An Epitope‐Imprinted Biointerface with Dynamic Bioactivity for Modulating Cell–Biomaterial Interactions

Abstract

In this study, an epitope‐imprinting strategy was employed for the dynamic display of bioactive ligands on a material interface. An imprinted surface was initially designed to exhibit specific affinity towards a short peptide (i.e., the epitope). This surface was subsequently used to anchor an epitope‐tagged cell‐adhesive peptide ligand (RGD: Arg‐Gly‐Asp). Owing to reversible epitope‐binding affinity, ligand presentation and thereby cell adhesion could be controlled. As compared to current strategies for the fabrication of dynamic biointerfaces, for example, through reversible covalent or host–guest interactions, such a molecularly tunable dynamic system based on a surface‐imprinting process may unlock new applications in in situ cell biology, diagnostics, and regenerative medicine.

Epitope Imprinting Technology: Progress, Applications, and Perspectives toward Artificial Antibodies

“…Pan et al recently employed epitope‐imprinted materials for the modulation of dynamic cell adhesion, as shown in Figure . A structurally symmetrical and carboxyl‐rich peptide (DDDGGDDD) was applied as the template for constrained epitope surface imprinting.…”

“…Schematic illustration of the epitope‐imprinted biointerface used for the modulation of dynamic cell adhesion. Reproduced with permission . Copyright 2017, Wiley‐VCH.…”

Chemical Antibody Mimics Inhibit Cadherin‐Mediated Cell–Cell Adhesion: A Promising Strategy for Cancer Therapy

“…MIPs were first described in the 1970–80s, but it is only in the past few years that their potential in cell biology research and nanomedicine applications has emerged. Indeed MIPs templated with monosaccharides, peptides, and proteins have been shown to interact with cells and even regulate cell behaviors, thanks to the production of nanosized biocompatible polymers with high affinity. In this work, we took advantage of the exposed N‐terminal part of cadherins (Supporting Information, Figure S2) to create a MIP that selectively recognizes a relevant epitope in classical type I cadherins.…”

Chemical Antibody Mimics Inhibit Cadherin‐Mediated Cell–Cell Adhesion: A Promising Strategy for Cancer Therapy

“…MIPs were first described in the 1970–80s, but it is only in the past few years that their potential in cell biology research and nanomedicine applications has emerged. Indeed MIPs templated with monosaccharides, peptides, and proteins have been shown to interact with cells and even regulate cell behaviors, thanks to the production of nanosized biocompatible polymers with high affinity. In this work, we took advantage of the exposed N‐terminal part of cadherins (Supporting Information, Figure S2) to create a MIP that selectively recognizes a relevant epitope in classical type I cadherins.…”