An evaluation of a 24 hour malaria mobile case reporting system compared to the paper-based case reporting system in South Africa, 2015

Abstract

The isolates were sub cultured, DNA isolated and Sanger sequencing performed on the amplified PCR product. Blast n was performed at different score ad E-value parameters for all positive sequences against the whole NCBI nr/nt data base. Molecular phylogentic analysis with various serogroups of N.meningitides was performed by using MEGA 6 software package. Results: Of the 274 nasopharyngeal swabs, 10 (3.6%) grew Neisseria. DNA isolation and Sanger sequencing was performed on the amplified PCR product and sequence analysis was carried out. Blast analysis of all sequenced samples was performed against the whole NCBI-nr/nt database and within the Dataset. On molecular testing and sequence analysis, 4 of the samples were found to be N. meningitidis whereas one had close similarity to N. meningitidis. Only 2 students reported history of intimate kissing in the past 2 weeks and one had a history of using antibiotics. The isolates on Blast and molecular phlogeny analysis bore homology to serogroup 'B (Fig 1). Evolutionary relationship of N.meningitidis with different published serogroups Conclusion: Neisseria meningitides is seen in college freshmen and the potential implications for spread in close shared settings call for appropriate infection control measures. NCBI Blast hits of Sequence ID (Skimsmen0010) against whole NCBI (nr/nt) Data base.