An enzyme-linked immunosorbent assay for detection of pyrene and related polycyclic aromatic hydrocarbons

Polyelectrolyte nanocapsule probe for the determination of imidacloprid in agricultural food samples

“…The reaction was terminated by stop solution (50 µL) and the OD values were measured with Epoch Microplate Spectrophotometer at 450 nm. The IC 50 value (50% inhibition) for each compound was measured by the inhibition formula (Meng et al, 2015): Inhibition (%) = [1 − B/B 0 ] × 100. B was the corresponding OD 450 values at different concentrations of imidacloprid, B 0 was the OD 450 value without imidacloprid.…”

“…Subsequently, all samples were extracted by ultrasonic extraction in 5% (v/v) methanol in PBS for 10 min and centrifuged for 10 min at 4000 × g. The supernatant was collected and the solutions were comparatively analyzed by the developed assay and commercial kit. The recoveries were calculated according to the following equation (Meng et al, 2015):…”

Development of an indirect competitive ELISA for the detection of acenaphthene and pyrene

“…All animal experiments were conducted in accordance with the guide for the care and use of laboratory animals (Gaber et al, 2010). Eight-week-old female BALB/c mice were immunized with ALA-BSA via hind footpad (Meng et al, 2015). Briefly, 100 µg of immunizing antigen (in 25 μL PBS) with 25 μL of FCA was emulsified and injected hind footpad for each mouse in the first immunization.…”

“…However, these techniques require elaborate sample preparation and skilled analysts. Few immunoassays have also been reported, such as fluorescence polarization immunoassay for benzo [a]pyrene, naphthalene and anthracene (Spier et al, 2009), competitive ELISA for pyrene, chrysene, benzo[a]pyrene, benzo[a]anthracene and indeno [1,2,3-cd]pyrene (Meng et al, 2015), intramolecular energy transfer probe (Kupstat, Knopp, Niessner, & Kumke, 2010), multichannel surface plasmon resonance (SPR) biosensor (Dostálek, Přibyl, Homola, & Skládal, 2007), photoelectrochemical immunosensor (Kang et al, 2011), electrochemical immunosensor and surface infrared immunosensors for benzo[a]pyrene (Ahmad & Moore, 2012;Boujday, Nasri, Salmain, & Pradier, 2010).…”

“…Monoclonal antibody (mAb) based enzyme-linked immunosorbent assay (ELISA) has been widely applied in pollutant detection in environment Meng et al, 2015;Spier et al, 2009). Compared with instrumental methods, ELISA does not require sophisticated instrumentation, skilled analysts and labor intensive sample preparation.…”