An essential carboxylic acid group in human prostate acid phosphatase

Saini, Mohan S.; Etten, Robert L. Van

doi:10.1016/0005-2744(79)90305-x

Cited by 35 publications

(12 citation statements)

References 20 publications

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“…(a) The assay procedure of Saini and Van-Etten (1979) were adopted with slight modifications for the assay of alkaline phosphatase (AlkPase) activity of liver homogenate using p-nitrophenyl disodium phosphate (2.5 mM) as the substrate in 25 mM Tris-Cl buffer pH 9.0 containing 5 mM MgCl 2 -6H 2 O. The activity was expressed as the moles of p-nitrophenol/mg protein/15 min at 37°C.…”

Section: (Ii) Enzyme Assaysmentioning

confidence: 99%

Studies on Bioactivity of the Root Extract of Plumbago zeylanica

Oyedapo

1996

International Journal of Pharmacognosy

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The phosphate buffered saline extract of the roots of Plumbago zeylanica was investigated for anti-inflammatory activity. The extract stabilized red blood cells subjected to both heat and hypotonic induced lyses. The extract exhibited a biphasic response. The enzymatic activities of both alkaline and acid phosphatases were reduced, while adenosine triphosphatase activity was stimulated in the liver homogenates of formaldehyde induced arthrititic rats. A possible anti-inflammatory action of the extract of P. zeylanica is, therefore, speculated. MATERIALS AND METHODS Plant MaterialFresh roots of P. zeylanica were collected during May and August at Babajakan Village, Osun-State, Nigeria. The plant was identified and authenticated by Dr. A.A. Jaiyeola of the Department of Botany of Obafemi Awolowo University. The herbarium specimen (Code Number OC.488) was deposited at the Herbarium (IFE). Preparation of Root Extract of P. zeylanicaAbout 25 g of air dried roots of P. zeylanica were homogenized in 250 ml of 0.2 M NaCl in 5 mM sodium phosphate buffer pH 7.2 and stirred at 4°C tor 24 hr. The suspension was filtered through cotton gauze followed by centrifugation at 5000 rpm for 20 min at room temperature and the supernatant freeze-dried. The yield of brownish residue was 12.5%. 2.5 g of the residue was dissolved in 100 ml of PBS for anti-inflammatory studies. AnimalsHealthy male and female Wistar rats (average body weights of 112 g) were kept in a laboratory environment for three weeks, fed with food pellets and watered ad libitum. Anti-inflammatory StudiesArthritis was induced in rats with formaldehyde solution using the method described by Brownlee (1950). Formaldehyde (26.6 µM in 0.1 ml) was injected subcutaneously into each footpad on the first and third days, respectively. Rats were divided into four groups Pharmaceutical Biology Downloaded from informahealthcare.com by Nanyang Technological University on 08/24/15For personal use only.

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Section: (Ii) Enzyme Assaysmentioning

confidence: 99%

Studies on Bioactivity of the Root Extract of Plumbago zeylanica

Oyedapo

1996

International Journal of Pharmacognosy

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“…Electrophoresis in polyacrylamide gels was performed at 4°C as previously described [5] but with the high pH buffer at pH 8.5, whereas the low pH gels (7% monomer concentration) were run at pH 3.8 in 0.188 M acetate-glycine buffer.…”

Section: Electrophoresismentioning

confidence: 99%

“…of the lysosome concept [3]. Assays for HPAP activity are of importance in forensic chemistry [4] and studies on the enzyme mechanism of action of HPAP remain of interest [5]. The methods used for the assay of the enzyme in serum include catalytic activity [6], electrophoresis [7], ion-exchange chromatography [8], and immunochemical techniques [9-1 I].…”

Section: Introductionmentioning

confidence: 99%

Isolation, characterization, and spontaneous interconversion of two forms of human prostatic acid phosphatase

McTigue¹,

Etten²

1982

The Prostate

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Starting with human prostatic acid phosphatase (HPAP) purified by affinity chromatography on Sepharose-bound N-(6-aminohexyl)tartramic acid [Van Etten, R. L. and Saini, M. S. (1978) Clin. Chem. 24, 1525], two forms of the enzyme can be separated by gradient elution DEAE-cellulose chromatography. The two enzyme forms are electrophoretically distinguishable on high pH gels. Under some conditions of storage one of these forms undergoes a conversion to the other. Both forms have identical amino acid compositions and Vmax values with typical substrates, but differences are observed in circular dichroism spectra, relative fluorescence intensities, Km values, and carbohydrate composition. It is concluded that the two forms differ only in the structure of their (nonphosphorylated) carbohydrate side chains. In view of the possible interconversion of the two forms upon storage, caution must be observed in any attempt to attach clinical significance to the relative amounts of the two forms.

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“…The assays of acid and alkaline phosphatases activities were carried out according to the method of Saini and Van Etten (1979) as slightly modified…”

Section: Biochemical Analysesmentioning

confidence: 99%

Protective effects of extract and fraction of root- bark of Garcinia kola (Heckel) on the renal biochemical parameters of gentamicin-induced nephrotoxic rats

Komolafe¹,

Akinlalu²,

Ogunsusi³

et al. 2016

Afr. J. Biochem. Res.

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The study evaluated the nephroprotective potentials of methanolic extract (ME) and dichloromethane fraction (DCMF) of root-bark of Garcinia kola on gentamicin-induced nephrotoxicity in rats with a view to utilize the extracts of the root of G. Kola in the amelioration of antibiotic-induced kidney damage. The study involved preparation of the extract, fractionation, phytochemical screening and evaluation of phenolics and flavonoid contents. Moreover, both the acute and sub-acute toxicities of ME and DCMF were determined in mice and albino rats with concentrations ranging from 10 to 5000 mg/kg body weight (bwt) respectively. Forty albino rats were divided into 8 groups of five animals each and induced with gentamicin (80 mg/kg bwt). The renal biochemical metabolites and enzymes were quantified and assayed respectively. The histopathological examinations of kidney were carried out. Phytochemical screening of the ME and DCMF of the root-bark of G. kola revealed the presence of flavonoids, tannins, cardiac glycosides, saponins, alkaloids and terpenoids. The extract and DCMF did not elicit any adverse effect and a dose of 250 mg/kg bwt of ME and DCMF was selected for further studies. The administration of gentamicin (80 mg/kg bwt) caused elevated levels of plasma renal biomarkers, reduction in AciPase activities. Moreover, administration of gentamicin (80 mg/kg bwt) resulted in damage to kidney structures. The treatments (pre and post) of the rats with 250 mg/kg extract and fraction reversed alterations of biochemical parameters which were supported by low levels of tubular and glomerular injuries induced by gentamicin treatment. The study concluded that, both the extract and fraction of G. kola root-bark elicited antioxidant and appreciable nephroprotective potentials.

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An essential carboxylic acid group in human prostate acid phosphatase

Cited by 35 publications

References 20 publications

Studies on Bioactivity of the Root Extract of Plumbago zeylanica

Studies on Bioactivity of the Root Extract of Plumbago zeylanica

Isolation, characterization, and spontaneous interconversion of two forms of human prostatic acid phosphatase

Protective effects of extract and fraction of root- bark of Garcinia kola (Heckel) on the renal biochemical parameters of gentamicin-induced nephrotoxic rats

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