“…Moreover they can be modified by chemical and physical processes in the atmosphere (Milne and Zika, 1993;McGregor and Anastasio, 2001;Franze et al, 2005;Haan et al, 2009). Therefore some authors only studied the total protein contribution through bicinchoninic acid assay (Micro BCA Protein Assay Kit) for the colorimetric detection and quantitation of the total proteins (Kang et al, 2012;Chen and Hildemann, 2009); or using the Nano-Orange Protein Quantification Kit (Molecular Probes, Eugene, OR) (Menetrez et al, 2007b;Menetrez et al, 2009;Mandalakis et al, 2011) or through water-soluble organic nitrogen analysis (Zhang et al, 2002). A method of determining combined amino acids in bioaerosols is the hydrolysis of peptides and proteins, followed by analysis of total amino acids (Samy et al, 2011).…”