An Esterase from Anaerobic <i>Clostridium hathewayi</i> Can Hydrolyze Aliphatic–Aromatic Polyesters

Perz, Veronika; Hromic, Altijana; Baumschlager, Armin; Steinkellner, Georg; Pavkov‐Keller, Tea; Gruber, Karl; Bleymaier, Klaus; Zitzenbacher, Sabine; Zankel, Armin; Mayrhofer, Claudia; Sinkel, Carsten; Kueper, Ulf; Schlegel, Katharina; Ribitsch, Doris; Guebitz, Georg M.

doi:10.1021/acs.est.5b04346

Cited by 46 publications

(37 citation statements)

References 49 publications

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“…EstC5 shows the typical triad Ser-Asp-His, while EstC9 may utilize glutamic acid (Ser-Glu-His) instead of aspartic acid. To compare the affiliations of the metagenome esterases with those of previously characterized polyesterases with documented activity toward PBAT, the following enzymes were included in the phylogenetic analysis: esterases Cbotu_EstA, Cbotu_EstB (Clostridium botulinum) (20), and Chath_Est1 (Clostridium hathewayi) (21), lipase Pfl1 (Pelosinus fermentans) (44), and cutinases Cut190 (Saccharomonosporas viridis) (19) and Thc_Cut1 (Thermobifida cellulosilytica) (45). These six known PBAT hydrolases share the same catalytic triad Ser-Asp-His (or Ser-Glu-His, in the case of Chath_Est1).…”

Section: Resultsmentioning

confidence: 99%

“…The cutinase Thc_ Cut1 shows greater specificity for the hydrolysis of aromatic ester bonds (Ta-B) than does the cutinase HiC (12). Moreover, the polyesterase activities of the novel enzymes EstB3 and EstC7 with milled PBAT were significantly higher than the activities of the recently presented esterases from the anaerobic bacteria Clostridium hathewayi (Chath_Est1) (21) and Clostridium botulinum (Cbotu_Est and Cbotu_EstB) (20).…”

Section: Fig 6 Hydrolysis Of Milled Pbat (A) and Pbat Foil (B) By Purmentioning

confidence: 95%

“…and from fungi (e.g., Fusarium solani, Humicola insolens, Aspergillus oryzae, and Rhizopus spp.) (6,11,(16)(17)(18)(19)(20)(21). While searching for new microorganisms, Yoshida et al recently discovered the novel bacterium Ideonella sakaiensis, which contains two PET hydrolases and is capable of utilizing PET as an energy and carbon source (22).…”

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confidence: 99%

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Discovery of Polyesterases from Moss-Associated Microorganisms

Müller

Perz

Provasnek

et al. 2017

Appl Environ Microbiol

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The growing pollution of the environment with plastic debris is a global threat which urgently requires biotechnological solutions. Enzymatic recycling not only prevents pollution but also would allow recovery of valuable building blocks. Therefore, we explored the existence of microbial polyesterases in microbial communities associated with the Sphagnum magellanicum moss, a key species within unexploited bog ecosystems. This resulted in the identification of six novel esterases, which were isolated, cloned, and heterologously expressed in Escherichia coli. The esterases were found to hydrolyze the copolyester poly(butylene adipate-co-butylene terephthalate) (PBAT) and the oligomeric model substrate bis[4-(benzoyloxy)butyl] terephthalate (BaBTaBBa). Two promising polyesterase candidates, EstB3 and EstC7, which clustered in family VIII of bacterial lipolytic enzymes, were purified and characterized using the soluble esterase substrate p-nitrophenyl butyrate (K m values of 46.5 and 3.4 M, temperature optima of 48°C and 50°C, and pH optima of 7.0 and 8.5, respectively). In particular, EstC7 showed outstanding activity and a strong preference for hydrolysis of the aromatic ester bond in PBAT. Our study highlights the potential of plant-associated microbiomes from extreme natural ecosystems as a source for novel hydrolytic enzymes hydrolyzing polymeric compounds.IMPORTANCE In this study, we describe the discovery and analysis of new enzymes from microbial communities associated with plants (moss). The recovered enzymes show the ability to hydrolyze not only common esterase substrates but also the synthetic polyester poly(butylene adipate-co-butylene terephthalate), which is a common material employed in biodegradable plastics. The widespread use of such synthetic polyesters in industry and society requires the development of new sustainable technological solutions for their recycling. The discovered enzymes have the potential to be used as catalysts for selective recovery of valuable building blocks from this material.

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Section: Resultsmentioning

confidence: 99%

Section: Fig 6 Hydrolysis Of Milled Pbat (A) and Pbat Foil (B) By Purmentioning

confidence: 95%

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Discovery of Polyesterases from Moss-Associated Microorganisms

Müller

Perz

Provasnek

et al. 2017

Appl Environ Microbiol

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“…Hydrolysis samples with PLA and PBS were precipitated following the Carrez method (Perz et al, 2016b) and filtered through 0.20 µm Nylon filters (GVS, Indianapolis, United States). The analytes were separated by HPLC using refractive index detection (1100 series, Agilent Technologies, Palo Alto, CA, United States) equipped with an ICSep−ION−300 column (Transgenomic Organic, San Jose, CA, United States) of 300 mm by 7.8 mm and 7 µm particle diameter.…”

Section: High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

“…Due to its special barrier properties it is widely used for food packaging or organic waste bags. Its biodegradability by microorganisms and enzymes is well known, therefore it is widely used as raw material for compostable plastics (Perz et al, 2016b). PBAT is manufactured in industrial scale by several companies and can be used in combination with other polymers such as PLA to manufacture blended materials.…”

Section: Introductionmentioning

confidence: 99%

High Throughput Screening for New Fungal Polyester Hydrolyzing Enzymes

et al. 2020

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There is a strong need for novel and more efficient polyester hydrolyzing enzymes in order to enable the development of more environmentally friendly plastics recycling processes allowing the closure of the carbon cycle. In this work, a high throughput system on microplate scale was used to screen a high number of fungi for their ability to produce polyester-hydrolyzing enzymes. For induction of responsible enzymes, the fungi were cultivated in presence of aliphatic and aromatic polyesters [poly(1,4butylene adipate co terephthalate) (PBAT), poly(lactic acid) (PLA) and poly(1,4-butylene succinate) (PBS)], and the esterase activity in the culture supernatants was compared to the culture supernatants of fungi grown without polymers. The results indicate that the esterase activity of the culture supernatants was induced in about 10% of the tested fungi when grown with polyesters in the medium, as indicated by increased activity (to >50 mU/mL) toward the small model substrate para-nitrophenylbutyrate (pNPB). Incubation of these 50 active culture supernatants with different polyesters (PBAT, PLA, PBS) led to hydrolysis of at least one of the polymers according to liquid chromatography-based quantification of the hydrolysis products terephthalic acid, lactic acid and succinic acid, respectively. Interestingly, the specificities for the investigated polyesters varied among the supernatants of the different fungi.

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Hungatella

Sharma

Singh

Gundawar

et al. 2019

Bergey's Manual of Systematics of Archaea and Bacteria

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Hun.ga.tel'la. N.L. fem. dim. n. Hungatella , named in honor of R.E. Hungate, who contributed much to anaerobic bacteriology. Firmicutes / Clostridia / Clostridiales / Lachnospiraceae / Hungatella Hungatella is a genus classified in the phylum Firmicutes, class Clostridia , order Lachnospirales, and family Lachnospiraceae . Presently, two species are described in this genus, Hungatella hathewayi and Hungatella effluvii . Members of the genus have been isolated from diverse environments such as human blood, human feces, and an anaerobic effluent treatment plant. The type species of the genus, H. hathewayi is clinically important as several strains have been reported mostly from blood samples of patients. Cells are Gram‐stain‐variable, motile, obligate anaerobe, endospore‐forming, and rod‐shaped with length and width ranging from 2 to 5 µm and 0.7 to 1.5 µm. H. hathewayi has been reported to form slimy capsules. The fatty acid composition is dominated by unsaturated fatty acids and C 15:1 ω8c being the major fatty acid. Acetate and propionate are formed as a result of glucose fermentation. Total polar lipid profile is enriched by phosphatidylglycerol (PG) along with unidentified phospholipids and glycolipids. Comparative genomic and phenotypic analyses assigned the phylogenetically close relatives of the genus Hungatella to the family Lachnospiraceae . This family is comprised of thirty‐nine genera including the genus Hungatella . While two validly published species have been described in this genus ( H. hathewayi and H. effluvii ), there are some other strains pertaining to various species of the genus Clostridium that have also been described under this genus by Genome Taxonomy Database (GTDB). The DNA G + C content of the genus range between 42 and 49 mol%. DNA G + C content (mol%) : 42–49 ( T m values derived from whole genome sequence). Type species : Hungatella hathewayi (Steer, Collins, Gibson, Hippe and Lawson 2001) Kaur, Yawar, Kumar and Suresh 2014, 716 VP ( Clostridium hathewayi Steer, Collins, Gibson, Hippe and Lawson 2002, 685).

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An Esterase from Anaerobic Clostridium hathewayi Can Hydrolyze Aliphatic–Aromatic Polyesters

Cited by 46 publications

References 49 publications

Discovery of Polyesterases from Moss-Associated Microorganisms

Discovery of Polyesterases from Moss-Associated Microorganisms

High Throughput Screening for New Fungal Polyester Hydrolyzing Enzymes

Hungatella

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