An evaluation of commercially available dehydrated Rappaport-Vassiliadis medium for the isolation of salmonellae from poultry

Abstract

SUMMARYA total of 745 samples of chicken giblets was cultured to determine the relative efficiency of a commercially available Rappaport-Vassiliadis medium (RV-Oxoid). Experiments to determine the optimum inoculation ratio showed that 1: 100 was superior to the other ratios tested. Comparison of RV-Oxoid with standard RV and RV-medium prepared using soya peptone (RV-soya) showed that after 24 h RV-soya was significantly better than RV-Oxoid (P < 0-05), although there was no significant difference between standard RV and RV-Oxoid. Furthermore, when the duration of incubation was extended to 48 h there was no significant difference between the three media (P > 0 25).We conclude that RV-Oxoid is a satisfactory product for the isolation of salmonellae from poultry, providing that it is inoculated at a ratio of 1: 100 and is incubated for 48h. Its use can therefore be recommended to laboratories who wish to use a dehydrated medium.

Selective action of inhibitors used in different culture media on the competitive microflora of Salmonella

“…Brilliant green is the stain most commonly used in culture media. Miles (1975) andFricker et al (1987) discussed the use of brilliant green to inhibit the growth of Proteus spp., a highly competitive bacteria with invasive growth on plates containing selective solid media. However, Fig.…”

The vertical transmission of salmonellas and formic acid treatment of chicken feed: A possible strategy for control

“…Microbiological examination of samples for salmonella Samples, 25 g, of all new batches of feed for adult hens, sampled at the mill, and many batches of broiler feed ingredients, including imported soya and fishmeal, were rehydrated in 225 ml buffered peptone water (BPW, Oxoid CM 509) and incubated at 37 C0 for 18 h. Samples were then treated as recommended by Fricker et al (1985). Litter samples, 100 g, were examined as above but using 11 of BPW.…”