An evaluation of a water purification system for use in animal facilities

Raynor, T. H.; White, Earl L.; Cheplen, J. M.; Sherrill, John; Hamm, Thomas E.

doi:10.1258/002367784780864983

Cited by 15 publications

(5 citation statements)

References 2 publications

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“…They had negative virus titers in the standard murine virus antibody determination tests (Microbiological Associates, Bethesda, MD). Rats were fed N1H 07 diet (Ziegler Bros., Gardner, PA) and tap water purified using a nanopure system (Raynor et al, 1982) ad libitum.…”

Section: Methodsmentioning

confidence: 99%

Ultrastructural characterization of the nasal respiratory epithelium in the rat

Monteiro‐Riviere

Popp

1984

Am. J. Anat.

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This ultrastructural study of the respiratory epithelium of the rat nasal mucosa revealed six morphologically distinct cell types: goblet cells, basal cells, ciliated cells, nonciliated columnar cells, cuboidal cells, and brush cells. The latter three have not been previously characterized in the rat nasal mucosa by transmission electron microscopy. Cuboidal cells observed on the conchae and lateral wall had short apical microvilli which were less dense than the microvilli of the nonciliated columnar cells. Nonciliated columnar cells also identified on the conchae and lateral wall had short microvilli and an extensive network of smooth endoplasmic reticulum in the apical region. The brush cell had distinct ultrastructural features; it was pear-shaped, with the broad base adjacent to the basement membrane and large microvilli on the surface. Microfilaments, microtubules, vesicles, and paired cisternae were found in the apical cytoplasm. Brush cells occurred singly on the conchae and lateral wall but were not identified in the respiratory epithelium of the nasal septum. These findings indicate the complexity of cell types composing the rat nasal respiratory epithelium.

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Section: Methodsmentioning

confidence: 99%

Ultrastructural characterization of the nasal respiratory epithelium in the rat

Monteiro‐Riviere

Popp

1984

Am. J. Anat.

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“…Sentinel animals from each group received were demonstrated to be free of known rodent pathogens by a standard screening necropsy and a standard rat murine viral antibody screening procedure (Microbiological Associates, Bethesda, MD). The rats were fed NIH07 diet (Ziegler Bros., Gardner, PA), and purified tap water ad libitum (Raynor et al, 1982). The nasal cavities were fixed by intravascular perfusion of aldehyde fixative and subsequently decalcified as previously described (Popp and Martin, 1981).…”

mentioning

confidence: 99%

Surface topography and distribution of cell types in the rat nasal respiratory epithelium: Scanning electron microscopic observations

Popp

Martin

1984

Am. J. Anat.

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Several cell types were identified in the rat nasal respiratory epithelium using scanning electron microscopy. In addition to the previously described ciliated, nonciliated, and goblet cells, the nasal brush cell was identified based on its surface characteristics and its location between nonciliated epithelial cells. Scanning electron microscopy clearly showed the differences in distribution of cell types in the nasal mucosa. The ciliated cells increase in number from the anterior to the posterior areas of the respiratory epithelium with a corresponding decrease in nonciliated cells. However, even at a single cross-sectional area of the nasal cavity, the various surfaces have different proportions of ciliated versus nonciliated cells, e.g., the medial surface of the nasal concha has more ciliated cells than other surfaces. Brush cells are distributed between nonciliated cells of the respiratory epithelium on most surfaces of the nasal cavity including the conchae and the lateral wall. Based on the available information, scanning electron microscopy will be useful in future studies to determine the effects that inhaled toxicants have on cells and on the location of lesions.

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“…Upon arrival, animals were placed in quarantine for 2 weeks; during this time it was determined that the rats were free of pathogenic viruses (standard rat virus titer screen; Microbiological Associates). Food and filter-purified tap water (29) were provided ad libitum, except during exposures; lighting was controlled by an automatic timer on a 12-hr light/12-hr dark cycle.…”

Section: Methodsmentioning

confidence: 99%

Role of epididymal inflammation in the induction of dominant lethal mutations in Fischer 344 rat sperm by methyl chloride.

Chellman¹,

Bus²,

Working³

1986

Proc. Natl. Acad. Sci. U.S.A.

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This study assessed the possible relationship between methyl chloride (MeCl)-induced epididymal inflammation and the formation of dominant lethal mutations in sperm of Fischer 344 rats. Groups of 40 males were exposed to MeCl (3000 ppm 6 hr/day for 5 days), with or without concurrent treatment with the anti-inflammatory agent 3-amino-1-[m-(trifluoromethyl)phenyl]-2-pyrazoline (BW 755C; 10 mg/kg, i.p. 1 hr pre-and postexposure); BW 755C was shown previously to inhibit MeCl-induced epididymal inflammation. Control groups (n = 20) were either untreated, injected as described above with BW 755C, or injected on the afternoon of day 5 with triethylenemelamine (0.2 mg/kg), a known dominant lethal mutagen. Each male was caged with one female weekly for 3 weeks; 12-18 days after mating, females were killed to assess dominant lethal parameters. In females bred to MeCl-exposed males, significant increases were observed in postimplantation loss at postexposure week 1 (0.84 dead implants per female vs. 0.29 in untreated controls) and in dead implants/total implants at both week 1 (0.10 vs. 0.04 control) and week 2 (0.24 vs. 0.06 control). These increases were not observed in females bred to males treated with BW 755C during MeCl exposure. Coadministration of BW 755C to males along with MeCl also reduced the percentage of mated females with two or more postimplantation losses from 31% to 8% (week 1) and 30% to 12% (week 2). Therefore, the dominant lethal mutations induced by MeCl appear to be a consequence of its induction of inflammation in the epididymis. These data demonstrate the potential genotoxicity of inflammatory processes in vivo.

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An evaluation of a water purification system for use in animal facilities

Cited by 15 publications

References 2 publications

Ultrastructural characterization of the nasal respiratory epithelium in the rat

Ultrastructural characterization of the nasal respiratory epithelium in the rat

Surface topography and distribution of cell types in the rat nasal respiratory epithelium: Scanning electron microscopic observations

Role of epididymal inflammation in the induction of dominant lethal mutations in Fischer 344 rat sperm by methyl chloride.

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