An estrogen-responsive element derived from the 5′ flanking region of the Xenopus vitellogenin A2 gene functions in transfected human cells

Klein-Hitpaß, Ludger; Schorpp, Marina; Wagner, Ulrike; Ryffel, Gerhart U.

doi:10.1016/0092-8674(86)90705-1

Cited by 690 publications

(328 citation statements)

References 25 publications

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“…1). Sequence analysis between -1090 and +410 of a vtg gene did not reveal blocks of regulatory elements such as those described for the Xenopus and chicken genes [15,18]. No obvious similarity in the organization and sequence of the promoter region was observed between the rtvtg and other vertebrate vtg genes.…”

Section: The Promoter Of Rtvtg Genes Is Composed Almost Entirely Of Smentioning

confidence: 86%

“…Because of their remarkable E2-mediated stimulation, vtg genes have for long been the model of choice to decipher the molecular mechanisms of transcriptional regulation by ERs. Most of our knowledge of the basic mechanisms of transcriptional regulation is based on Xenopus laevis and chicken vtg genes [15][16][17][18]. Besides, the tilapia vtg gene promoter [19] was shown to contain several regions exhibiting more than 70% similarity with the X. laevis vtgA2 gene promoter.…”

Section: Introductionmentioning

confidence: 99%

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Control of vitellogenin genes expression by sequences derived from transposable elements in rainbow trout

Bouter

Buisine

Grand

et al. 2010

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

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In most of oviparous animals, vitellogenins (VTG) are the major egg yolk precursors. They are produced in the liver under the control of estrogens. In rainbow trout (Oncorhynchus mykiss), the vtg genes cluster contains an unusually large number of almost identical gene copies. In order to identify the regulatory elements in their promoters, we used a combination of reporter plasmids containing genomic sequences including putative estrogen response elements (EREs) and we performed transient transfection assays in MCF-7 and yeast cells.We found a functional ERE corresponding to the sequence GGGGCAnnnTAACCT (rtvtgERE), which differs from the consensus ERE (ERE cs ) by three base pairs. This nonpalindromic ERE is located in the env gene of a retrotransposon relic, 180 base pairs upstream of the transcriptional start site. Fluorescence anisotropy experiments confirmed that the purified human estrogen receptor α (hERα) can specifically bind to rtvtgERE. Furthermore, we observe that the stability of hERα-ERE cs and hERα-rtvtgERE complexes is similar with equilibrium dissociation constants of 3.0 nM and 6.2nM respectively, under our experimental conditions. Additionally, this rtvtgERE sequence displays a high E2-responsiveness through ER activation in cellulo.In the rainbow trout, the functional ERE (rtvtgERE) lies within promoter sequences which are mostly composed of sequences derived from transposable elements (TEs), which therefore may have acted as an evolutionary buffer to secure the proper expression of these genes.4

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Section: The Promoter Of Rtvtg Genes Is Composed Almost Entirely Of Smentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Control of vitellogenin genes expression by sequences derived from transposable elements in rainbow trout

Bouter

Buisine

Grand

et al. 2010

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

View full text Add to dashboard Cite

show abstract

“…The consensus ERE is defined as a palindromic 5 0 -GGTCA NNNTGACCC-3 0 sequence. 32 However, many well-characterized estrogen responsive genes exhibit imperfect homology to this consensus sequence. The ERE sequence homology introduced by the À179T exhibits perfect homology to an ERE half-site and significant palindromic, but imperfect, homology to the entire ERE motif.…”

Section: Discussionmentioning

confidence: 99%

An IFNG SNP with an estrogen-like response element selectively enhances promoter expression in peripheral but not lamina propria T cells

et al. 2006

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This study examines mucosa-specific regulatory pathways involved in modulation of interferon-g (IFN-g) in lamina propria T cells. Previous studies identified mucosa-specific CD2 cis-elements within the À204 to À108 bp IFNG promoter. Within this region, a single-site nucleotide polymorphism, À179G/T, imparts tumor necrosis factor-a stimulation of IFNG in peripheral blood lymphocytes, and is linked with accelerated AIDS progression. We discovered a putative estrogen response element (ERE) introduced by the À179T, which displays selective activation in peripheral blood mononuclear cells (PBMC) vs lamina propria mononuclear cells (LPMC). Transfection of PBMC with constructs containing the À179G or À179T site revealed CD2-mediated enhancement of the À179T compared to À179G allele, although, in LPMC, a similar level of expression was detected. Electrophoretic mobility shift assay (EMSA) analysis demonstrated CD2-mediated nucleoprotein binding to the À179T but not the À179G in PBMC. In LPMC, binding is constitutive to both À179G and À179T regions. Sequence and EMSA analysis suggests that the À179T allele creates an ERE-like binding site capable of binding recombinant estrogen receptor. Estrogen response element transactivation is enhanced by CD2 signaling, but inhibited by estrogen in PBMC but not in LPMC, although expression of estrogen receptor was similar. This is the first report to describe a potential molecular mechanism responsible for selectively controlling IFN-g production in LPMC.

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“…28 For RXRE(CRBPII)-SV-LUC, the RXRE (gatctgCTGTCAc AGGTCAc AGGTCAcAGGTCAcAGTT), 11 9 For RAR␣/VDR (Figure 2a), the VDR-ligand-binding domain (PCR-amplified (sequence verified) cDNA of amino acids 123 to 427 with engineered BamHI sites at either end) was inserted into the corresponding cloning site of pSG5-RAR␣ (cDNA for amino acids 1 to 199) immediately 3′ of the codon for RAR residue 199. For RAR␣/ER, the ligand-binding domain of ER (cDNA for amino acids 302 to 395) replaced that of VDR in pSG5-RAR␣/VDR.…”

Section: Plasmid Constructionmentioning

confidence: 99%

Characterization of the chimeric retinoic acid receptor RARα/VDR

et al. 1998

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An estrogen-responsive element derived from the 5′ flanking region of the Xenopus vitellogenin A2 gene functions in transfected human cells

Cited by 690 publications

References 25 publications

Control of vitellogenin genes expression by sequences derived from transposable elements in rainbow trout

Control of vitellogenin genes expression by sequences derived from transposable elements in rainbow trout

An IFNG SNP with an estrogen-like response element selectively enhances promoter expression in peripheral but not lamina propria T cells

Characterization of the chimeric retinoic acid receptor RARα/VDR

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