An enzymic bicarbonate reagent that is free of pyruvate interference.

Peled, Nina

doi:10.1093/clinchem/27.1.199

Cited by 7 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pyruvate in the sample may be endogenous produced in vivo or produced by decarboxylation of oxaloacetate to pyruvate. 4 Increased LD would accentuate the decarboxylation of oxaloacetate to pyruvate.…”

Section: Resultsmentioning

confidence: 99%

Artifactually elevated serum bicarbonate results caused by elevated serum lactate dehydrogenase concentrations

et al. 2013

View full text Add to dashboard Cite

Background: An accurate estimation of serum bicarbonate concentration (HCO À 3 ) is essential to the diagnosis and treatment of acid-base disorders and electrolyte disturbances. We noted significant discrepancies between HCO À 3 concentration measured by the Olympus AU2700 analyser and total serum carbon dioxide (TCO 2 ) concentration derived from a Radiometer blood gas analyser on several patient samples. This was reported to the manufacturer which led to a recall of certain reagent lot numbers. We hypothesised the mechanism for this interference to be elevated levels of lactate dehyrogenase (LD). Methods: We investigated the effect of increasing LD concentration on HCO À 3 with the reagent lot that was recalled and compared this with a subsequent reagent lot that was known to be unaffected on an Olympus AU2700. Results: The experimental data confirmed a positive interference in the Olympus AU2700 HCO À 3 assay using older reagent lot numbers. The false positive HCO À 3 interference was significant (>10% change) when the LD concentration exceeded 845 U/L. Conclusions: Very high levels of LD concentrations were the cause of interference in the Olympus AU2700 HCO À 3 enzymatic assay. Laboratorians should be aware that very high levels of LD may be a potential interference in some enzymatic HCO À 3 assays.

show abstract

Section: Resultsmentioning

confidence: 99%

Artifactually elevated serum bicarbonate results caused by elevated serum lactate dehydrogenase concentrations

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Bicarbonate concentrations were determined enzymatically using the method of Peled (1981) adapted for 96‐well plates. Two hundred and fifty microlitres of reaction mixture was preincubated at 37 °C and added to each well.…”

Section: Methodsmentioning

confidence: 99%

Bicarbonate gradients modulate growth and colony morphology inAspergillus nidulans

Rodriguez-Urra¹,

Jiménez²,

Dueñas³

et al. 2009

FEMS Microbiology Letters

View full text Add to dashboard Cite

Fungal colonies expand through apically extending peripheral hyphae. These long and relatively unbranched leaders leave behind cells that show a higher degree of branching and lower growth rates. Towards the centre of the colony, quiescent, but viable cells sustain processes such as transport of water, solutes and organelles, and support the concerted genesis of specialized reproductive structures. A survey of chemical signals controlling changes in hyphal growth and branching of Aspergillus nidulans in solid minimal medium revealed that progressive accumulation of bicarbonate from the periphery to the centre of the colony effected a dual modulation of both parameters. At areas immediately behind the periphery, bicarbonate induced progressive branching without a significant change in the specific growth rate. At distal and central regions, increased branching was accompanied by progressive inhibition of the specific growth rate. The results provide new evidence that chemical cues participate in the control of colony morphology and function, which are likely to vary with changing environmental conditions.

show abstract