An enzyme‐linked immunosorbent assay for the quantitation of IgG anti‐D and IgG subclasses in the sera of alloimmunized patients

Abstract

The method described is sensitive and can be used routinely for the quantitative determination of specific IgG anti-D and IgG subclasses in sera.

Quantification of anti‐D and fetomaternal hemorrhageby flow cytometry

“…It is anticipated that prophylactic anti‐D may therefore be accurately quantified by both methods using these standards, although no comparison has yet been performed. Recently, excellent correlations between IgG enzyme‐linked immunosorbent assay (ELISA) of sensitized, washed, solubilized RBCs 11 and AutoAnalyzer quantification were obtained for the anti‐D standard (IRP 68/419), 10,12 prophylactic anti‐D immunoglobulin preparations, 10,12 and individual sera from D‐immunized donors, 10,12 but the correlation was slightly less for testing of antenatal sera, with one‐third of the results being greater by AutoAnalyzer than by ELISA 13 . A comparison of these ELISAs with FC would be informative; both involve washing the sensitized cells before detection of the anti‐D with anti‐IgG reagents.…”

Measurement of the affinity of anti‐D in the serum of immunized mothers and in immunoglobulin preparations with unlabeled antibodies

“…Dilution of samples in PBS‐BSA was carried out with an automatic diluter (Microlab Hamilton, Touzard‐Matignon, Villeneuve la Garenne, France). The polyclonal anti‐D concentration in each serum sample or immunoglobulin preparation was first determined by ELISA 20 . Serum samples and immunoglobulin were then diluted to six concentrations ranging approximately from 10 to 0.3 µg per mL of anti‐D.…”

Establishment of human heterohybridoma and lymphoblastoid cell lines specific for the Rh D and C antigens

“…The antigen (Rh)‐specific ELISA was performed as basically reported by Lambin and colleagues (Lambin et al ., 1998; Ahaded et al ., 1999). Briefly, a 10% suspension of RBC (group O R 1 R 2 ) was prepared in LISS buffer.…”