An enzyme linked immunosorbent assay to diagnose Babesia bovis infection in cattle

Abstract

The optimum gel filtration fraction from lysate of Babesia bovis infected erythrocytes was determined for use as an antigen in an ELISA to diagnose B. bovis infection in cattle. Of four enzyme labels tested, horseradish peroxidase was the most suitable. The assay is both sensitive and specific in detecting antibody for 2-4 years after a single infection. False positive reactions were obtained only with some sera from some Anaplasma marginale infected cattle.

On the High Seroprevalence of Bovine Babesiosis in Wynad District of Kerala

“…Alternatively, sera in dilution 1:120 or greater may preferably be used for interpretation of IFAT results. where it is possible to isolate babesial protein antigens relatively free from host erythrocyte contaminants (Waltisbuhl et al, 1987;Bose et al, 1990;1995;Bose and Peymann, 1994). To overcome the limitations of ELISAs with crude antigens for B. bigemina serological diagnosis, the use of recombinant antigens is generally recommended (OIE Manual, 2000).…”

Avaliação de um ELISA para detecção de anticorpos contra Babesia bigemina em bovinos e sua aplicação em um inquérito sorológico no Brasil

“…Among the current methods of diagnosis, ELISA test has been increasingly used. Nevertheless, one of the problems in developing ELISAs for babesial infections is the contamination of antigen preparations with host proteins (Waltisbuhl et al 1987, Gray & Kaye 1991, el-Ghaysh et al 1996.…”

“…Problems in using ELISA with crude antigen for detection of antibodies to B. bigemina have been reported, mainly concerning it's lack of specificity (Waltisbuhl et al 1987, Gray & Kaye, 1991, el-Ghaysh et al 1996. Such antigens frequently contain host cell components, which may affect the performance of the test by increasing the non-specific background (Passos et al 1998).…”

Karadeni̇z Bölgesi̇ Sigirlarinda Bulunan Kan Parazi̇tleri̇ni̇n Sero-İnsi̇densi̇ Üzeri̇ne Araştirmalar

“…Immunf/uoreszenz Test erwiesen sich 47 Tieren als positiv für B. bigemina, 34 (16,19,22,38,40). Fakat bu testlerden lFA Tın diğerlerine göre basit, kesin ve çabuk sonuç vermesi bakımından, son yıllarda en çok kullanılan test olarak seçildiği bildirilmektedir (7,14,25,38,40,41).…”

“…Fakat bu testlerden lFA Tın diğerlerine göre basit, kesin ve çabuk sonuç vermesi bakımından, son yıllarda en çok kullanılan test olarak seçildiği bildirilmektedir (7,14,25,38,40,41).…”