An etching based fluorescent probe for sensitive detection of hydrogen sulfide in cells

Abstract

Hydrogen sulfide (HS) is one of the most crucial gas signaling agents that mediate many physiological and pathological processes. However, rapid high-efficiency detection and imaging of HS in living cells is very challenging. Herein we reported a simple fluorescent nanoprobe using FAM-DNA/AgNP nanocomposites for fast and sensitive HS detection based on surface silver displacement. In contrast to the conventional principles for fluorescence turn-on analyte detection, the present work demonstrated a sensitive and selective AgNP based optosensor for the assay of HS. Compared with the majority of the reported HS probes, complex synthesis procedures and costly equipment are not involved in this assay.

Synthetic nanoprobes for biological hydrogen sulfide detection and imaging

“…[60,61] As far as H 2 S detection is concerned, AgNPs have been employed to a greater extent compared to AuNPs [62,63] as in vivo H 2 S readily reacts with silver (Ag) to produce silver sulfide (Ag 2 S). [64,65] This reaction is accompanied by a change in plasmon resonance wavelength and intensity, and is therefore used as the general detection mechanism principle. [66,67] It should be noted that copper nanoparticles (CuNPs), which are etched by sulfide in the presence of oxygen to form copper sulfide (CuS), have also been investigated as sensor candidates for H 2 S detection.…”

A Fluorescent Turn‐on Probe Based on Zn‐doped AgIn5S8 Quantum Dots for Imaging of Hydrogen Sulfide in Living Cells

“…Notably, bioimaging by staining with the fluorescent turn-on probe has been considered as an effective approach to detect H 2 S in vivo due to its visualization, high sensitivity and realtime spatiotemporal resolution ability. [9][10][11][12][13][14][15] To date, most studies about the fluorophores of such probes were focused on organic fluorescent molecules, while their poor water solubility, photobleaching and small Stokes shift may restrict them further application. Thus, it urgently needs to develop more suitable kind of fluorescent materials for H 2 S detection in vivo.…”

“…Traditional detection methods, including colorimetry, [6] chromatography, [7] electrochemical analysis etc ., [8] are usually not capable of real‐time monitoring H 2 S in living biological samples besides the deficiency of high‐cost, tedious pretreatment and tissues or cells destruction. Notably, bioimaging by staining with the fluorescent turn‐on probe has been considered as an effective approach to detect H 2 S in vivo due to its visualization, high sensitivity and real‐time spatiotemporal resolution ability [9–15] . To date, most studies about the fluorophores of such probes were focused on organic fluorescent molecules, while their poor water solubility, photobleaching and small Stokes shift may restrict them further application.…”