An Enzyme-Induced Novel Biosensor for the Sensitive Electrochemical Determination of Isoniazid

Abstract

Abstract:In this present work, a glassy carbon electrode (GCE) was modified primarily with multiwalled carbon nanotubes (MWCNTs) and a composite of MWCNTs and titanium oxide nanoparticles (TiO 2 NPs). The enzyme horseradish peroxidase (HRP) was immobilized to enhance the sensing ability of GCE. The proposed biosensor was used for the sensitive determination of isoniazid (INZ) in various pharmaceutical samples. The electrochemical behaviour of the developed MWCNT-TiO 2 NPs-HRP-GCE biosensor was studied by using cyclic voltammetry (CV) and differential pulse voltammetric (DPV) techniques. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), thermogravimetry (TGA) and transmission electron microscopy (TEM) techniques were used to characterize the developed sensor. Phosphate buffer solution (PBS) with pH 7 was used as supporting electrolyte in the present investigation. The cyclic voltammetric results revealed that the increment of anodic peak currents for the enzyme-induced sensor was almost 8-fold greater than that of a bare GCE. The DPV technique exhibited good limit of detection and limit of quantification values, viz., 0.0335 µM and 0.1118 µM, respectively. Moreover, the developed sensor showed long-lasting stability and repeatability without any interferents. This strongly indicates that the fabricated sensor shows outstanding electrochemical performance towards INZ, with excellent selectivity and sensitivity. The developed sensor was successfully applied to pharmaceutical samples and gave good percentages of recoveries.

Bimetallic cobalt–iron diselenide nanorod modified glassy carbon electrode: an electrochemical sensing platform for the selective detection of isoniazid

“…8B shows the schematic presentation for electrochemical oxidation mechanism of INZ over modified FeCoSe 2 /GCE. 39 These outcomes verified that the projected FeCoSe 2 /GCE is efficiently accomplished for the voltammetric sensing of INZ.…”

Carbon Nanomaterials (CNMs) and Enzymes: From Nanozymes to CNM-Enzyme Conjugates and Biodegradation

“…The most popular target molecule is glucose for biometric health monitoring ( Table 2 ) [ 224 , 227 , 228 , 237 , 249 , 269 , 271 , 273 ]. However, biosensors have been developed to detect many other bioactive compounds too, such as cholesterol [ 312 , 370 , 371 ] and triglycerides [ 282 ], lactose [ 220 ] and lactate [ 225 , 280 ], neurotransmitters [ 234 , 240 , 246 , 278 , 279 , 372 ] and hormones [ 239 ], various disease biomarkers [ 244 , 257 , 261 ], microRNAs [ 223 ], drugs [ 218 , 287 ], pathogens [ 219 ] and toxins [ 221 ], xanthine [ 262 ] and caffeic acid [ 373 ], p -coumaric acid [ 232 ], ferulic acid [ 233 ], trace metals [ 274 ], and oxygen [ 268 ].…”

Behaviour of the faujasite zeolite occluded with Ti(IV) and Zr(IV) and its interaction with Nickel Hexacyanoferrate (III) : A comparative study in the voltammetric detection of isoniazid

“…When associated with rifampicin, IZN is widely employed in the prevention and treatment of tuberculosis, displaying bacteriostatic and bactericidal action and interfering with the metabolism of nucleic acids, bacterial proteins, carbohydrates, and lipids [14,15]. However, high IZN concentrations can lead to intoxication, infammation, loss of liver function, epilepsy and even death when administered in long-term therapies.…”