An Enzyme-linked Immunosorbent Assay for Genistein 7-O
-[α
-rhamnopyranosyl-(1→6)]-β
-glucopyranoside Determination in Derris scandens
 using a Polyclonal Antibody

Jutathis, Kamonthip; Kitisripanya, Tharita; Udomsin, Orapin; Inyai, Chadathorn; Sritularak, Boonchoo; Tanaka, Hiroyuki; Putalun, Waraporn

doi:10.1002/pca.2633

Cited by 6 publications

(27 citation statements)

References 14 publications

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“…This fingerprint pattern can be employed as a tool for the simple identification of DS stem samples since it reflects the presence and relative abundance of the main compounds. Previous studies [4,9] did not primarily focus on the validation of the analytical method for prenylated isoflavones. In the present study, we developed and validated an HPLC method enabling the simultaneous determination of the glycoside form (GTG), aglycone form (genistein), and the key prenylated derivatives of isoflavones.…”

Section: Resultsmentioning

confidence: 99%

“…Genistein, GTG, and three prenylated isoflavones (derrisisoflavone A, lupalbigenin, and 6,8-diprenylgenistein) were analyzed using high-performance liquid chromatography (HPLC) with a gradient mobile phase system. The HPLC system was based on the method developed in a previous study [9]. The analysis was performed on a Shimadzu high-performance liquid chromatography (HPLC) system.…”

Section: Hplc System For Determination Of Isoflavones In Ds Extractsmentioning

confidence: 99%

“…Previous studies [4,9] did not primarily focus on the validation of the analytical method for prenylated isoflavones. In the present study, we developed and validated an HPLC method enabling the simultaneous determination of the glycoside form (GTG), aglycone form (genistein), and the key prenylated derivatives of isoflavones.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…The isoflavones analyzed included GTG, genistein, lupalbigenin, and scandenin [4]. Additionally, a previous study [9] conducted an HPLC-UV analysis specifically for the determination of GTG. The 95 % ethanol extract of the DS stem consisted predominantly of derrisisoflavone A and lupalbigenin [10].…”

Section: Introductionmentioning

confidence: 99%

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Identification of Major Bioactive Anti-inflammatory Compounds of Derris scandens Stem Using RAW 264.7 Cells and HPLC-UV Analysis

Sae-Foo,

Yusakul,

Nualkaew

et al. 2023

Planta Med

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Derris scandens (DS), is widely recognized for its therapeutic properties, specifically its analgesic effects, which significantly alleviate muscle pain. The chemical constituents of DS stem include various isoflavone derivatives. However, there is currently a lack of specified anti-inflammatory chemical markers and analytical methods for quality control. The present study aimed to evaluate the anti-inflammatory activity of DS and its constituents using the RAW 264.7 cell model. The expression of inflammatory genes such as inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), and 5-lipoxygenase (5-LOX) was examined using quantitative RT-PCR. An high-performance liquid chromatography with UV detection method was developed to quantitatively analyze genistein-7-O-[α-rhamnopyranosyl-(1→6)]-β-glucopyranoside, genistein, derrisisoflavone A, lupalbigenin, and 6,8-diprenylgenistein in DS stem. The developed HPLC-UV method demonstrated high sensitivity with limits of detection and quantification ranging from 0.01 to 0.06 µg/mL and 0.03 to 0.18 µg/mL, respectively. The accuracy of the method ranged from 93.3 to 109.6%. Furthermore, the repeatability and reproducibility of the method were suitable, as indicated by the relative standard deviations of ≤3.02% and ≤6.22%, respectively. The DS extract notably inhibited NO production, exhibiting effects comparable to those of 500 µM diclofenac, and substantially suppressed the expression of iNOS, COX-2, IL-6, and 5-LOX of lipopolysaccharide (LPS) induced genes. For isoflavone derivatives, the order of inhibiting NO production is genistein > lupalbigenin > derrisisoflavone A > 6,8-diprenylgenistein > genistein-7-O-[α-rhamnopyranosyl-(1→6)]-β-glucopyranoside. Genistein, derrisisoflavone A and 6,8-diprenylgenistein significantly suppressed the upregulation of all LPS-induced genes. Consequently, these compounds are recommended as anti-inflammatory markers for quantitative chemical analysis of DS.

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Section: Resultsmentioning

confidence: 99%

Section: Hplc System For Determination Of Isoflavones In Ds Extractsmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of Major Bioactive Anti-inflammatory Compounds of Derris scandens Stem Using RAW 264.7 Cells and HPLC-UV Analysis

Sae-Foo,

Yusakul,

Nualkaew

et al. 2023

Planta Med

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“…Derris scandens (Hog Creeper Vine), commonly known as “Thao Wan Priang” in Thai, is a woody climbing vine in the Fabaceae family. This herb possesses analgesic and anti-inflammatory effects 1 . Isoflavonoids and their prenylated derivatives are the major compounds found in D. scandens.…”

mentioning

confidence: 99%

Estrogenic activity of isoflavones derived from Derris scandens using MCF-7 cell

et al. 2022

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Development and characterisation of highly specific monoclonal antibody‐based immunoassays for the detection and quantification of genistein‐7‐O‐[α‐rhamnopyranosyl‐(1→6)]‐β‐glucopyranoside in Derris scandens (Roxb.) Benth.

Sae‐Foo,

Singkham,

Srisongkhram

et al. 2023

Phytochemical Analysis

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IntroductionThe stem of the plant species Derris scandens (Roxb.) Benth. (DS) contains genistein‐7‐O‐[α‐rhamnopyranosyl‐(1→6)]‐β‐glucopyranoside (GTG), which is a unique marker. Previous analyses of GTG using antibody‐based immunoassays were compromised because of their high cross‐reactivity with structurally related compounds of DS, thereby limiting their applicability in DS quality control.ObjectiveConjugation of GTG with carrier proteins was achieved using the Mannich reaction to produce a highly specific monoclonal antibody (mAb) targeting GTG (anti‐GTG mAb).MethodsThe anti‐GTG mAb was generated using hybridoma technology and characterised using an indirect competitive enzyme‐linked immunosorbent assay (icELISA). Both lateral‐flow immunoassay (LFIA) and icELISA were developed to detect and quantify GTG in DS raw materials and associated products.ResultsicELISA using the anti‐GTG mAb showed 100% specificity for GTG, with only 1.77% cross‐reactivity with genistin and less than 0.01% cross‐reactivity with other compounds. icELISA demonstrated a linear range for GTG determination between 62.5 and 2000 ng/mL. The limits of detection (LOD) and quantification were 49.68 and 62.50 ng/mL for GTG, respectively. The precision of the analysis ranged from 1.28% to 4.20% for repeatability and from 1.03% to 7.05% for reproducibility. The accuracy of the analysis ranged from 101.97% to 104.01% for GTG recovery. GTG levels determined via icELISA were consistent with those confirmed via high‐performance liquid chromatography (HPLC) (R2 = 0.9903). Moreover, the LOD of LFIA for GTG was 500 ng/mL.ConclusionImmunoassays utilising specific anti‐GTG mAbs were successfully developed, including LFIA for rapid GTG detection and icELISA for GTG quantification.

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An Enzyme-linked Immunosorbent Assay for Genistein 7-O -[α -rhamnopyranosyl-(1→6)]-β -glucopyranoside Determination in Derris scandens using a Polyclonal Antibody

Cited by 6 publications

References 14 publications

Identification of Major Bioactive Anti-inflammatory Compounds of Derris scandens Stem Using RAW 264.7 Cells and HPLC-UV Analysis

Identification of Major Bioactive Anti-inflammatory Compounds of Derris scandens Stem Using RAW 264.7 Cells and HPLC-UV Analysis

Estrogenic activity of isoflavones derived from Derris scandens using MCF-7 cell

Development and characterisation of highly specific monoclonal antibody‐based immunoassays for the detection and quantification of genistein‐7‐O‐[α‐rhamnopyranosyl‐(1→6)]‐β‐glucopyranoside in Derris scandens (Roxb.) Benth.

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