bacteriology

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“…6c). Phaseolinone activities were routinely detected in all injured tissues using a microtiter plate-based enzyme immunoassay (Bhattacharya et al, 1992). Guayule genotypes responded differentially to phaseohnone and these responses were highly correlated (r = 0.95, P = 0.0]) 308 KuTi et al 'Disease and phytotoxicity severity on the seedlings were rated on a scale of 1-5 by subjective observations; 1 = no visible phytotoKicity/lowest incidence of charcoal rot lesions and 5 = severe phytotoxicity or plant death/highest incidence of charcoal rot lesions.…”

Section: Resultsmentioning

confidence: 99%

“…The toxin (phaseolinone) was obtained from 14-day-old cultures of M. phaseolina in Czapek-Dox broth liquid medium and partially purified as described by Dhingra and Sinclair (1974) and Siddiqui et al (1979). For routine determination of phaseolinone activity, a microtiter plate-based enzyme immunoassay developed by Bhattacharya et al (1992) was used.…”

Section: Toxin Productionmentioning

confidence: 99%

Differential Responses of Guayule (Parthenium argentatum Gray) Genotypes to Culture Filtrate and Toxin from Macrophomina phaseolina (Tassi) Goidanich

Kuti

Schading²,

Latigo

et al. 1997

Journal of Phytopathology

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The potential for using cell-free culture filtrate (CFCF) and toxin (phaseolinone) from Macrophomina phaseolina for rapid and efTective screening procedures for charcoal rot resistance in guayule (Parthenium argentatum) germplasm was assessed. The CFCF and partially purified phaseolinone were incorporated into modified Murashige and Skoog solid medium at the rates of 0-100% (v/v) and 0-1000^g ml"', respectively. The medium pH was adjusted to 5.8 before solidifying with 0.8% agar. Fourweek-old seedlings of 10 guayule genotypes were planted in the medium, incubated and rated for phytotoxic symptoms and tissue damage over a 15-day period. In a greenhouse study, seedling growth, phytotoxicity and damage severity were compared in 12-week-o!d guayule seedlings root-inoculated with M. phaseolina microsderotia. There were significant differences (P = 0.05) in genotypic responses to the fungus, the filtrate and the toxin inoculations. Time until phytotoxic symptoms developed was inversely related to the concentrations of CFCF and the toxin. Phytotoxic symptoms were produced 6 days after exposure to 50% CFCF and 48 h after exposure to 1000/jg ml"' of partially purified phaseolinone. A comparison of photomicrographs of the control and toxintreated root tissues revealed no damage to the control roots and extensive damage to epidermal layers of the treated roots, which was evident 48 h after exposure to lOO^g ml"' level of phaseolinone. Significant correlations were found between tolerance to the fungus and insensitivity to the culture filtrate (r = 0.89, P = 0.05) and the toxin (r = 0.95, P = 0.001) suggesting the possibility of screening for resistance to M. phaseolina using CFCF or phaseolinone. The genotypic reactions to the CFCF were also correlated with reactions to the toxin {r = 0.90, P = 0.05). Guayule breeding lines 'UCIOT and 'P3-r exhibited the greatest tolerance to the pathogen and insensitivity to the CFCF or the toxin whereas 'Cal6', 'Cal7', 'N576', 'N9-5', M1605' and 'N6-5' were very susceptible to the pathogen and sensitive to the CFCF or the toxin. Zusammenfassung Unterscbiedliche Reaktionen von Guayole-Genotypen {Par-themum argentatum Gray) gegenuber dem Kulturflltrat and dem Toxin von Macrophomina pkaseolina (Tassi) GoidanichUntersucht wurde die mogliche Anwendung von zellfreiem Kulturfiltrat (CFCF) und dem Toxin (Phaseolinon) von Macrophomina phaseolina in einem schnellen und zuverlassigen Verfahren bei der Resistenzprtlfung in Guayule {Parthenium argentatum). Das CFCF und das teilgereinigte Phaseolinon wurden dem modifizierten Murashige und Skoog-Agarmedium in den Konzentrationen 0 bis 100% (v/v) bzw. 0 bis 1000 /ig/ml beigemischt. Der pH-Wert des Mediums wurde bei 5,8 eingestellt und anschlieBend mit 0,8% Agar versehen. Vier Wochen alte Samlinge von 10 Guayule-Genotypen wurden in diesen Medien gepflanzt, inkubiert und bonitiert auf phytotoxische Symptome und Gewebezerstorung uber einem Zeitraum von 15 Tagen. Das Samlingswachstum, die Phytotoxizitat sowie die Befallsintensitat wurden in Gewachshausversuc...

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Effect of Temperature and Defense Response on the Severity of Dry Root Rot Disease in Chickpea Caused by Macrophomina phaseolina

Preeti

Panwar

Saini³

et al. 2023

Rhizosphere Biology

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An enzyme immunoassay of phaseolinone and its application in estimation of the amount of toxin in Macrophomina phaseolina-infected seeds

Cited by 12 publications

References 10 publications

Inhibition of seed germination by Macrophomina phaseolina is related to phaseolinone production

Inhibition of seed germination by Macrophomina phaseolina is related to phaseolinone production

Differential Responses of Guayule (Parthenium argentatum Gray) Genotypes to Culture Filtrate and Toxin from Macrophomina phaseolina (Tassi) Goidanich

Effect of Temperature and Defense Response on the Severity of Dry Root Rot Disease in Chickpea Caused by Macrophomina phaseolina

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