An evaluation of genotoxicity in human neuronal-type cells subjected to oxidative stress under an extremely low frequency pulsed magnetic field

Giorgi, Gianfranco; Lecciso, Mariangela; Capri, Miriam; Yani, Stella Lukas; Virelli, Angela; Bersani, Ferdinando; Re, Brunella Del

doi:10.1016/j.mrgentox.2014.10.003

Cited by 12 publications

(8 citation statements)

References 35 publications

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“…This is consistent with previous studies [31,32]. It is noted that in the literature, ELF-EMF fields frequently fail to induce DNA damage [33,34]. The two major DNA repair mechanisms for DSB, the more dominating type of DNA damage in our model, are homologous recombination (HR) and nonhomologous end-joining (NHEJ).…”

Section: Discussionsupporting

confidence: 92%

Induction of apoptosis and ferroptosis by a tumor suppressing magnetic field through ROS-mediated DNA damage

Yuan

Wang

et al. 2020

Aging

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Magnetic field (MF) is being used in antitumor treatment; however, the underlying biological mechanisms remain unclear. In this study, the potency and mechanism of a previously published tumor suppressing MF exposure protocol were further investigated. This protocol, characterized as a 50 Hz electromagnetic field modulated by static MF with time-average intensity of 5.1 mT, when applied for 2 h daily for over 3 consecutive days, selectively inhibited the growth of a broad spectrum of tumor cell lines including lung cancer, gastric cancer, pancreatic cancer and nephroblastoma. The level of intracellular reactive oxygen species (ROS) increased shortly after field exposure and persisted. Subsequently, pronounced DNA damage and activation of DNA repair pathways were identified both in vitro and in vivo. Furthermore, use of free radical scavenger alleviated DNA damage and partially reduced cell death. Finally, this field was found to inhibit cell proliferation, and simultaneously induced two types of programmed cell death, apoptosis and ferroptosis. In conclusion, this tumor suppressing MF could determine cell fate through ROS-induced DNA damage, inducing oxidative stress and activation of the DNA damage repair pathways, eventually lead to apoptosis and ferroptosis, as well as inhibition of tumor growth.

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Section: Discussionsupporting

confidence: 92%

Induction of apoptosis and ferroptosis by a tumor suppressing magnetic field through ROS-mediated DNA damage

Yuan

Wang

et al. 2020

Aging

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“…Among different electromagnetic field intensities that have been investigated, it has been shown that PEMFs are usually more effective when applied with an intensity of less than 3 mT [Bassett et al, ; Ryaby, ; Pesce et al, ]. Given that the field intensity of 1 mT was implemented in multiple studies for investigating the effects of PEMF on various cell types [Simkó et al, ; Lange et al, ; Lupke et al, ; Simkó and Mattsson, ; Giorgi et al, ; D'Angelo et al, ; Ardeshirylajimi and Khojasteh, ;], this intensity was chosen in the current in vitro study. The exposure system, similar to prior studies on PEMF [Calabrò et al, ; Dasdag et al, ; Haghnegahdar et al, ], was designed and provided by the Department of Nuclear Medicine, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.…”

Section: Methodsmentioning

confidence: 99%

The effects of electromagnetic fields on cultured human retinal pigment epithelial cells

Nasrabadi

Soheili

Bagheri

et al. 2018

Bioelectromagnetics

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A great deal of evidence has confirmed that electromagnetic fields (EMFs) can affect the central nervous system. In this study, cultured neonatal human retinal pigment epithelial (hRPE) cells were exposed to pulsed EMF of 1 mT intensity and 50 Hz frequency 8 h daily for 3 days. In addition to cell proliferation and cell death assays, immunocytochemistry for RPE65, PAX6, nestin, and cytokeratin 8/18 proteins were performed. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) was performed for NES, PAX6, RPE65, and ACTA2 gene expression. Exposed hRPE cells did not demonstrate significant change in terms of cytomorphology, cell proliferation, or cell death. Protein expression of PAX6 was decreased in treated cells compared to controls and remained unchanged for RPE65, cytokeratin 8/18, and nestin. Gene expressions of NES, RPE65, and PAX6 were decreased in treated cells as compared to controls. Gene expression of ACTA2 did not significantly change. In conclusion, viability of cultivated neonatal hRPE cells did not change after short exposure to a safe dose of pulsed EMF albeit that both gene and protein expressions of retinal progenitor cell markers were reduced. Whether longer exposure durations that are being constantly produced by widely-used electronic devices may induce significant changes in these cells, needs further investigation. Bioelectromagnetics. 39:585-594, 2018.

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“…Conforme Griffiths e outros (2006), o material genético é constantemente submetido a um cabo de guerra entre processos químicos que o danificam, levando a novas mutações, e os mecanismos de reparo que monitoram o DNA de forma constante a fim de corrigir os danos gerados. Considerando o fato de que lesões no material genético podem ser causadas por agentes químicos de diversas classes (POHREN;COSTA;VARGAS, 2013), a vida moderna permite a coexposição a diversos mutágenos que, juntos, podem agravar as lesões infringidas ao DNA, impedindo que a maquinaria celular aja eficazmente (GIORGI et al, 2014).…”

Section: Mutações: Consequência De Falhas Nos Mecanismos De Reparounclassified

Dna Barcoding Confirma a Ocorrência De Espécies Amazônicas Na Ictiofauna Do Rio Turiaçu, Maranhão/Brasil

Teixeira¹,

Barros²,

Fraga³

2019

Conceitos Básicos Da Genética

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An evaluation of genotoxicity in human neuronal-type cells subjected to oxidative stress under an extremely low frequency pulsed magnetic field

Cited by 12 publications

References 35 publications

Induction of apoptosis and ferroptosis by a tumor suppressing magnetic field through ROS-mediated DNA damage

Induction of apoptosis and ferroptosis by a tumor suppressing magnetic field through ROS-mediated DNA damage

The effects of electromagnetic fields on cultured human retinal pigment epithelial cells

Dna Barcoding Confirma a Ocorrência De Espécies Amazônicas Na Ictiofauna Do Rio Turiaçu, Maranhão/Brasil

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