An enzyme similar to animal type II photolyases mediates photoreactivation in Arabidopsis.

Ahmad, Margaret; Jarillo, José A.; Klimczak, Leszek J.; Landry, Laurie G.; Peng, Tao; Cashmore, Anthony R.

doi:10.1105/tpc.9.2.199

Cited by 121 publications

(60 citation statements)

References 37 publications

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“…For example, the induction by far-red light in etiolated seedlings of Phaseolus vulgaris and Sinapis alba, by blue light in etiolated seedlings of Arabidopsis thaliana and P. vulgaris, and by UVA in etiolated A. thaliana seedlings has been documented (Langer and Wellmann 1990;Buchholz et al 1995;Ahmad et al 1997). Surprisingly, photolyase expression was not induced by UVB irradiation in etiolated Arabidopsis seedlings (Ahmad et al 1997). On the other hand, light-grown Arabidopsis seedlings exhibit higher CPD photolyase expression levels and activities when treated with UVB (Pang and Hays 1991;Ries et al 2000;Waterworth et al 2002).…”

Section: Introductionmentioning

confidence: 93%

An unidentified ultraviolet-B-specific photoreceptor mediates transcriptional activation of the cyclobutane pyrimidine dimer photolyase gene in plants

Ioki

Takahashi

Nakajima

et al. 2008

Planta

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Cyclobutane pyrimidine dimers (CPDs) constitute a majority of DNA lesions caused by ultraviolet-B (UVB). CPD photolyase, which rapidly repairs CPDs, is essential for plant survival under sunlight containing UVB. Our earlier results that the transcription of the cucumber CPD photolyase gene (CsPHR) was activated by light have prompted us to propose that this light-driven transcriptional activation would allow plants to meet the need of the photolyase activity upon challenges of UVB from sunlight. However, molecular mechanisms underlying the light-dependent transcriptional activation of CsPHR were unknown. In order to understand spectroscopic aspects of the plant response, we investigated the wavelength-dependence (action spectra) of the light-dependent transcriptional activation of CsPHR. In both cucumber seedlings and transgenic Arabidopsis seedlings expressing reporter genes under the control of the CsPHR promoter, the action spectra exhibited the most predominant peak in the long-wavelength UVB waveband (around 310 nm). In addition, a 95-bp cis-acting region in the CsPHR promoter was identified to be essential for the UVB-driven transcriptional activation of CsPHR. Thus, we concluded that the photoperception of long-wavelength UVB by UVB photoreceptor(s) led to the induction of the CsPHR transcription via a conserved cis-acting element.

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Section: Introductionmentioning

confidence: 93%

An unidentified ultraviolet-B-specific photoreceptor mediates transcriptional activation of the cyclobutane pyrimidine dimer photolyase gene in plants

Ioki

Takahashi

Nakajima

et al. 2008

Planta

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“…Not only can a loss of productivity be expected (Borneman and Teramura 1993;Jansen et al 1998;Ballaré et al 1999;Kumagai et al 2001) but also an increase of recombination in the genome, which has been described by Ries et al (2000). In plants, UV-B photoproducts can be removed from DNA by nucleotide excision repair (Gallego et al 2000;Liu et al 2000Liu et al , 2001 or by photoreactivation (Ahmad et al 1997;Landry et al 1997;Nakajima et al 1998). During photoreactivation, the DNA photolyases catalyse the repair of damaged DNA using the energy from photons in the blue/UV-A region (Sancar 2003).…”

Section: Introductionmentioning

confidence: 94%

Increased DNA repair in Arabidopsis plants overexpressing CPD photolyase

Kaiser

Kleiner

Beißwenger

et al. 2009

Planta

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Ultraviolet-B (UV-B, 280-320 nm) radiation may have severe negative effects on plants including damage to their genetic information. UV protection and DNA-repair mechanisms have evolved to either avoid or repair such damage. Since autotrophic plants are dependent on sunlight for their energy supply, an increase in the amount of UV-B reaching the earth's surface may affect the integrity of their genetic information if DNA damage is not repaired efficiently and rapidly. Here we show that overexpression of cyclobutane pyrimidine dimer (CPD) photolyase (EC 4.1.99.3) in Arabidopsis thaliana (L.), which catalyses the reversion of the major UV-B photoproduct in DNA (CPDs), strongly enhances the repair of CPDs and results in a moderate increase of biomass production under elevated UV-B.

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“…In Arabidopsis, the genes responsible for the DNA repair mechanism have been cloned by analyzing UVhypersensitive mutants (Ahmad et al 1997;Nakajima et al 1998;Liu et al 2000Liu et al , 2001. Comparison between the chromosomal location of the rice homologues of these genes and that of qUVR-10 is an alternative approach to the identification of qUVR-10.…”

Section: Discussionmentioning

confidence: 99%

“…Several mutants that are hypersensitive to UV radiation have been isolated (Harlow et al 1994;Jenkins et al 1995;Jiang et al 1997a, b). Among them, UV resistance 2 and 3 (uvr2 and uvr3) mutants are caused by mutations in cyclobutane pyrimidine (CPD)-and pyrimidine pyrimidone dimer (6-4 product)-specific photolyase genes, respectively (Ahmad et al 1997;Nakajima et al 1998). UV hypersensitive 1 and 3 (uvh1 and uvh3) mutants carry mutations in the endonuclease genes for nucleotide excision repair, which have been isolated by a positional cloning strategy (Liu et al , 2001.…”

Section: Introductionmentioning

confidence: 99%

Delimitation of the chromosomal region for a quantitative trait locus, qUVR-10, conferring resistance to ultraviolet-B radiation in rice (Oryza sativa L.)

et al. 2003

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Wide variation in ultraviolet-B (UVB) resistance is observed among rice varieties. In a previous study, three quantitative trait loci (QTLs) controlling UVB resistance were detected by QTL analysis, using backcross inbred lines (BILs) derived from a cross between a japonica cultivar, 'Nipponbare', and an indica cultivar, 'Kasalath'. Among them, qUVR- 10, a QTL for UVB resistance on chromosome 10, showed the largest effect. Plants homozygous for the Nipponbare allele at qUVR- 10 were resistant to UVB, unlike those homozygous for the Kasalath allele. To determine more precisely the chromosomal location of qUVR- 10, we performed a linkage mapping of qUVR- 10 as a single Mendelian factor using advanced backcross progeny. Advanced progeny testing of F(4) families enabled us to determine the genotype classes of the qUVR- 10 locus with high reliability. As a result, qUVR- 10 was mapped between RFLP markers C60755S and C1757S, and co-segregated with C913A. In addition, a sequence showing high similarity to the Arabidopsis cyclobutane pyrimidine dimer (CPD) photolyase gene, which has been found to be involved in sensitivity to UV radiation in Arabidopsis and rice, was mapped in the candidate genomic region of qUVR- 10. This result suggests that the CPD photolyase gene is a positional candidate for qUVR- 10.

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An enzyme similar to animal type II photolyases mediates photoreactivation in Arabidopsis.

Cited by 121 publications

References 37 publications

An unidentified ultraviolet-B-specific photoreceptor mediates transcriptional activation of the cyclobutane pyrimidine dimer photolyase gene in plants

An unidentified ultraviolet-B-specific photoreceptor mediates transcriptional activation of the cyclobutane pyrimidine dimer photolyase gene in plants

Increased DNA repair in Arabidopsis plants overexpressing CPD photolyase

Delimitation of the chromosomal region for a quantitative trait locus, qUVR-10, conferring resistance to ultraviolet-B radiation in rice (Oryza sativa L.)

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