This study aimed to investigate the effect of core stabilization exercises on the contractile properties and isokinetic muscle function of adult females with a sedentary lifestyle. We enrolled 105 adult females. Tensiomyography was performed on the erector spinae, and the isokinetic muscular functional test was performed on the trunk at an angular velocity of 60°/s and 90°/s. All participants performed the exercise for 60 min per day, 3 times a week, for 7 weeks. A Wilcoxon signed-rank test was performed at a significance level of 0.05. Tensiomyography (TMG) of the erector spinae revealed no significant post-exercise change in the contraction time; however, there was a significant post-exercise increase in the maximum radial displacement and mean velocity until 90% of the TMG was displaced. Additionally, the isokinetic muscular functional test of the trunk revealed a significant post-exercise increase in almost all variables. Our findings demonstrated that the core stabilization exercise reduced stiffness in the erector spinae, increased the velocity of erector spinae contraction, and effectively improved the isokinetic muscular function of the trunk.