An evaluation of fluorogenic and chromogenic assays for the direct enumeration of Escherichia coli

Ogden, I.D.; Watt, A.J.

doi:10.1111/j.1472-765x.1991.tb00610.x

Cited by 18 publications

(4 citation statements)

References 9 publications

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“…The samples were taken directly into a labeled sterile plastic container, kept in ice box and transported immediately for microbiological analysis at Sudanese Standard and Metrology organization-Red sea branch (SSMO) laboratory. Isolation and identification of E. coli was done according to the International Organization for Standardization [17]. Twenty five grams of fish muscle were homogenized with 225 ml buffer peptone water using a sterile pipette, transferred to a sterile container.…”

Section: A Samples' Collection and Processingmentioning

confidence: 99%

Risk Associate with E. coli in Marine Fish in Port Sudan, Red Sea State, Sudan

et al. 2022

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This study was carried to assess the risk associate with E. coli in marine fish in Port Sudan, Red Sea State, Sudan, through the production chain covering five stages. The stages were fish market, fish scaling, restaurant before cooking, and restaurant after cooking, and cooking and cooling for 24 h. A total of 50 fish samples were randomly selected from marine fish chain, in Port Sudan, Red Sea State, during the period from September to December 2018 to estimate the E. coli count using the TBX medium. In this study, the mean count of E. coli in the five stages of the fish chain was 257 +0.6 cfu. In fish market the mean was 247 +0.6 cfu, 758 +0.6 cfu in fish scaling stage, 283+0.6 cfu in restaurants before cooking, and 0.0 cfu after cooking and cooling for 24 hours after cooking. The chi-square test showed significant association between the load of E. coli among the fish chain and the most important potential risk factors (p-value ≤ 0.05), such as boxes cleaning (p-value= 0.000), hygienic practices (p-value= 0.002), waste disposal (p-value= 0.001), personal hygiene (p-value= 0.001), storage (p-value= 0.000) and general cleaning (p-value 0.002). The results of the frequencies of risk factors and their distribution in fish chain showed that 38% do not clean the boxes, 64% do not practice proper hygiene measures, 72% perform improper waste disposal, 72% had poor personal hygiene, 42% do not apply proper storage system, and 46% perform improper cleaning. All that will lead to medium overall risk estimation. Qualitative risk assessment for the E. coli in fish chain pathway was found to be high in the fish market and the exposure assessment in restaurant was found to be very low, so that the overall risk estimation for contamination with E. coli among the fish chain in Port Sudan was found to be Medium (The risky event is likely to occur more than once in the next three years).

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Section: A Samples' Collection and Processingmentioning

confidence: 99%

Risk Associate with E. coli in Marine Fish in Port Sudan, Red Sea State, Sudan

et al. 2022

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“…Isolation and identification of E. coli was done according to the International Organization for Standardization [5]. Twenty five grams of fish muscle was homogenized with 225 ml buffer peptone water using a sterile pipette, transferred to a sterile Petri dish.…”

Section: E Coli Isolation and Enumerationmentioning

confidence: 99%

“…According to [5], 25 g of fish muscle were homogenized with 225 ml buffer peptone water using sterile pipette. One ml of the test sample from initial suspension (10 -1 to 10 -5 dilutions), each dilution was transferred to two Petri dishes, by mean of another sterile Pipette 1 Ml of each decimal dilutions were poured in about 15 ml of the Violet Red Bile Agar (VRB) at 44°C to 47°C into each petri dish, allowed to solidify then the plates were incubated at 37°C for 24h+2.…”

Section: Coliform Enumerationmentioning

confidence: 99%

Enumeration of E. coli, Coliform and Aerobic Bacteria in Marine Fish in Port Sudan, Red Sea State, Sudan

Ali,

Salman,

Abubaker

et al. 2020

AJRAVS

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This study was carried out to detect the total bacterial count, coliform count and E. coli count through the production chain of marine fish (fish market, scaling, restaurant before cooking, restaurant after cooking and cooking and cooling for 24 h) in Port Sudan, Red Sea State, during the period from September to December 2018. A total of 50 fish samples were randomly selected from marine fish chain. Samples were subjected for bacteriological examination. The study showed that the mean count of E. coli in the five stages of the fish chain was 2.57×10 2 +0 .6 cfu, the mean coliform count was 2.01 ×10 3 + 0 .7 cfu/ g and the mean Aerobic Plate Count (APC) was found to be 3.6×10 6 + 0.09 cfu/ g. E. coli showed higher mean count (7.58×10 2) cfu/ g in the scaling stage. The

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“…O meio CHROMagar ECC possui 97% de sensibilidade para a detecção de bactérias do gênero E.Coli. (Ogden et al 1991 A coleta de amostras das superfícies foi realizada através da técnica de esfregaço utilizando "suabes" estéreis embebidos em água peptonada 0,5% estéril, foi amostrada uma superfície de 50 cm². Após esse procedimento, os "suabes" foram acondicionados em tubos de ensaio contendo 10mL de água peptonada 0,5% e imediatamente transportados ao laboratório para posterior diluição e incubação em placas de Petri.…”

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Avaliação do desempenho de frangos de corte e microbiota das instalações em dois programas de limpeza e desinfecção

Burbarelli¹

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Agradeço a Deus por todos os momentos vividos, desafios superados e pela saúde necessária para estes. Aos meus Pais Arlei e Neusa, pois sem a sua ajuda jamais teria realizado o sonho de ser Médica Veterinária. Aos meus avós, Olímpio e Pedrina que foram minha inspiração. A Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo. Ao departamento de Nutrição e Produção animal VNP. Ao Professor Ricardo pela orientação, exemplo e paciência. A Profa. Andrezza e Prof. Ricardo responsáveis pelo laboratório de Higiene Zootécnica da FZEA que gentilmente cederam suas instalações para realização das análises laboratoriais dos experimentos, além do apoio, conselhos e orientação. A Fapesp pela Bolsa e Auxilio Pesquisa oferecidos para execução do projeto. A imensa lista de pessoas especiais que ajudaram na execução do projeto: Carlos Eduardo-Cadu que ajudou diretamente na execução do projeto e me aguentou em todos os momentos de estresse e em que tudo parecia que iria por água abaixo. Jessica Russo que realizou parte do estágio obrigatório no Setor de Avicultura esteve presente na parte inicial deste projeto, diariamente.

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An evaluation of fluorogenic and chromogenic assays for the direct enumeration of Escherichia coli

Cited by 18 publications

References 9 publications

Risk Associate with E. coli in Marine Fish in Port Sudan, Red Sea State, Sudan

Risk Associate with E. coli in Marine Fish in Port Sudan, Red Sea State, Sudan

Enumeration of E. coli, Coliform and Aerobic Bacteria in Marine Fish in Port Sudan, Red Sea State, Sudan

Avaliação do desempenho de frangos de corte e microbiota das instalações em dois programas de limpeza e desinfecção

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