An enzymic approach to the analysis of microbial activity during plant litter decomposition

Sinsabaugh, Robert L.; Antibus, Robert K.; Linkins, Arthur E.

doi:10.1016/0167-8809(91)90092-c

Cited by 277 publications

(138 citation statements)

References 34 publications

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“…Enzymes were chosen based on their metabolic function and use in previous studies (Table 2; Sinsabaugh et al, 1991Sinsabaugh et al, , 2008. Enzyme assays were performed as follows: 10 g of sediment sample was placed in 300 ml of acetate buffer (32.1 mM sodium acetate, 0.1% glacial acetic acid, pH 5, autoclaved).…”

Section: Study Sitementioning

confidence: 99%

“…Approximately 3 g of soil or sediment was preserved in 10 ml of 2% formalin containing 0.1% pyrophosphate and stored at 4 1C for determination of bacterial abundance. Subsamples for measuring potential enzyme activities were frozen at À20 1C as recommended by Sinsabaugh et al (1991) in case immediate analyses are not possible. Subsamples for molecular analyses were frozen in liquid nitrogen and stored at À80 1C until DNA was extracted.…”

Section: Study Sitementioning

confidence: 99%

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Disconnect of microbial structure and function: enzyme activities and bacterial communities in nascent stream corridors

et al. 2011

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A fundamental issue in microbial and general ecology is the question to what extent environmental conditions dictate the structure of communities and the linkages with functional properties of ecosystems (that is, ecosystem function). We approached this question by taking advantage of environmental gradients established in soil and sediments of small stream corridors in a recently created, early successional catchment. Specifically, we determined spatial and temporal patterns of bacterial community structure and their linkages with potential microbial enzyme activities along the hydrological flow paths of the catchment. Soil and sediments were sampled in a total of 15 sites on four occasions spread throughout a year. Denaturing gradient gel electrophoresis (DGGE) was used to characterize bacterial communities, and substrate analogs linked to fluorescent molecules served to track 10 different enzymes as specific measures of ecosystem function. Potential enzyme activities varied little among sites, despite contrasting environmental conditions, especially in terms of water availability. Temporal changes, in contrast, were pronounced and remarkably variable among the enzymes tested. This suggests much greater importance of temporal dynamics than spatial heterogeneity in affecting specific ecosystem functions. Most strikingly, bacterial community structure revealed neither temporal nor spatial patterns. The resulting disconnect between bacterial community structure and potential enzyme activities indicates high functional redundancy within microbial communities even in the physically and biologically simplified stream corridors of early successional landscapes.

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Section: Study Sitementioning

confidence: 99%

Section: Study Sitementioning

confidence: 99%

Disconnect of microbial structure and function: enzyme activities and bacterial communities in nascent stream corridors

et al. 2011

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“…CMCase is an endo-β-glucanase (EC 3.2.1.4), that is used as an index of microbial activity in cellulosic material decomposition [27]. CMCase activity ranged from 4.5 to 24.0 g-glucose d −1 per square meter in the soil from 0-15 cm depth (Tab.…”

Section: Soil Enzyme Activitiesmentioning

confidence: 99%

Article

et al. 2006

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-The CO 2 fluxes from the forest floor were measured using a closed chamber method at 26 sites from 26 • N Lat. to 44 • N Lat. in Japan. Seasonal fluctuation in CO 2 flux was found to correlate exponentially with seasonal fluctuation in soil temperature at each site. Estimate of annual carbon emission from the forest floor ranged from 3.1 to 10.6 Mg C ha −1 . The emission rate of soil-organic-carbon-derived CO 2 , obtained by incubation of intact soil samples, correlated closely with the carboxymethylcellulase (CMCase) activity in the soil. The sum of cool-water soluble polysaccharides, hot-water soluble polysaccharides, hemicellulose, and cellulose content in the soil was greater at the sites with low CMCase activity than that at the sites with high CMCase activity. Because the sites in cooler-climate sites had a high content of easily decomposable soil organic carbon and organic litter, the potential increase in CO 2 efflux from forest floor with increasing soil temperature would be greater in cooler-climate sites.cellulose / Japanese forest / soil organic carbon / soil respiration Résumé -Fort potentiel d'accroissement de flux de CO 2 issu de la surface du sol forestier en relation avec le réchauffement global dans les régions fraîches du Japon. Le flux de CO 2 issu du sol forestier a été mesuré dans 26 sites, allant du 26 • au 44 • de latitude Nord dans l'archipel japonais, en utilisant la méthode des chambres fermées. Il a pu être mis en évidence que la fluctuation saisonnière du flux de CO 2 était corrélée de façon exponentielle avec celle de la température du sol de chacun des sites étudiés. L'estimation annelle de l'émission de carbone venant du sol variait de 3,1 à 10,6 Mg C ha −1 . Le taux d'émission de CO 2 obtenu par incubation d'échantillons intacts de sol est corrélé positivement avec l'activité de la carboxyméthacellulase (CMCase), dans le sol. La somme totale des polysaccharides solubles dans l'eau froide, des polysaccharides solubles dans l'eau chaude, des hémicelluloses et de la cellulose contenus dans le sol était plus grande dans les sites caractérisés par une faible activité CMCase que dans les sites avec une forte activité CMCase. Du fait que les sites en climat frais ont un contenu élevé en carbone organique du sol facilement décomposable et une litière organique, le potentiel d'accroissement du flux de CO 2 avec l'accroissement de la température du sol devrait être plus grand dans les sites à climat frais.cellulose / forêt japonaise / carbone organique du sol / respiration du sol

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“…This enzyme catalyses the hydrolysis of glucosides. Cellulose is quantitatively the most important organic compound in the biosphere; consequently, the product of its enzymatic hydrolysis is important as an energy source for soil microorganisms, being of significant importance in the C cycle (Sinsabaugh et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Effect of deadwood of different tree species in various stages of decomposition on biochemical soil properties and carbon storage

Błońska

Kacprzyk

Spólnik

2017

Ecological Research

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The primary objective of this paper was to estimate how the mass of Silver fir (Abies alba Mill.) and Scots pine (Pinus sylvestris L.) deadwood in two decay classes affected biochemical processes and the accumulation of soil organic matter, as well as the extent of this impact. We evaluated deadwood mass, as well as the biological activity and influence of the distance from deadwood on biological activity and carbon (C) storage. The investigation was carried out in Magurski National Park, southern Poland, in four randomly selected study plots. The organic C and nitrogen contents and soil pH and texture were analysed. The study also included the determination of enzyme activities. The results obtained demonstrated that soil C storage and the biological activity of the soil were influenced by deadwood form, tree species and decay class. Significant differences in the C content in the soil between the logs of fir and pine trees were noted. With the advancement of the deadwood decomposition process, the C concentration and enzyme activity increased slightly. Lower C accumulation occurred further away from the deadwood. At a 100-cm distance from the deadwood, the influence of the logs was not evident. Higher C storage was found in the soil around the stumps than in the decaying logs. This study confirmed an important role of deadwood in forest ecosystems, i.e., maintaining species biodiversity and increasing C accumulation in the soil environment with a simultaneous increase in the biological activity of the soil.

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An enzymic approach to the analysis of microbial activity during plant litter decomposition

Cited by 277 publications

References 34 publications

Disconnect of microbial structure and function: enzyme activities and bacterial communities in nascent stream corridors

Disconnect of microbial structure and function: enzyme activities and bacterial communities in nascent stream corridors

Article

Effect of deadwood of different tree species in various stages of decomposition on biochemical soil properties and carbon storage

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