An Escherichia coli mutant unable to support site-specific recombination of bacteriophage λ

Kikuchi, Akihiko; Flamm, Eric L.; Weisberg, Robert A.

doi:10.1016/0022-2836(85)90207-4

Cited by 59 publications

(35 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As mentioned earlier, in vivo recombination between wild-type attL sites is efficient when IHF is present (Kikuchi et al 1985). If this recombination is mimicked by the in vitro bent-L pathway, we would expect the P 0 1 site to be dispensable for the in vivo reaction.…”

Section: Dispensability Of the P 0 1 Arm Binding Site For Ihf-stimulamentioning

confidence: 78%

**Architectural flexibility in lambda site‐specific recombination: three alternate conformations channel the attL site into three distinct pathways**

Segall

Nash

1996

Genes to Cells

View full text Add to dashboard Cite

Background: In the phage lambda life cycle, the Integrase (Int) protein carries out recombination between two different sets of DNA substrates: attP and attB in integration, attL and attR in excision. In each case, the partners are very different in structure from each other and the recombination reaction between them is effectively irreversible. For comparison, we have studied the recombination mediated by Int between two identical attL sites. Both in vitro and in vivo, recombination between two attL sites can be mediated inefficiently by Int alone. But, while IHF can stimulate recombination 5-10-fold in vivo (to the level of excision and integration), this stimulation is not observed under standard conditions in vitro.

show abstract

Section: Dispensability Of the P 0 1 Arm Binding Site For Ihf-stimulamentioning

confidence: 78%

**Architectural flexibility in lambda site‐specific recombination: three alternate conformations channel the attL site into three distinct pathways**

Segall

Nash

1996

Genes to Cells

View full text Add to dashboard Cite

show abstract

“…One was the discovery that a host component was required for the reaction (150). Purification of the extract revealed that the component was a single, heterodimeric, basic protein that was dubbed integration host factor (IHF) (151 (114,136,204). IHF, an accessory factor for many bacterial functions, has been co-opted by for sitespecific recombination, DNA packaging (see below), and other reactions.…”

Section: Prophage Insertionmentioning

confidence: 99%

Little Lambda, Who Made Thee?

Gottesman

Weisberg

2004

Microbiol Mol Biol Rev

Self Cite

View full text Add to dashboard Cite

SUMMARY The study of the bacteriophage λ has been critical to the discipline of molecular biology. It was the source of key discoveries in the mechanisms of, among other processes, gene regulation, recombination, and transcription initiation and termination. We trace here the events surrounding these findings and draw on the recollections of the participants. We show how a particular atmosphere of interactions among creative scientists yielded spectacular insights into how living things work.

show abstract

“…It has subsequently been shown to play a role in phage DNA packaging, plasmid maintenance, and bacterial gene expression but is not required for cell viability (15). IHF is a heterodimeric protein encoded by two genes, himA and hip (or himD), located at 38 and 25 min, respectively, on the E. coli chromosome (35,45). The subunits encoded by these genes, IHFot and IHFP, respectively, form a heterodimer that binds and bends DNA at specific sites (57,59,67).…”

mentioning

confidence: 99%