An evaluation and partial characterization of a bacteriocin produced by Lactococcus lactis subsp lactis ST1 isolated from goat milk

Taheri, Parastoo; Samadi, Nasrin; Ehsani, Mohammad Reza; Khoshayand, Mohammad Reza; Jamalifar, Hossein

doi:10.1590/s1517-83822012000400029

Cited by 19 publications

(9 citation statements)

References 22 publications

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“…For GTTP (also notified in Italy), no proof was found in the literature for the claimed ‘normalisation of dysbacteriosis’, associated with relief of diarrhoea in inflammatory bowel conditions according to the leaflet. Killed TP strains may have immunomodulatory effects by the release bacteriocins and anti-inflammatory agents41–43 and hence do not necessarily act as a barrier. Some TPs have been associated with increased diarrhoea and gastrointestinal symptoms in critically ill patients and in healthy newborns; an international panel assessing the safety of probiotics for human use concludes that ‘Products comprising heat-killed microbes should not be made available to infants until any adverse effects (AEs) from such products are better understood’ 44.…”

Section: Resultsmentioning

confidence: 99%

“…As mentioned, TPs may result in more diarrhoea and AEs, calling for well-controlled studies on GTTP 41 44. Concerns during heating include enhanced bacteriocin production (potentially >7 fold),75 and rapid lysis of dead bacteria on exposure to digestive fluids,76 potentially inducing effects beyond those established for viable strains 41 44. In the USA, TPs are only FDA approved if supported by extensive toxicity and tolerability studies 77 78…”

Section: Resultsmentioning

confidence: 99%

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Medical devices that look like medicines: safety and regulatory concerns for children in Europe

Huijghebaert

Bruyne²,

Allegaert³

et al. 2019

Arch Dis Child

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IntroductionMedical devices (MedDevs) and medicines are assessed (and monitored) differently before and after launch. There are products for repeated oral ingestion that are marketed in the European Union as MedDevs.Objectives and methodsTo illustrate the consequences of these differences in assessment, we compared the leaflet information of three MedDevs with the standards for medicines and with published evidence at launch. As examples, gelatin tannate (GT), its combination with tyndalised probiotics (TP) (GTTP) for diarrhoea and a gel containing hyaluronic acid (HA)/chondroitin sulfate (CS)/poloxamer (Pol407) (HACSPol) for gastro-oesophageal reflux disease were examined.ResultsApplying standards for medicines, product composition is insufficiently defined in the MedDev leaflet (eg, plant origin, polymerisation grade, dose and ratio of the relevant constituents). As no age limit is mentioned in the leaflets, all 3 products allow use in children from birth onwards, although published clinical documentation in children was poor (GT) or lacking (GTTP and HACSPol). MedDev leaflets do not mention adverse events (AEs), while literature search suggests safety concerns such as tannic acid (TA) cytotoxicity, potentially more diarrhoea/AEs with TP, use of doses higher than established safe (TA and HA) and lack of chronic toxicity studies for oral Pol407. None refers to interactions with medicines, although some ingredients may affect medicine absorption.ConclusionAlthough these MedDevs require repeated oral intake as do medicines, their assessment and monitoring differ significantly from the standards for medicines. Compared with medicines, MedDevs for repeated oral use are poorly labelled and rely on very limited clinical information at market release.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Medical devices that look like medicines: safety and regulatory concerns for children in Europe

Huijghebaert

Bruyne²,

Allegaert³

et al. 2019

Arch Dis Child

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“…This result is similar to Himelbloom et al (2001) that different concentration of inoculum had no significant effect on the production of bacteriocin by L. acidophilus LF221 and Candida piscicola A9b. On the other hand, Taheri et al (2012) studied that inoculum level significantly affected bacteriocin production by L. plantarum ST1. Maximum bacteriocin activity and stability (640 AU/ml) were obtained at the lowest inoculum level (0.01%).…”

Section: Discussionmentioning

confidence: 99%

Medium Optimization for Antimicrobial Production By Newly Screened Lactic Acid Bacteria

Rohmatussolihat¹,

Lisdiyanti²,

Yopi³

et al. 2018

Ann. Bogor.

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Lactic acid bacteria (LAB) are important for prevention of spoilage and pathogenic bacterial growth in foods due to their ability to generate antimicrobial substances. The objective of this study was to screen LAB for antimicrobial activity and to optimize culture medium for antimicrobial production using Response Surface Methodology (RSM) with Central Composite Design (CCD). Optimization of antimicrobial production of selected LAB was conducted with different combinations of glucose, NaCl, inoculum, and temperature. Our experimental results showed that from 129 LAB isolates, 55 showed significant inhibition against Bacillus subtilis, Escherichia coli, Micrococcus luteus, Staphylococcus aureus, Aspergillus niger, and Candida albicans. No isolates inhibited the growth of Aspergillus flavus. Lactobacillus plantarum LIPI13-2-LAB011 was selected for further study on culture medium optimization to inhibit the growth of C. albicans. From statistical analysis, the production of antimicrobial substances was significantly influenced by temperature, NaCl, and concentration of glucose. Furthermore, the optimum concentrations of glucose, concentration of inoculum, temperature, and NaCl were 1.63 %, 3.03%, 33.74°C, and 3.4%, respectively, with a maximum predicted inhibition index of 1.916, which increased 3.56-fold compared to that obtained in medium before optimization processes. The result was confirmed as when the optimum concentration of nutritions used, the inhibition index increased 3.12-fold.

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“…This is might be due to the presence of certain component in MRS. Previous findings reported that Tween 80 promoted bacteriocin release from various adsorbents Coventry et al 1996;Amiali et al 1998;Taheri et al 2012). In MRS, Tween 80 might cause bacteriocin S20 loosely adhered on the cell membrane and hence, easier to be released in acidic pH.…”

Section: Ph-mediated Adsorption and Extraction Of Bacteriocin S20mentioning

confidence: 94%

Characterization and the influence of milk solids-not-fat on the bacteriocin produced by Lactococcus lactis subsp. lactis S20 isolated from Chinese sauerkraut, a traditional fermented vegetable

et al. 2015

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Lactococcus lactis subsp. lactis S20, a bacteriocinogenic lactic acid bacterium, was isolated from Chinese sauerkraut. This strain produced the highest bacteriocin activity (1280 AU/ml) against Listeria monocytogenes ATCC 19115 after 20 h of culture at 30°C in MRS broth, although our results demonstrate that TSB was a suitable alternative bacteriocin production medium. Bacteriocin S20 exhibited inhibitory activity against most Gram-positive food pathogens but did not inhibit the growth of Gram-negative bacteria. Results of the stability tests indicate that bacteriocin S20 was resistant to trypsin, amylase, and lipase but sensitive to proteinase K, α-chymotrypsin, and pepsin. This bacteriocin was stable over a wide range of pH (pH 3-9) and at temperatures of up to 70°C for 15 min. The bacteriocin activity of bacteriocin S20 dropped to 40 AU/ml after autoclaving at 121°C for 15 min. Taken together, the results from the stability tests and molecular studies suggest that bacteriocin S20 is closely related to nisin A. Based on the bacteriocin adsorption study, pH 6 and pH 7 were the optimal pH for the adsorption of bacteriocin S20. The cells of the sensitive strain showed a higher adsorption of bacteriocin S20 than did the producer strain. However, adsorption to the resistant strain observed in this study suggests that bacteriocin S20 bound randomly to the cell membrane. Bacteriocin adsorption in the general medium TSB was relatively higher than that in MRS. Conversely, a higher recovery yield was observed in bacteriocin S20 extracted from MRS broth. In addition, bacteriocin S20 showed an effective antilisterial activity in skim milk containing <12 % (w/v) milk solids-not-fat.

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An evaluation and partial characterization of a bacteriocin produced by Lactococcus lactis subsp lactis ST1 isolated from goat milk

Cited by 19 publications

References 22 publications

Medical devices that look like medicines: safety and regulatory concerns for children in Europe

Medical devices that look like medicines: safety and regulatory concerns for children in Europe

Medium Optimization for Antimicrobial Production By Newly Screened Lactic Acid Bacteria

Characterization and the influence of milk solids-not-fat on the bacteriocin produced by Lactococcus lactis subsp. lactis S20 isolated from Chinese sauerkraut, a traditional fermented vegetable

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